Abstract:
:Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration-dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC-induced cell-cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self-renewal in human pluripotent stem cells.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Han Z,Xu Z,Chen L,Ye D,Yu Y,Zhang Y,Cao Y,Djibril B,Guo X,Gao X,Zhang W,Yu M,Liu S,Yan G,Jin M,Huang Q,Wang X,Hua B,Feng C,Yang F,Ma W,Liu Ydoi
10.1002/2211-5463.12811subject
Has Abstractpub_date
2020-05-01 00:00:00pages
726-733issue
5issn
2211-5463journal_volume
10pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::Arbutin, a glycoside extracted from the plant Arctostaphylos uva-ursi, has been previously reported to possess antioxidant, anti-inflammatory and anticancer effects. Here, we investigated whether arbutin affects the proliferation of the cells of the osteosarcoma (OS) cell lines MG-63 and SW1353. Arbutin suppressed OS ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13024
更新日期:2020-11-03 00:00:00
abstract::Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus mating type and cha...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.07.002
更新日期:2012-07-20 00:00:00
abstract::The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12962
更新日期:2020-10-01 00:00:00
abstract::Hypoxia-inducible factor (HIF) is a crucial regulator of cellular and systemic responses to low oxygen levels. Here we firstly cloned three HIF-α isoforms from the basal branches of Osteichthyes and used computational tools to characterise the molecular change underlying the functional divergence of HIF-α isoforms in ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.004
更新日期:2013-09-19 00:00:00
abstract::The yeast cyclins Cln1 and Cln2 are very similar in both sequence and function, but some differences in their functionality and localization have been recently described. The control of Cln1 and Cln2 cellular levels is crucial for proper cell cycle initiation. In this work, we analyzed the degradation patterns of Cln1...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12157
更新日期:2016-12-14 00:00:00
abstract::Lactobionic acid (O-β-galactosyl-(1-4)-gluconic acid) (LBA) is a high-value lactose derivative, produced via oxidation of the reducing terminal of lactose. LBA can be produced by fermentation using certain microorganisms, although subsequent purification is challenging. Therefore, we have attempted to identify an enzy...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12582
更新日期:2019-01-16 00:00:00
abstract::Centrins are members of the EF-hand family of calcium-binding proteins, which are highly conserved among eukaryotes. Centrins bind to several cellular targets, through a hydrophobic triad. However, the W(1)xxL(4)xxxL(8) triad in XPC (Xeroderma Pigmentosum Group C protein) is found in the reverse orientation, as in the...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.11.005
更新日期:2013-12-05 00:00:00
abstract::The proton-coupled folate transporter (PCFT, SLC46A1) transports folic acid across the plasma membrane, together with an excess of protons such that the net charge translocation is positive. We developed 3D structural models of PCFT threaded onto the X-ray structures of major facilitator superfamily (MFS) members that...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12041
更新日期:2016-02-22 00:00:00
abstract::Dedifferentiation of adipocytes contributes to the generation of a proliferative cell population that could be useful in cellular therapy or tissue engineering. Adipocytes can dedifferentiate into precursor cells to acquire a fibroblast-like phenotype using ceiling culture, in which the buoyancy of fat cells is exploi...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12250
更新日期:2017-07-10 00:00:00
abstract::Oxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate c...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.05.001
更新日期:2012-05-30 00:00:00
abstract::In this study, we showed that cyanocobalamin dodecylamine, a ribose 5'-carbamate derivative of cyanocobalamin, was absorbed and accumulated to significant levels by Caenorhabditis elegans and was not further metabolized. The levels of methylmalonic acid and homocysteine, which serve as indicators of cobalamin deficien...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.07.008
更新日期:2014-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infection can be accelerated by intracellular and extracellular hydrogen peroxide (H2O2) stimulation, mediated by the activation of the p38 mitogen-activated protein kinase (MAPK) pathway. However, it remains unknown whether host gene expression is involved in H2O2-upregulated HCMV replica...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12105
更新日期:2016-08-15 00:00:00
abstract::Shiga toxin (Stx) causes fatal systemic complications. Stx induces apoptosis, but the mechanism of which is unclear. We report that Stx induced rapid reduction of short-lived anti-apoptotic proteins followed by activation of caspase 9 and the progression of apoptosis. Proteasome inhibitors prevented the reduction of a...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.005
更新日期:2015-06-12 00:00:00
abstract::Recent evidence has shown that miR-409-3p was down-regulated in several types of cancer, including osteosarcoma. However, the potential role of miR-409-3p in osteosarcoma remains largely unknown. In the present study, we showed that overexpression of miR-409-3p in osteosarcoma cells inhibited cell proliferation in vit...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12177
更新日期:2017-01-27 00:00:00
abstract::Microorganisms or LPS (lipopolysaccharide), an outer membrane component of Gram-negative bacteria, can induce a systemic inflammatory response that leads to sepsis, multiple organ dysfunction, and mortality. Here, we investigated the role of cyclophilin D (CypD)-dependent mitochondrial permeability transition (mPT) in...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13091
更新日期:2021-01-20 00:00:00
abstract::The identification of non-invasive biomarkers for the detection of renal cell carcinoma (RCC) in early-stage patients may help improve disease outcome. Certain long non-coding RNAs (lncRNAs) have been reported to be possible biomarkers for the diagnosis and prognosis of cancer. Here, we examined the suitability of the...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12930
更新日期:2020-09-01 00:00:00
abstract::Proteins of the serum amyloid A (SAA) family have been remarkably conserved in evolution. Their biologic function(s) are not fully defined but they are likely to be a part of primordial host defense. We have detected a ∼ 12-kDa protein reacting with antibodies against serum amyloid A (SAA) in human colostrum by wester...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12383
更新日期:2018-01-31 00:00:00
abstract::Goji berries have been used as food and medicine for millennia. Due to their high morphological similarity, fruits of two distinct species belonging to the family Solanaceae, Lycium barbarum (LB) and Lycium chinense (Chinese boxthorn), are usually marketed together as goji berries, but nearly 90% of all commercially a...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12910
更新日期:2020-08-01 00:00:00
abstract::Cisplatin-based chemotherapy is the standard treatment of choice for head and neck squamous cell carcinoma (HNSCC). The efficiency of platinum-based therapies is directly influenced by the development of tumor resistance. Multiple signaling pathways have been linked to tumor resistance, including activation of nuclear...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.12.003
更新日期:2013-12-30 00:00:00
abstract::Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function. The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent. Only a few degradat...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12558
更新日期:2018-12-10 00:00:00
abstract::Hsp90 is essential for maintaining the activity of numerous signaling factors, and plays a key role in cellular signal transduction networks. 17-Allylamino-17-demethoxygeldanamycin (17-AAG) is an ansamycin antibiotic that binds to Hsp90 and inhibits its function. HaCaT human keratinocytes were used to investigate the ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.03.002
更新日期:2012-03-25 00:00:00
abstract::DNA damage-inducible 1 (Ddi1) is a multidomain protein with one of the domains being retropepsin-like. HIV-1 protease inhibitors were found to reduce opportunistic infections caused by pathogens like Leishmania and Plasmodium, and some of them were shown to inhibit the growth of these parasites. In Leishmania, Ddi1 wa...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12491
更新日期:2018-08-03 00:00:00
abstract::Despite significant advances in understanding of the causes of and treatment of myocardial infarction (MI) in recent years, morbidity and mortality is still high. The aim of this study was to identify miRNA and genes potentially associated with MI. mRNA and miRNA expression datasets were downloaded from the Gene Expre...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12423
更新日期:2018-04-19 00:00:00
abstract::Peroxisome proliferator-activated receptor-gamma coactivator-1β (PGC-1β) is a transcriptional regulator whose increased expression activates energy expenditure-related genes in skeletal muscles. However, how PGC-1β is regulated remains largely unclear. Here, we show that PGC-1β gene expression is negatively correlated...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12898
更新日期:2020-07-01 00:00:00
abstract::l-Ornithine cyclodeaminase (OCD) is involved in l-proline biosynthesis and catalyzes the unique deaminating cyclization of l-ornithine to l-proline via a Δ(1)-pyrroline-2-carboxyrate (Pyr2C) intermediate. Although this pathway functions in only a few bacteria, many archaea possess OCD-like genes (proteins), among whic...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.07.005
更新日期:2014-07-10 00:00:00
abstract::Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is an important enzyme in phospholipid remodeling, a process that influences the biophysical properties of cell membranes and thus cell function. Multiple lines of evidence suggest that LPCAT3 is involved in several diseases, including atherosclerosis, non-alcoholic s...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12712
更新日期:2019-10-01 00:00:00
abstract::Epigallocatechin gallate (EGCG), a major polyphenol in green tea, possesses antioxidant properties and regulates various cell functions. Here, we examined the function of EGCG in inflammatory bone resorption. In calvarial organ cultures, lipopolysaccharide (LPS)-induced bone resorption was clearly suppressed by EGCG. ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.003
更新日期:2015-06-12 00:00:00
abstract::Understanding the role of specific amino acid residues in the molecular mechanism of a protein's function is one of the most challenging problems in modern biology. A systematic bioinformatic analysis of protein families and superfamilies can help in the study of structure-function relationships and in the design of i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12441
更新日期:2018-05-21 00:00:00
abstract::IL-1 family cytokines act as apical initiators of inflammation in many settings and can promote the production of a battery of inflammatory cytokines, chemokines and other inflammatory mediators in diverse cell types. IL-36α, IL-36β and IL-36γ, which belong to the extended IL-1 family, have been implicated as key init...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12406
更新日期:2018-03-25 00:00:00
abstract::Oxidative stress in plants causes ferredoxin down-regulation and NADP(+) shortage, over-reduction of the photosynthetic electron transport chain, electron leakage to oxygen and generation of reactive oxygen species (ROS). Expression of cyanobacterial flavodoxin in tobacco chloroplasts compensates for ferredoxin declin...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2011.10.004
更新日期:2011-11-11 00:00:00