In vivo assay to monitor flavonoid uptake across plant cell membranes.

Abstract:

:Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2-aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non-pigmented Vitis vinifera cell cultures.

journal_name

FEBS Open Bio

journal_title

FEBS open bio

authors

Filippi A,Petrussa E,Peresson C,Bertolini A,Vianello A,Braidot E

doi

10.1016/j.fob.2015.08.009

subject

Has Abstract

pub_date

2015-08-29 00:00:00

pages

748-52

issn

2211-5463

pii

S2211-5463(15)00082-0

journal_volume

5

pub_type

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