In vivo assay to monitor flavonoid uptake across plant cell membranes.


:Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2-aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non-pigmented Vitis vinifera cell cultures.


FEBS Open Bio


FEBS open bio


Filippi A,Petrussa E,Peresson C,Bertolini A,Vianello A,Braidot E




Has Abstract


2015-08-29 00:00:00










  • Mutational and crystallographic analysis of l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities.

    abstract::In this study, it was shown for the first time that l-amino acid oxidase of Pseudomonas sp. AIU813, renamed as l-amino acid oxidase/monooxygenase (l-AAO/MOG), exhibits l-lysine 2-monooxygenase as well as oxidase activity. l-Lysine oxidase activity of l-AAO/MOG was increased in a p-chloromercuribenzoate (p-CMB) concent...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Matsui D,Im DH,Sugawara A,Fukuta Y,Fushinobu S,Isobe K,Asano Y

    更新日期:2014-02-07 00:00:00

  • The high-risk HPV E6 proteins modify the activity of the eIF4E protein via the MEK/ERK and AKT/PKB pathways.

    abstract::Previous studies have proposed that the human papillomavirus (HPV) E6 oncoproteins modify the transcriptional activity of eIF4E through mechanisms dependent on p53 degradation. However, the effect of these oncoproteins on pathways regulating the activity of the eIF4E protein remains poorly understood. Hence, we invest...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Morales-Garcia V,Contreras-Paredes A,Martinez-Abundis E,Gomez-Crisostomo NP,Lizano M,Hernandez-Landero F,de la Cruz-Hernandez E

    更新日期:2020-12-01 00:00:00

  • Rosiglitazone suppresses RANKL-induced NFATc1 autoamplification by disrupting the physical interaction between NFATc1 and PPARγ.

    abstract::Receptor activator of nuclear factor-κB ligand (RANKL) is required for initiation of osteoclastogenesis, with the signaling pathway including the NF-kB, c-Fos, and nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) transcription factors. Because NFATc1 expression is autoamplified, we investigated the molecula...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Park KL,Oh DG,Kim YO,Song KS,Ahn DW

    更新日期:2018-09-10 00:00:00

  • Crystal structure of the retroviral protease-like domain of a protozoal DNA damage-inducible 1 protein.

    abstract::DNA damage-inducible 1 (Ddi1) is a multidomain protein with one of the domains being retropepsin-like. HIV-1 protease inhibitors were found to reduce opportunistic infections caused by pathogens like Leishmania and Plasmodium, and some of them were shown to inhibit the growth of these parasites. In Leishmania, Ddi1 wa...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Kumar S,Suguna K

    更新日期:2018-08-03 00:00:00

  • A risk score model for the prediction of osteosarcoma metastasis.

    abstract::Osteosarcoma is the most common primary solid malignancy of the bone, and its high mortality usually correlates with early metastasis. In this study, we developed a risk score model to help predict metastasis at the time of diagnosis. We downloaded and mined four expression profile datasets associated with osteosarcom...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Dong S,Huo H,Mao Y,Li X,Dong L

    更新日期:2019-02-02 00:00:00

  • Niclosamide and its derivative DK-520 inhibit RANKL-induced osteoclastogenesis.

    abstract::Niclosamide is a potent inhibitor of osteoclastogenesis and bone remodeling. DK-520 is an acyl derivative of Niclosamide and significantly increased both the plasma concentration and the duration of exposure of Niclosamide when dosed orally. However, at present the effect of DK-520 on osteoclastogenesis has not been r...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Jiao Y,Chen C,Hu X,Feng X,Shi Z,Cao J,Li Q,Zhu Y

    更新日期:2020-08-01 00:00:00

  • An in vivo system involving co-expression of cyanobacterial flavodoxin and ferredoxin-NADP(+) reductase confers increased tolerance to oxidative stress in plants.

    abstract::Oxidative stress in plants causes ferredoxin down-regulation and NADP(+) shortage, over-reduction of the photosynthetic electron transport chain, electron leakage to oxygen and generation of reactive oxygen species (ROS). Expression of cyanobacterial flavodoxin in tobacco chloroplasts compensates for ferredoxin declin...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Giró M,Ceccoli RD,Poli HO,Carrillo N,Lodeyro AF

    更新日期:2011-11-11 00:00:00

  • A novel assay for measuring recombinant human lysophosphatidylcholine acyltransferase 3 activity.

    abstract::Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is an important enzyme in phospholipid remodeling, a process that influences the biophysical properties of cell membranes and thus cell function. Multiple lines of evidence suggest that LPCAT3 is involved in several diseases, including atherosclerosis, non-alcoholic s...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Du X,Hu J,Zhang Q,Liu Q,Xiang X,Dong J,Lou B,He S,Gu X,Cao Y,Li Y,Ding T

    更新日期:2019-10-01 00:00:00

  • Fluoride resistance capacity in mammalian cells involves complex global gene expression changes.

    abstract::Fluorine is a bone-seeking element ubiquitously present in the environment and widely used in many oral hygiene products. In humans, excessive intake of fluoride may cause dental and skeletal fluorosis. However, endemic fluorosis does not appear to develop in a proportion of individuals exposed to the same levels of f...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Ran S,Sun N,Liu Y,Zhang W,Li Y,Wei L,Wang J,Liu B

    更新日期:2017-06-05 00:00:00

  • The gene expression of numerous SLC transporters is altered in the immortalized hypothalamic cell line N25/2 following amino acid starvation.

    abstract::Amino acids are known to play a key role in gene expression regulation, and in mammalian cells, amino acid signaling is mainly mediated via two pathways, the mammalian target of rapamycin complex 1 (mTORC1) pathway and the amino acid responsive (AAR) pathway. It is vital for cells to have a system to sense amino acid ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Hellsten SV,Lekholm E,Ahmad T,Fredriksson R

    更新日期:2017-01-11 00:00:00

  • Functional and structural characterization of HspB1/Hsp27 from Chinese hamster ovary cells.

    abstract::Small heat shock proteins (sHsps) endow cells with stress tolerance. Of the various sHsps in mammals, HspB1, also known as Hsp27, is the most ubiquitous. To examine the structure and function of HspB1, we expressed, purified, and characterized HspB1 from Chinese hamster (Cricetulus griseus) ovary cells (CgHspB1). CgHs...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Sha E,Nakamura M,Ankai K,Yamamoto YY,Oka T,Yohda M

    更新日期:2019-10-01 00:00:00

  • Cyclophilin D-dependent mitochondrial permeability transition amplifies inflammatory reprogramming in endotoxemia.

    abstract::Microorganisms or LPS (lipopolysaccharide), an outer membrane component of Gram-negative bacteria, can induce a systemic inflammatory response that leads to sepsis, multiple organ dysfunction, and mortality. Here, we investigated the role of cyclophilin D (CypD)-dependent mitochondrial permeability transition (mPT) in...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Veres B,Eros K,Antus C,Kalman N,Fonai F,Jakus PB,Boros E,Hegedus Z,Nagy I,Tretter L,Gallyas F Jr,Sumegi B

    更新日期:2021-01-20 00:00:00

  • The clinical value of miR-193a-3p in non-small cell lung cancer and its potential molecular mechanism explored in silico using RNA-sequencing and microarray data.

    abstract::miR-193a-3p is a tumor-related miRNA playing an essential role in tumorigenesis and progression of non-small cell lung cancer (NSCLC). The objective of the present study was to investigate the relationship between miR-193a-3p expression and clinical value and to further explore the potential signaling of miR-193a-3p i...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Gao X,Tang RX,Xie QN,Lin JY,Shi HL,Chen G,Li ZY

    更新日期:2018-01-04 00:00:00

  • Ornithine cyclodeaminase/μ-crystallin homolog from the hyperthermophilic archaeon Thermococcus litoralis functions as a novel Δ(1)-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-l-proline metabolism.

    abstract::l-Ornithine cyclodeaminase (OCD) is involved in l-proline biosynthesis and catalyzes the unique deaminating cyclization of l-ornithine to l-proline via a Δ(1)-pyrroline-2-carboxyrate (Pyr2C) intermediate. Although this pathway functions in only a few bacteria, many archaea possess OCD-like genes (proteins), among whic...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Watanabe S,Tozawa Y,Watanabe Y

    更新日期:2014-07-10 00:00:00

  • Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC.

    abstract::Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Khusainov R,Moll GN,Kuipers OP

    更新日期:2013-05-30 00:00:00

  • Septal protein SepJ from the heterocyst-forming cyanobacterium Anabaena forms multimers and interacts with peptidoglycan.

    abstract::Heterocyst-forming cyanobacteria grow as filaments that can be hundreds of cells long. Proteinaceous septal junctions provide cell-cell binding and communication functions in the filament. In Anabaena sp. strain PCC 7120, the SepJ protein is important for the formation of septal junctions. SepJ consists of integral me...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Ramos-León F,Mariscal V,Battchikova N,Aro EM,Flores E

    更新日期:2017-08-30 00:00:00

  • Epigenetic regulation of regulatory T cells in patients with abdominal aortic aneurysm.

    abstract::Abdominal arterial aneurysm (AAA) shares many features with autoimmune diseases and appears to be a T-cell-mediated process. In addition, certain epigenetic changes, including DNA methylation, are associated with AAA. In this study, we investigated epigenetic modifications in regulatory T cells (Tregs) from AAA patien...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Xia Q,Zhang J,Han Y,Zhang X,Jiang H,Lun Y,Wu X,Gang Q,Liu Z,Böckler D,Duan Z,Xin S

    更新日期:2019-06-01 00:00:00

  • Epigallocatechin gallate (EGCG) suppresses lipopolysaccharide-induced inflammatory bone resorption, and protects against alveolar bone loss in mice.

    abstract::Epigallocatechin gallate (EGCG), a major polyphenol in green tea, possesses antioxidant properties and regulates various cell functions. Here, we examined the function of EGCG in inflammatory bone resorption. In calvarial organ cultures, lipopolysaccharide (LPS)-induced bone resorption was clearly suppressed by EGCG. ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Tominari T,Matsumoto C,Watanabe K,Hirata M,Grundler FM,Miyaura C,Inada M

    更新日期:2015-06-12 00:00:00

  • Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing.

    abstract::One practical application of genome-scale metabolic reconstructions is to interrogate multispecies relationships. Here, we report a consensus metabolic model in four yeast species (Saccharomyces cerevisiae, S. paradoxus, S. mikatae, and S. bayanus) by integrating metabolic network simulations with RNA sequencing (RNA-...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Zhao Y,Wang Y,Zou L,Huang J

    更新日期:2016-02-27 00:00:00

  • Development of a novel murine heart failure model overexpressing human renin and angiotensinogen.

    abstract::Renin is the rate-limiting enzyme of the renin-angiotensin system cascade, which drives the pathophysiological progression of heart failure. Species differences in the amino acid sequence of the catalytic domain of renin limit evaluations of the potency and efficacy of human renin inhibitors in animal models, and a hi...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Hara T,Yamamura T,Murakami-Asahina M,Matsumoto H,Takeyama M,Kanagawa R,Nishimoto T

    更新日期:2020-05-01 00:00:00

  • Characterisation of a novel cold-adapted calcium-activated transglutaminase: implications for medicine and food processing.

    abstract::Transglutaminases are a family of enzymes that catalyse the cross-linking of proteins by forming covalent bonds between lysine and glutamine residues in various polypeptides. Cross-linking reactions are involved in blood clots, skin formation, embryogenesis and apoptosis. Clinically, these enzymes appear to be implica...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Alvarez RG,Karki P,Langleite IE,Bakksjø RJ,Eichacker LA,Furnes C

    更新日期:2020-04-01 00:00:00

  • Spatiotemporal immunolocalisation of REST in the brain of healthy ageing and Alzheimer's disease rats.

    abstract::In the brain, REST (Repressor Element-1 Silencing Transcription factor) is a key regulator of neuron cell-specific gene expression. Nuclear translocation of neuronal REST has been shown to be neuroprotective in a healthy ageing context. In contrast, inability to upregulate nuclear REST is thought to leave ageing neuro...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Mampay M,Velasco-Estevez M,Rolle SO,Chaney AM,Boutin H,Dev KK,Moeendarbary E,Sheridan GK

    更新日期:2020-11-13 00:00:00

  • BBOX1-AS1 contributes to colorectal cancer progression by sponging hsa-miR-361-3p and targeting SH2B1.

    abstract::Colorectal cancer (CRC) is the third main cause of cancer-relevant deaths worldwide, and its incidence has increased in recent decades. Previous studies have indicated that certain long non-coding RNAs (lncRNAs) have regulatory roles in tumor occurrence and progression. Often, lncRNAs are competitive endogenous RNAs (...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Liu J,Zhu J,Xiao Z,Wang X,Luo J

    更新日期:2020-01-27 00:00:00

  • Crystal structures of FMN-bound and FMN-free forms of dihydroorotate dehydrogenase from Trypanosoma brucei.

    abstract::Dihydroorotate dehydrogenase (DHODH) is a flavin-binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three-dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes poss...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Kubota T,Tani O,Yamaguchi T,Namatame I,Sakashita H,Furukawa K,Yamasaki K

    更新日期:2018-03-06 00:00:00

  • A novel cell-penetrating peptide derived from WT1 enhances p53 activity, induces cell senescence and displays antimelanoma activity in xeno- and syngeneic systems.

    abstract::The Wilms tumor protein 1 (WT1) transcription factor has been associated in malignant melanoma with cell survival and metastasis, thus emerging as a candidate for targeted therapy. A lysine-arginine rich peptide, WT1-pTj, derived from the ZF domain of WT1 was evaluated as an antitumor agent against A2058 human melanom...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Massaoka MH,Matsuo AL,Figueiredo CR,Girola N,Faria CF,Azevedo RA,Travassos LR

    更新日期:2014-01-21 00:00:00

  • Kinetic and thermodynamic analysis of the conformational folding process of SS-reduced bovine pancreatic ribonuclease A using a selenoxide reagent with high oxidizing ability.

    abstract::Redox-coupled folding pathways of bovine pancreatic ribonuclease A (RNase A) with four intramolecular disulfide (SS) bonds comprise three phases: (I) SS formation to generate partially oxidized intermediate ensembles with no rigid folded structure; (II) SS rearrangement from the three SS intermediate ensemble (3S) to ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Arai K,Kumakura F,Iwaoka M

    更新日期:2012-04-16 00:00:00

  • Role of the TGF-β pathway in dedifferentiation of human mature adipocytes.

    abstract::Dedifferentiation of adipocytes contributes to the generation of a proliferative cell population that could be useful in cellular therapy or tissue engineering. Adipocytes can dedifferentiate into precursor cells to acquire a fibroblast-like phenotype using ceiling culture, in which the buoyancy of fat cells is exploi...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Côté JA,Lessard J,Pelletier M,Marceau S,Lescelleur O,Fradette J,Tchernof A

    更新日期:2017-07-10 00:00:00

  • Serum amyloid A1 (SAA1) protein in human colostrum.

    abstract::Proteins of the serum amyloid A (SAA) family have been remarkably conserved in evolution. Their biologic function(s) are not fully defined but they are likely to be a part of primordial host defense. We have detected a ∼ 12-kDa protein reacting with antibodies against serum amyloid A (SAA) in human colostrum by wester...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Sack GH Jr,Zachara N,Rosenblum N,Talbot CC Jr,Kreimer S,Cole R,McDonald TL

    更新日期:2018-01-31 00:00:00

  • Bioinformatic analysis of the fold type I PLP-dependent enzymes reveals determinants of reaction specificity in l-threonine aldolase from Aeromonas jandaei.

    abstract::Understanding the role of specific amino acid residues in the molecular mechanism of a protein's function is one of the most challenging problems in modern biology. A systematic bioinformatic analysis of protein families and superfamilies can help in the study of structure-function relationships and in the design of i...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Fesko K,Suplatov D,Švedas V

    更新日期:2018-05-21 00:00:00

  • miR-21-5p promotes cell proliferation and G1/S transition in melanoma by targeting CDKN2C.

    abstract::Human melanoma is a highly malignant tumor originating from cutaneous melanocytes. The noncoding RNA microRNA (miR)-21-5p has been reported to be expressed at high levels in malignant melanocytic skin tissues, but its potential functional role in melanoma remains poorly understood. Here, we explored the cellular effec...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Yang Z,Liao B,Xiang X,Ke S

    更新日期:2020-05-01 00:00:00