Abstract:
:Dedifferentiation of adipocytes contributes to the generation of a proliferative cell population that could be useful in cellular therapy or tissue engineering. Adipocytes can dedifferentiate into precursor cells to acquire a fibroblast-like phenotype using ceiling culture, in which the buoyancy of fat cells is exploited to allow them to adhere to the inner surface of a container. Ceiling culture is usually performed in flasks, which limits the ability to test various culture conditions. Using a new six-well plate ceiling culture approach, we examined the relevance of TGF-β signaling during dedifferentiation. Adipose tissue samples from patients undergoing bariatric surgery were digested with collagenase, and cell suspensions were used for ceiling cultures. Using the six-well plate approach, cells were treated with SB431542 (an inhibitor of TGF-β receptor ALK5) or human TGF-β1 during dedifferentiation. Gene expression was measured in these cultures and in whole adipose tissue, the stromal-vascular fraction (SVF), mature adipocytes, and dedifferentiated fat (DFAT) cells. TGF-β1 and collagen type I alpha 1 (COL1A1) gene expression was significantly higher in DFAT cells compared to whole adipose tissue samples and SVF cells. TGF-β1, COL1A1, and COL6A3 gene expression was significantly higher at day 12 of dedifferentiation compared to day 0. In the six-well plate model, treatment with TGF-β1 or SB431542, respectively, stimulated and inhibited the TGF-β pathway as shown by increased TGF-β1, TGF-β2, COL1A1, and COL6A3 gene expression and decreased expression of TGF-β1, COL1A1, COL1A2, and COL6A3, respectively. Treatment of DFAT cells with TGF-β1 increased the phosphorylation level of SMAD 2 and SMAD 3. Thus, a new six-well plate model for ceiling culture allowed us to demonstrate a role for TGF-β in modulating collagen gene expression during dedifferentiation of mature adipocytes.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Côté JA,Lessard J,Pelletier M,Marceau S,Lescelleur O,Fradette J,Tchernof Adoi
10.1002/2211-5463.12250subject
Has Abstractpub_date
2017-07-10 00:00:00pages
1092-1101issue
8issn
2211-5463pii
FEB412250journal_volume
7pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::Mediterranean Carthamus tinctorius (Safflower) is used for treatment of inflammatory conditions and neuropsychiatric disorders. Recently C. tinctorius lignans arctigenin and trachelogenin but not matairesinol were described to interfere with the activity of tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO)...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.08.008
更新日期:2013-09-07 00:00:00
abstract::Micro-exons are a set of ultrashort exons with lengths ≤ 51 nucleotides. Our previous study revealed that micro-exons were enriched in AP2 domains and K-box domains, which are crucial components of AP2/ERF (APETALA2/ethylene-responsive element-binding protein) and MADS-box (an acronym of MCM1, AGAMOUS, DEFICIENS and S...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12990
更新日期:2020-12-01 00:00:00
abstract::Memory includes the processes of acquisition, consolidation and retrieval. In the study of aversive olfactory memory in Drosophila melanogaster, flies are first exposed to an odor (conditioned stimulus, CS+) that is associated with an electric shock (unconditioned stimulus, US), then to another odor (CS-) without the ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12203
更新日期:2017-02-22 00:00:00
abstract::As a small fruit rich in anthocyanins, blueberry (Vaccinium corymbosum × angustifolium) has become a focus of research in recent years for identifying genes related to anthocyanin transport and stress resistance mechanisms based on transcriptome sequencing. However, the lack of validated, stably expressed reference ge...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12903
更新日期:2020-08-01 00:00:00
abstract::The expression of death receptor 6 (DR6) is abnormal in some cancer types, but the function and underlying molecular mechanisms of DR6 in tumor progression are not yet clear. In the present study, our analysis of ovarian cancer RNA sequencing data from The Cancer Genome Atlas revealed that DR6 is upregulated in human ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12492
更新日期:2018-08-07 00:00:00
abstract::Abdominal arterial aneurysm (AAA) shares many features with autoimmune diseases and appears to be a T-cell-mediated process. In addition, certain epigenetic changes, including DNA methylation, are associated with AAA. In this study, we investigated epigenetic modifications in regulatory T cells (Tregs) from AAA patien...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12643
更新日期:2019-06-01 00:00:00
abstract::Human melanoma is a highly malignant tumor originating from cutaneous melanocytes. The noncoding RNA microRNA (miR)-21-5p has been reported to be expressed at high levels in malignant melanocytic skin tissues, but its potential functional role in melanoma remains poorly understood. Here, we explored the cellular effec...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12819
更新日期:2020-05-01 00:00:00
abstract::Sorafenib is an inhibitor of a variety of tyrosine kinase receptors used to treat various cancers including hepatocellular, renal cell and thyroid carcinoma. It has been shown to change various targets associated with osteosarcoma, but the detailed mechanism remains unclear. In order to identify key genes, enriched pa...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12428
更新日期:2018-04-24 00:00:00
abstract::Oxidized cholesterol (oxysterols) plays an important and multifaceted role in lipid metabolism. Here we examined whether dietary oxysterols accelerate hepatic lipid accumulation and inflammation in nonhuman primates. We also examined the effect of the Niemann-Pick C1-like1 inhibitor, ezetimibe (Ez). Macaca fasciculari...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12107
更新日期:2016-09-20 00:00:00
abstract::Dihydroorotate dehydrogenase (DHODH) is a flavin-binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three-dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes poss...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12403
更新日期:2018-03-06 00:00:00
abstract::Adult T-cell leukemia/lymphoma (ATL), an aggressive T-cell malignancy that develops after long-term infection with human T-cell leukemia virus (HTLV-1), requires new treatments. Drug repositioning, reuse of a drug previously approved for the treatment of another condition to treat ATL, offers the possibility of reduce...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12055
更新日期:2016-04-13 00:00:00
abstract::Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function. The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent. Only a few degradat...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12558
更新日期:2018-12-10 00:00:00
abstract::Glutathione transferases (GSTs) are involved in many processes in plant biochemistry, with their best characterised role being the detoxification of xenobiotics through their conjugation with glutathione. GSTs have also been implicated in noncatalytic roles, including the binding and transport of small heterocyclic li...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12168
更新日期:2016-12-22 00:00:00
abstract::Nitrobindins (Nbs), constituting a heme-protein family spanning from bacteria to Homo sapiens, display an all-β-barrel structural organization. Human Nb has been described as a domain of the nuclear protein named THAP4, whose physiological function is still unknown. We report the first evidence of the heme-Fe(III)-bas...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12534
更新日期:2018-11-09 00:00:00
abstract::The A/B domains of nuclear receptors such as thyroid receptor α (TRα) are considered to be conformationally flexible and can potentially adopt multiple structural conformations. We used intrinsic tryptophan fluorescence quenching and circular dichroism spectroscopy to characterize the unfolding of this A/B domain upon...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12229
更新日期:2017-05-15 00:00:00
abstract::Hypoxia-inducible factor (HIF) is a crucial regulator of cellular and systemic responses to low oxygen levels. Here we firstly cloned three HIF-α isoforms from the basal branches of Osteichthyes and used computational tools to characterise the molecular change underlying the functional divergence of HIF-α isoforms in ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.004
更新日期:2013-09-19 00:00:00
abstract::The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12962
更新日期:2020-10-01 00:00:00
abstract::Inorganic polyphosphate (polyP) is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. PolyP promotes osteocalcification and bone mineralization in both mouse and human osteoblastic cells. In the present study, we examined the molecular mechanism by whi...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12703
更新日期:2019-09-01 00:00:00
abstract::Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus mating type and cha...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.07.002
更新日期:2012-07-20 00:00:00
abstract::The Masculinizer (Masc) gene encodes a CCCH-tandem zinc finger protein that controls both masculinization and dosage compensation in the silkworm Bombyx mori. We previously measured the masculinizing activity of the lepidopteran Masc proteins using B. mori ovary-derived cell line BmN-4. Here, we established an RNA-seq...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12698
更新日期:2019-09-01 00:00:00
abstract::Colorectal cancer (CRC) is the third main cause of cancer-relevant deaths worldwide, and its incidence has increased in recent decades. Previous studies have indicated that certain long non-coding RNAs (lncRNAs) have regulatory roles in tumor occurrence and progression. Often, lncRNAs are competitive endogenous RNAs (...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12802
更新日期:2020-01-27 00:00:00
abstract::Niclosamide is a potent inhibitor of osteoclastogenesis and bone remodeling. DK-520 is an acyl derivative of Niclosamide and significantly increased both the plasma concentration and the duration of exposure of Niclosamide when dosed orally. However, at present the effect of DK-520 on osteoclastogenesis has not been r...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12921
更新日期:2020-08-01 00:00:00
abstract::Y-box-binding protein 1 (YB1) is a multifunctional transcription factor with vital roles in proliferation, differentiation and apoptosis. In this study, we have examined the role of its C-terminal domain (YB1 CTD) in proliferation, angiogenesis and tumorigenicity in breast cancer. Breast cancer cell line SK-BR-3 was i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12004
更新日期:2016-01-11 00:00:00
abstract::Insulin signaling plays a central role in the regulation of facilitative glucose transporters (GLUTs) in humans. To establish Caenorhabditis elegans (C. elegans) as a model to study the mechanism underlying insulin regulation of GLUT, we identified that FGT-1 is most likely the only functional GLUT homolog in C. elega...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12068
更新日期:2016-04-21 00:00:00
abstract::A local tissue-specific renin-angiotensin system (local RAS) has been identified in many organs. However, no report has described the role of a local RAS in the hypertrophic differentiation of chondrocytes. To examine the role of a local RAS in the hypertrophic differentiation, we activated angiotensin II type 1 recep...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.07.001
更新日期:2013-07-04 00:00:00
abstract::Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene e...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12386
更新日期:2018-02-19 00:00:00
abstract::Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.05.001
更新日期:2013-05-30 00:00:00
abstract::The yeast cyclins Cln1 and Cln2 are very similar in both sequence and function, but some differences in their functionality and localization have been recently described. The control of Cln1 and Cln2 cellular levels is crucial for proper cell cycle initiation. In this work, we analyzed the degradation patterns of Cln1...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12157
更新日期:2016-12-14 00:00:00
abstract::Increased glucose consumption is a known hallmark of cancer cells. Increased glycolysis provides ATP, reducing agents and substrates for macromolecular synthesis in intensely dividing cells. Therefore, inhibition of glycolysis is one strategy in anticancer therapy as well as in improved efficacy of conventional antica...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12628
更新日期:2019-05-01 00:00:00
abstract::In brain cells, glutamine transporters are vital to monitor and control the levels of glutamate and GABA. There are 11 members of the SLC38 family of amino acid transporters of which eight have been functionally characterized. Here, we report the first histological and functional characterization of the previously orp...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12219
更新日期:2017-04-26 00:00:00