Abstract：
AIM:To investigate the effect of hypoxia on the proliferation of mouse corneal epithelial cells in vitro. METHODS:Mouse corneal epithelial cells(MCEs) were cultured in normoxia (210mL/L O(2) and 50mL/L CO(2)) and hypoxia (20mL/L O(2) and 50mL/L CO(2)), respectively. Colony forming efficiency (CFE) and cell proliferation were determined. The expression of corneal epithelial progenitor cell marker p63 and K19 was investigated by immunostaining. RESULTS:Normoxic colonies were smaller compared with colonies formed in hypoxia. CFE was (12.50±1.50)% in hypoxic cultures, which was similar compared with normoxia cultures [(11.13±1.86)%, P>0.05)]. Cell proliferation was enhanced in hypoxia. Progenitor markers p63 and K19 were expressed in most cells under both normoxic and hypoxic conditions. CONCLUSION:Murine limbal epithelial progenitor cells can be efficiently expanded in hypoxic conditions.
journal_name
Int J Ophthalmoljournal_title
International journal of ophthalmologyauthors
Ma XL,Liu HQdoi
10.3980/j.issn.2222-3959.2011.02.07subject
Has Abstractpub_date
2011-01-01 00:00:00pages
147-9issue
2eissn
2222-3959issn
2227-4898pii
ijo-04-02-147journal_volume
4pub_type
杂志文章abstract：AIM:To compare the anterior segment measurements obtained by rotating Scheimpflug camera (Pentacam) and Scanning-slit topography (Orbscan IIz) in keratoconic eyes. METHODS:A total of 121 patients, 71 males (58.7%) and 50 females (41.3%) (214 eyes) with the diagnosis of keratoconus (KC) were enrolled in this study. Fol...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.02.11
更新日期：2015-04-18 00:00:00
abstract：AIM:To identify the genetic defects in a Chinese family with achromatopsia. METHODS:A 2.5-year-old boy, who displayed nystagmus, photophobia, and hyperopia since early infancy, was clinically evaluated. To further confirm and localize the causative mutations in this family, targeted region capture and next-generation ...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.05.10
更新日期：2015-10-18 00:00:00
abstract：AIM:To describe and compare the corneal endothelial cell density and morphology in young, low and moderate myopic Chinese adults in Malaysian Chinese population. METHODS:Non-contact specular microscopy (Topcon SP3000P, Tokyo, Japan) was performed in low (n=78; 21.22±1.51 years) and moderate (n=78; 21.82±1.40 years) my...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2013.04.11
更新日期：2013-08-18 00:00:00
abstract：AIM:To evaluate the rotational stability and visual outcomes of a single-piece hydrophilic acrylic toric intraocular lenses (IOL) over a 1.5-year follow-up period. Data from the 6-month follow-up study have been previously reported. METHODS:Forty eyes of 26 cataract patients (mean age: 72.8±7.9y) with pre-existing cor...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2017.04.12
更新日期：2017-04-18 00:00:00
abstract：AIM:To assess if the included vascular endothelial growth factor (VEGF) polymorphisms rs3025035, rs3025021 and rs2010963 are associated to proliferative retinopathy in a Mexican population with type 2 diabetes mellitus (T2DM). METHODS:A case-control study was conducted in adult individuals with T2DM associated to prol...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2017.01.22
更新日期：2017-01-18 00:00:00
abstract：AIM:To observe the curative effect of bandage contact lens in neurogenic keratitis. METHODS:Twenty cases of neurogenic keratitis were studied at the Department of Ophthalmology, the first Affiliated Hospital of China Medical University, between October 2012 and June 2013. These included 13 males and 7 females, aged fr...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2014.06.12
更新日期：2014-12-18 00:00:00
abstract：AIM:To observe the changes of basic fibroblast growth factor (bFGF) content in anterior chamber before and after extra capsular lens extraction for investigating the mechanism of low molecular weight heparin (LMWH) inhibiting anterior chamber inflammation. METHODS:Eighty-four rabbits were randomly divided into control...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2012.04.05
更新日期：2012-01-01 00:00:00
abstract：AIM:To investigate the effect of protein kinase C (PKC) on transforming growth factor-β(2) (TGFβ(2)) and dopamine in retinal Müller cells of guinea pig myopic eye. METHODS:Myopia was induced by translucent goggles in guinea pig, whose retinal Müller cells were cultured using the enzyme-digesting method. Retinal Müller...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2011.04.06
更新日期：2011-01-01 00:00:00
abstract：AIM:To assess the cost-effectiveness of autologous retinal pigment epithelium and choroid translocation (PATCH) in neovascular age-related macular degeneration (AMD). METHODS:Visual acuity and complication rates of published patient series were used to determine the incremental utility of treatment for the patient. Th...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2010.03.11
更新日期：2010-01-01 00:00:00
abstract：AIM:To study plasma levels of vascular endothelial growth factor (VEGF), endothelin-1(ET-1) and nitric oxide (NO) in patients with proliferative diabetic retinopathy (PDR) before and after pan-retinal photocoagulation (PRP). METHODS:Forty patients (23 females and 17 males, mean age 48.5±12.2 years) with PDR without pr...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2010.01.05
更新日期：2010-01-01 00:00:00
abstract：AIM:To compare posterior capsule opacification (PCO) degree and visual functions after phacoemulsification in eyes implanted with 360-degree square edge hydrophilic acrylic intraocular lens (IOL) (570C C-flex, Rayner) and sharp edge hydrophobic acrylic IOL (Sensar AR40e, AMO) in diabetic patients. METHODS:Sixty diabet...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.04.15
更新日期：2015-08-18 00:00:00
abstract：AIM:To investigate the cytotoxic effect on human corneal epithelial cells (HCECs) and the ability to faciliate corneal epithelial wound healing of carboxymethylcellulose (CMC) and hyaluronic acid (HA). METHODS:HCECs were exposed to 0.5% CMC (Refresh plus(®), Allergan, Irvine, California, USA) and 0.1% and 0.3% HA (Kyn...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.02.01
更新日期：2015-04-18 00:00:00
abstract：AIM:To prove anthrax lethal toxin (LeTx) blocks the mitogen activated protein kinases (MAPKs) activation by degrading the MAPK/ERK kinases (MEKs) to suppress vascular endothelial growth factor (VEGF) secretion. METHODS:Human adult retinal pigmented epithelium (ARPE) cells were cultured and treated with normal glucose,...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.03.04
更新日期：2015-06-18 00:00:00
abstract：AIM:To explore whether resveratrol (Res) can inhibit human retinal pigment epithelial cell (ARPE-19 cell) proliferation and migration, and to research the molecular mechanisms. METHODS:ARPE-19 cells were pretreated with various concentrations at 0, 50, 100, 150, 200 and 300 µmol/L of Res, and with 0 µmol/L Res as the ...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2016.12.04
更新日期：2016-12-18 00:00:00
abstract：AIM:To assess the anatomical and visual outcome of idiopathic macular holes greater than 1000 µm using the inverted internal limiting membrane flap technique. METHODS:This retrospective case series included 5 eyes of 5 patients with idiopathic macular hole with base diameter greater than 1000 µm who underwent inverted...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2016.08.22
更新日期：2016-08-18 00:00:00
abstract：AIM:To study the effect of different flap sizes on visual acuity, refractive outcomes, and aberrations after femtosecond laser for laser in situ keratomileusis (LASIK). METHODS:In each of the forty patients enrolled, 1 eye was randomly assigned to receive treatment with a 8.1mm diameter corneal flap, defined as the sm...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2013.05.16
更新日期：2013-10-18 00:00:00
abstract：AIM:To analyze the demography, presenting clinical features, spread of the disease of retinoblastoma in patients who were treated in two tertiary hospitals in Malaysia. METHODS:In this retrospective study, information of gender, age, race, presenting clinical features, findings of CT scan orbits and brain, lumbar punc...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2010.01.15
更新日期：2010-01-01 00:00:00
abstract：:Myopia, a worldwide condition, is a multifactorial disease resulting in many ocular complications. Early onset of myopia has a great tendency to develop high myopia and pathological myopia later in life. The pathophysiology and progression of myopia is still unclear. Owing to its involving in visual function, optic di...
journal_title：International journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.18240/ijo.2018.05.25
更新日期：2018-05-18 00:00:00
abstract：AIM:To investigate the effects of transforming growth factor β2 (TGF-β2) and connective tissue growth factor (CTGF) on transdifferentiation of human lens epithelial cells (HLECs) cultured in vitro and synthesis of extracellular matrix (ECM). METHODS:HLECs were treated with TGF-β2 (0, 0.5, 1.0, 5, 10µg/L) and CTGF (0, ...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2013.06.03
更新日期：2013-12-18 00:00:00
abstract：AIM:To assess the changes in the anterior chamber parameters using Pentacam following four different lines of treatment of primary angle-closure glaucoma (PACG). METHODS:A retrospective comparative study included 126 patients (126 eye) presented within 24-48h after acute angle-closure glaucoma (AACG). Patients were di...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2020.04.10
更新日期：2020-04-18 00:00:00
abstract：AIM:To explore the role of IgG4 serology in identifying common orbital lymphoproliferative disorders. METHODS:Eighty-one patients with orbital lymphoproliferative diseases were treated in the Department of Ocular Oncology, Beijing Tongren Hospital, Capital Medical University between September 2010 and December 2012. S...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2016.02.18
更新日期：2016-02-18 00:00:00
abstract：AIM:To investigate the published papers of ophthalmology in past ten years and explore the development of ophthalmology. METHORDS:The data of this study retrieved from Science Citation Index Expanded and downloaded online in November 2017, including all the papers with publication year from 2007-2016 were analyzed. Th...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2017.12.17
更新日期：2017-12-18 00:00:00
abstract：AIM:To identify metastatic genes and miRNAs and to investigate the metastatic mechanism of uveal melanoma (UVM). METHODS:GSE27831, GSE39717, and GSE73652 gene expression profiles were downloaded from the Gene Expression Omnibus (GEO) database, and the limma R package was used to identify differentially expressed genes...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2020.07.05
更新日期：2020-07-18 00:00:00
abstract：AIM:To study the effects of curcumin on the secretion of interleukin (IL)-6 and IL-8 by corneal limbus epithelial cells. METHODS:Human corneal limbus epithelial cells were isolated and cultured from donor eyes and irradiated by UVB at different dosages with or without curcumin. MTT test was used for studying the effec...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2017.06.01
更新日期：2017-06-18 00:00:00
abstract：AIM:To anatomically locate the points of minimum corneal thickness and central corneal thickness (pupil center) in relation to the corneal apex. METHODS:Observational, cross-sectional study, 299 healthy volunteers. Thickness at the corneal apex (AT), minimum corneal thickness (MT) and corneal thickness at the pupil ce...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2014.04.15
更新日期：2014-08-18 00:00:00
abstract：AIM:To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS:Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2016.09.06
更新日期：2016-09-18 00:00:00
abstract：AIM:To investigate the ocular hemodynamic effects of applying a hot compress to the eye. METHODS:The right eyes of five New Zealand white rabbits, both male and female, were hot-compressed for 18min. An independently designed novel ocular contact-type temperature measuring device was used to measure the ocular surface...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2018.12.04
更新日期：2018-12-18 00:00:00
abstract：AIM:To evaluate the effect of age on visual and refractive results after laser in situ keratomileusis (LASIK) obtained with a mechanical microkeratome or a femtosecond laser. METHODS:Retrospective, nonrandomized, cohort study. A total of 3826 eyes were included in the study (1725 eyes treated with mechanical LASIK and...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2019.03.21
更新日期：2019-03-18 00:00:00
abstract：AIM:To evaluate the measurements of anterior segment parameters using three different non-contact optical devices in keratoconus patients. METHODS:A hundred and one eyes of 55 keratoconus patients were enrolled in this study. The mean age was 26.2±8.9 years. The inclusion criteria were keratoconus stage I to III accor...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2013.04.21
更新日期：2013-08-18 00:00:00
abstract：AIM:To study the effect of Rac1 on the induction of HIF-1α in choroidal neovascularization (CNV) in mice. METHODS:One hundred C57BL/6J mice were laser photocoagulated to induce CNV, fifty mice of that were selected randomly for intravitreal injection of Rac1 inhibitor NSC23766 solution (1µL). After laser photocoagulat...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2011.01.03
更新日期：2011-01-01 00:00:00
