Abstract:
:The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy reconstruction of the native large subunit at subnanometer resolution. The insertion site forms a four-way junction that fully preserves the canonical 5S rRNA structure. Moving away from the junction site, the inserted region is conformationally flexible and does not pack tightly against the large subunit. The high-salt requirement of the H. morrhuae ribosomes for their stability conflicted with the low-salt threshold for cryo-electron microscopy procedures. Despite this obstacle, this is the first cryo-electron microscopy map of Halococcus ribosomes.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Tirumalai MR,Kaelber JT,Park DR,Tran Q,Fox GEdoi
10.1002/2211-5463.12962subject
Has Abstractpub_date
2020-10-01 00:00:00pages
1938-1946issue
10issn
2211-5463journal_volume
10pub_type
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