Abstract:
:Zinc deficiency impairs the immune system leading to frequent infections. Although zinc is known to play critical roles in maintaining healthy immune function, the underlying molecular targets are largely unknown. In this study, we demonstrate that zinc is important for the CD154-CD40-mediated activation of downstream signaling pathways in human B lymphocytes. CD40 is a receptor localized on the cell surface of many immune cells, including B lymphocytes. It binds to CD154, a membrane protein expressed on antigen-activated T helper (Th) lymphocytes. This CD154-CD40 interaction leads to B-cell activation. We showed that cellular zinc deficiency impaired the CD154-CD40-mediated p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation. We also showed that zinc supplemental treatment of B lymphocytes had limited effect on this CD40-mediated p38 MAPK signaling. Most importantly, we demonstrated that the zinc transporter protein zinc transporter 7 (ZNT7) interacted with CD40 using immunoprecipitation analyses. ZNT7 knockdown in B lymphocytes had a negative effect on the cell surface expression of CD40. Consequently, the CD40-mediated p38 MAPK signaling transduction was down-regulated in ZNT7 KD B lymphocytes. Conversely, this p38 MAPK signaling activity was up-regulated by overexpression (OE) of ZNT7 in B lymphocytes. Moreover, we found that ZNT7 knockdown in B lymphocytes constitutively up- and down-regulated the inhibitor of i kappa B kinase and AKT serine/threonine kinase phosphorylation, respectively, which implies the activation of survival signaling in ZNT7 KD B cells. We conclude that CD40 is the target molecule for ZNT7 in regulation of immune function of B lymphocytes.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Tepaamorndech S,Oort P,Kirschke CP,Cai Y,Huang Ldoi
10.1002/2211-5463.12211subject
Has Abstractpub_date
2017-03-27 00:00:00pages
675-690issue
5issn
2211-5463pii
FEB412211journal_volume
7pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that FAD treatme...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12331
更新日期:2017-10-17 00:00:00
abstract::The Wilms tumor protein 1 (WT1) transcription factor has been associated in malignant melanoma with cell survival and metastasis, thus emerging as a candidate for targeted therapy. A lysine-arginine rich peptide, WT1-pTj, derived from the ZF domain of WT1 was evaluated as an antitumor agent against A2058 human melanom...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.01.007
更新日期:2014-01-21 00:00:00
abstract::Thioredoxin interacting protein (TXNIP) is a novel tumor suppressor that is down-regulated in several cancer tissues and tumor cell lines. Overexpression of TXNIP causes cell cycle arrest at the G1/S checkpoint in the hepatocellular carcinoma cell line HuH-7. TXNIP contains putative phosphorylation sites, but the effe...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12518
更新日期:2018-10-01 00:00:00
abstract::miR-193a-3p is a tumor-related miRNA playing an essential role in tumorigenesis and progression of non-small cell lung cancer (NSCLC). The objective of the present study was to investigate the relationship between miR-193a-3p expression and clinical value and to further explore the potential signaling of miR-193a-3p i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12354
更新日期:2018-01-04 00:00:00
abstract::In this study, we showed that cyanocobalamin dodecylamine, a ribose 5'-carbamate derivative of cyanocobalamin, was absorbed and accumulated to significant levels by Caenorhabditis elegans and was not further metabolized. The levels of methylmalonic acid and homocysteine, which serve as indicators of cobalamin deficien...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.07.008
更新日期:2014-08-01 00:00:00
abstract::Baicalin (5,6-dihydroxy-7-o-glucuronide flavone) is an extract from the roots of Chinese herb Huang Qin (Scutellaria baicalensis Georgi) and is reported to have antioxidative, antiproliferative, anti-inflammatory, and anticancer activities. This study aimed to investigate the inhibitory effect of baicalin on human hep...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12130
更新日期:2016-10-24 00:00:00
abstract::Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.05.001
更新日期:2013-05-30 00:00:00
abstract::Glutathione transferases (GSTs) are involved in many processes in plant biochemistry, with their best characterised role being the detoxification of xenobiotics through their conjugation with glutathione. GSTs have also been implicated in noncatalytic roles, including the binding and transport of small heterocyclic li...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12168
更新日期:2016-12-22 00:00:00
abstract::Postoperative cognitive dysfunction is a common complication in elderly patients after surgeries involving anesthesia, but the underlying mechanisms are poorly understood. Lithium is a conventional treatment for bipolar disorder, which exerts a neuroprotective role in various diseases by inhibiting glycogen synthase k...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12779
更新日期:2019-12-22 00:00:00
abstract::Prostate cancer (PCa) is the most frequently diagnosed male cancer. An earlier study of a cohort of 333 primary prostate carcinomas showed that 74% of these tumors fell into one of seven subtypes of a molecular taxonomy defined by specific gene fusions (ERG, ETV1/4 and FLI1) or mutations (SPOP, FOXA1 and IDH1). Molecu...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13004
更新日期:2020-12-01 00:00:00
abstract::Photodynamic therapy is an advanced method of treating cancer and various benign diseases, including infections. It uses light-activated molecules [photosensitizers (PSs)] to generate reactive oxygen species (ROS) when irradiated with light of a specific wavelength. This study examined the photophysical and photosensi...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12359
更新日期:2018-01-02 00:00:00
abstract::Cisplatin-based chemotherapy is the standard treatment of choice for head and neck squamous cell carcinoma (HNSCC). The efficiency of platinum-based therapies is directly influenced by the development of tumor resistance. Multiple signaling pathways have been linked to tumor resistance, including activation of nuclear...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.12.003
更新日期:2013-12-30 00:00:00
abstract::Epigallocatechin gallate (EGCG), a major polyphenol in green tea, possesses antioxidant properties and regulates various cell functions. Here, we examined the function of EGCG in inflammatory bone resorption. In calvarial organ cultures, lipopolysaccharide (LPS)-induced bone resorption was clearly suppressed by EGCG. ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.003
更新日期:2015-06-12 00:00:00
abstract::In this study, it was shown for the first time that l-amino acid oxidase of Pseudomonas sp. AIU813, renamed as l-amino acid oxidase/monooxygenase (l-AAO/MOG), exhibits l-lysine 2-monooxygenase as well as oxidase activity. l-Lysine oxidase activity of l-AAO/MOG was increased in a p-chloromercuribenzoate (p-CMB) concent...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.02.002
更新日期:2014-02-07 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most malignant form of brain tumor and is associated with resistance to conventional therapy and poor patient survival. Prostate apoptosis response (Par)-4, a tumor suppressor, is expressed as both an intracellular and secretory/extracellular protein. Though secretory Par-4 induces...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.11.005
更新日期:2014-11-21 00:00:00
abstract::Despite progress in the treatment of hepatocellular carcinoma (HCC), 5-year survival rates remain low. Thus, a more comprehensive approach to explore the mechanism of HCC is needed to provide new leads for targeted therapy. We performed an integrated analysis to discover the relationship between DNA methylation and ge...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12433
更新日期:2018-05-30 00:00:00
abstract::Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene e...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12386
更新日期:2018-02-19 00:00:00
abstract::Dedifferentiation of adipocytes contributes to the generation of a proliferative cell population that could be useful in cellular therapy or tissue engineering. Adipocytes can dedifferentiate into precursor cells to acquire a fibroblast-like phenotype using ceiling culture, in which the buoyancy of fat cells is exploi...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12250
更新日期:2017-07-10 00:00:00
abstract::Neural stem cells (NSC) from the adult hippocampus easily lose their activity in vitro. Efficient in vitro expansion of adult hippocampus-derived NSC is important for generation of tools for research and cell therapy. Here, we show that a single copy disruption or pharmacological inhibition of p38α enables successful ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.05.001
更新日期:2015-05-08 00:00:00
abstract::Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unc...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.08.009
更新日期:2015-08-29 00:00:00
abstract::Redox-coupled folding pathways of bovine pancreatic ribonuclease A (RNase A) with four intramolecular disulfide (SS) bonds comprise three phases: (I) SS formation to generate partially oxidized intermediate ensembles with no rigid folded structure; (II) SS rearrangement from the three SS intermediate ensemble (3S) to ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.04.001
更新日期:2012-04-16 00:00:00
abstract::Liver cancer stem cells (L-CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long-term culture model for a specific subpopulation of L-CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L-CSCs, w...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12932
更新日期:2020-09-01 00:00:00
abstract::IL-1 family cytokines act as apical initiators of inflammation in many settings and can promote the production of a battery of inflammatory cytokines, chemokines and other inflammatory mediators in diverse cell types. IL-36α, IL-36β and IL-36γ, which belong to the extended IL-1 family, have been implicated as key init...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12406
更新日期:2018-03-25 00:00:00
abstract::In the brain, REST (Repressor Element-1 Silencing Transcription factor) is a key regulator of neuron cell-specific gene expression. Nuclear translocation of neuronal REST has been shown to be neuroprotective in a healthy ageing context. In contrast, inability to upregulate nuclear REST is thought to leave ageing neuro...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13036
更新日期:2020-11-13 00:00:00
abstract::The A/B domains of nuclear receptors such as thyroid receptor α (TRα) are considered to be conformationally flexible and can potentially adopt multiple structural conformations. We used intrinsic tryptophan fluorescence quenching and circular dichroism spectroscopy to characterize the unfolding of this A/B domain upon...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12229
更新日期:2017-05-15 00:00:00
abstract::Y-box-binding protein 1 (YB1) is a multifunctional transcription factor with vital roles in proliferation, differentiation and apoptosis. In this study, we have examined the role of its C-terminal domain (YB1 CTD) in proliferation, angiogenesis and tumorigenicity in breast cancer. Breast cancer cell line SK-BR-3 was i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12004
更新日期:2016-01-11 00:00:00
abstract::The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12962
更新日期:2020-10-01 00:00:00
abstract::Mediterranean Carthamus tinctorius (Safflower) is used for treatment of inflammatory conditions and neuropsychiatric disorders. Recently C. tinctorius lignans arctigenin and trachelogenin but not matairesinol were described to interfere with the activity of tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO)...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.08.008
更新日期:2013-09-07 00:00:00
abstract::The yeast cyclins Cln1 and Cln2 are very similar in both sequence and function, but some differences in their functionality and localization have been recently described. The control of Cln1 and Cln2 cellular levels is crucial for proper cell cycle initiation. In this work, we analyzed the degradation patterns of Cln1...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12157
更新日期:2016-12-14 00:00:00
abstract::The identification of biomarkers for early diagnosis of Parkinson's disease (PD) prior to the onset of symptoms may improve the effectiveness of therapy. To identify potential biomarkers, we downloaded microarray datasets of PD from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PD...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12687
更新日期:2019-08-01 00:00:00