Iron overload inhibits self-renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species.


:Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration-dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC-induced cell-cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self-renewal in human pluripotent stem cells.


FEBS Open Bio


FEBS open bio


Han Z,Xu Z,Chen L,Ye D,Yu Y,Zhang Y,Cao Y,Djibril B,Guo X,Gao X,Zhang W,Yu M,Liu S,Yan G,Jin M,Huang Q,Wang X,Hua B,Feng C,Yang F,Ma W,Liu Y




Has Abstract


2020-05-01 00:00:00










  • Potential role of platelets for atherosclerotic events in rheumatoid arthritis.

    abstract::Rheumatoid arthritis (RA) is a chronic inflammatory disease with increased risk of cardiovascular events and mortality that can be attributed to accelerated atherosclerosis. This pilot study aimed to investigate if changes in blood parameters were compatible with atherosclerotic events in RA patients. To this aim, 45 ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Vona R,Di Franco M,Gambardella L,Di Lollo AC,Iannuccelli C,Valesini G,Malorni W,Straface E

    更新日期:2018-11-06 00:00:00

  • Activating types 1 and 2 angiotensin II receptors modulate the hypertrophic differentiation of chondrocytes.

    abstract::A local tissue-specific renin-angiotensin system (local RAS) has been identified in many organs. However, no report has described the role of a local RAS in the hypertrophic differentiation of chondrocytes. To examine the role of a local RAS in the hypertrophic differentiation, we activated angiotensin II type 1 recep...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Tsukamoto I,Inoue S,Teramura T,Takehara T,Ohtani K,Akagi M

    更新日期:2013-07-04 00:00:00

  • Cellular calcification induced by inorganic polyphosphate involves ATP depletion and opening of the mitochondrial permeability transition pore (mPTP).

    abstract::Inorganic polyphosphate (polyP) is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. PolyP promotes osteocalcification and bone mineralization in both mouse and human osteoblastic cells. In the present study, we examined the molecular mechanism by whi...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Tsutsumi K,Sasase T

    更新日期:2019-09-01 00:00:00

  • Mutational and crystallographic analysis of l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities.

    abstract::In this study, it was shown for the first time that l-amino acid oxidase of Pseudomonas sp. AIU813, renamed as l-amino acid oxidase/monooxygenase (l-AAO/MOG), exhibits l-lysine 2-monooxygenase as well as oxidase activity. l-Lysine oxidase activity of l-AAO/MOG was increased in a p-chloromercuribenzoate (p-CMB) concent...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Matsui D,Im DH,Sugawara A,Fukuta Y,Fushinobu S,Isobe K,Asano Y

    更新日期:2014-02-07 00:00:00

  • Expression patterns of kiss2 and gpr54-2 in Monopterus albus suggest these genes may play a role in sex reversal in fish.

    abstract::Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals,...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Yi TL,Pei MT,Yang DQ

    更新日期:2019-10-01 00:00:00

  • Interaction of Carthamus tinctorius lignan arctigenin with the binding site of tryptophan-degrading enzyme indoleamine 2,3-dioxygenase.

    abstract::Mediterranean Carthamus tinctorius (Safflower) is used for treatment of inflammatory conditions and neuropsychiatric disorders. Recently C. tinctorius lignans arctigenin and trachelogenin but not matairesinol were described to interfere with the activity of tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO)...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Temml V,Kuehnl S,Schuster D,Schwaiger S,Stuppner H,Fuchs D

    更新日期:2013-09-07 00:00:00

  • Human nitrobindin: the first example of an all-β-barrel ferric heme-protein that catalyzes peroxynitrite detoxification.

    abstract::Nitrobindins (Nbs), constituting a heme-protein family spanning from bacteria to Homo sapiens, display an all-β-barrel structural organization. Human Nb has been described as a domain of the nuclear protein named THAP4, whose physiological function is still unknown. We report the first evidence of the heme-Fe(III)-bas...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: De Simone G,di Masi A,Polticelli F,Ascenzi P

    更新日期:2018-11-09 00:00:00

  • A cataract-causing connexin 50 mutant is mislocalized to the ER due to loss of the fourth transmembrane domain and cytoplasmic domain.

    abstract::Mutations in the eye lens gap junction protein connexin 50 cause cataract. Earlier we identified a frameshift mutant of connexin 50 (c.670insA; p.Thr203AsnfsX47) in a family with autosomal recessive cataract. The mutant protein is smaller and contains 46 aberrant amino acids at the C-terminus after amino acid 202. Her...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Somaraju Chalasani ML,Muppirala M,G Ponnam SP,Kannabiran C,Swarup G

    更新日期:2012-11-27 00:00:00

  • SPC25 may promote proliferation and metastasis of hepatocellular carcinoma via p53.

    abstract::Hepatocellular carcinoma (HCC) is a common malignancy with poor prognosis and high mortality. To identify key genes associated with HCC and the underlying mechanisms, we performed weighted correlation network analysis (WGCNA) of potential key genes of HCC. We identified 17 key genes closely related to HCC by yellow mo...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Chen F,Zhang K,Huang Y,Luo F,Hu K,Cai Q

    更新日期:2020-07-01 00:00:00

  • Fruit ripening in Lycium barbarum and Lycium ruthenicum is associated with distinct gene expression patterns.

    abstract::Goji berries have been used as food and medicine for millennia. Due to their high morphological similarity, fruits of two distinct species belonging to the family Solanaceae, Lycium barbarum (LB) and Lycium chinense (Chinese boxthorn), are usually marketed together as goji berries, but nearly 90% of all commercially a...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Zhao J,Li H,Yin Y,An W,Qin X,Wang Y,Fan Y,Li Y,Cao Y

    更新日期:2020-08-01 00:00:00

  • Transcriptome analysis of roots from resistant and susceptible rice varieties infected with Hirschmanniella mucronata.

    abstract::Hirschmanniella mucronata is a plant-parasitic nematode that is widespread in rice production areas and causes 10-25% yield losses a year on average. Here, we investigated the mechanism of resistance to this nematode by comparing the transcriptomes of roots from resistant (Jiabali) and susceptible (Bawangbian) varieti...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Sun X,Zhang L,Tang Z,Shi X,Ma J,Cui R

    更新日期:2019-11-01 00:00:00

  • Regulation of Neisseria meningitidis cytochrome bc1 components by NrrF, a Fur-controlled small noncoding RNA.

    abstract::NrrF is a small regulatory RNA of the human pathogen Neisseria meningitidis. NrrF was previously shown to repress succinate dehydrogenase (sdhCDAB) under control of the ferric uptake regulator (Fur). Here, we provide evidence that cytochrome bc 1 , encoded by the polycistro...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Pannekoek Y,Huis In 't Veld R,Schipper K,Bovenkerk S,Kramer G,Speijer D,van der Ende A

    更新日期:2017-08-05 00:00:00

  • Cyclophilin D-dependent mitochondrial permeability transition amplifies inflammatory reprogramming in endotoxemia.

    abstract::Microorganisms or LPS (lipopolysaccharide), an outer membrane component of Gram-negative bacteria, can induce a systemic inflammatory response that leads to sepsis, multiple organ dysfunction, and mortality. Here, we investigated the role of cyclophilin D (CypD)-dependent mitochondrial permeability transition (mPT) in...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Veres B,Eros K,Antus C,Kalman N,Fonai F,Jakus PB,Boros E,Hegedus Z,Nagy I,Tretter L,Gallyas F Jr,Sumegi B

    更新日期:2021-01-20 00:00:00

  • Characterisation of a novel cold-adapted calcium-activated transglutaminase: implications for medicine and food processing.

    abstract::Transglutaminases are a family of enzymes that catalyse the cross-linking of proteins by forming covalent bonds between lysine and glutamine residues in various polypeptides. Cross-linking reactions are involved in blood clots, skin formation, embryogenesis and apoptosis. Clinically, these enzymes appear to be implica...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Alvarez RG,Karki P,Langleite IE,Bakksjø RJ,Eichacker LA,Furnes C

    更新日期:2020-04-01 00:00:00

  • Structure of the C-terminal domain of AspA (antigen I/II-family) protein from Streptococcus pyogenes.

    abstract::The pathogenic bacteria Streptococcus pyogenes can cause an array of diseases in humans, including moderate infections such as pharyngitis (strep throat) as well as life threatening conditions such as necrotizing fasciitis and puerperal fever. The antigen I/II family proteins are cell wall anchored adhesin proteins fo...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Hall M,Nylander S,Jenkinson HF,Persson K

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  • Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri, the causative agent of citrus canker disease.

    abstract::Inovirus XacF1 (7325 nucleotides) is integrated into the genome of Xanthomonas citri pv. citri (Xcc) strains at the host dif site (attB) by the host XerC/D recombination system. The XacF1 attP sequence is located within the coding region of ORF12, a possible phage regulator. After integration, this open reading frame ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Ahmad AA,Kawabe M,Askora A,Kawasaki T,Fujie M,Yamada T

    更新日期:2017-09-19 00:00:00

  • Hypoxia affects Slc7a5 expression through HIF-2α in differentiated neuronal cells.

    abstract::An imbalance of branched-chain amino acids (BCAAs) in the brain may result in neuropathological conditions, such as autism spectrum disorders. The L-type amino acid transporter 1 (LAT1), encoded by the solute carrier transporter 7a5 (Slc7a5) gene, is critical for maintaining normal levels of BCAAs in the brain. Howeve...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Onishi Y,Hiraiwa M,Kamada H,Iezaki T,Yamada T,Kaneda K,Hinoi E

    更新日期:2019-01-07 00:00:00

  • Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene.

    abstract::Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus mating type and cha...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Karimi S,Wetzel J,Wöstemeyer J,Burmester A

    更新日期:2012-07-20 00:00:00

  • Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC.

    abstract::Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Khusainov R,Moll GN,Kuipers OP

    更新日期:2013-05-30 00:00:00

  • Bioinformatic analysis of the fold type I PLP-dependent enzymes reveals determinants of reaction specificity in l-threonine aldolase from Aeromonas jandaei.

    abstract::Understanding the role of specific amino acid residues in the molecular mechanism of a protein's function is one of the most challenging problems in modern biology. A systematic bioinformatic analysis of protein families and superfamilies can help in the study of structure-function relationships and in the design of i...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Fesko K,Suplatov D,Švedas V

    更新日期:2018-05-21 00:00:00

  • Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase.

    abstract::Oxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate c...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Freitas M,Baldeiras I,Proença T,Alves V,Mota-Pinto A,Sarmento-Ribeiro A

    更新日期:2012-05-30 00:00:00

  • The gene expression of numerous SLC transporters is altered in the immortalized hypothalamic cell line N25/2 following amino acid starvation.

    abstract::Amino acids are known to play a key role in gene expression regulation, and in mammalian cells, amino acid signaling is mainly mediated via two pathways, the mammalian target of rapamycin complex 1 (mTORC1) pathway and the amino acid responsive (AAR) pathway. It is vital for cells to have a system to sense amino acid ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Hellsten SV,Lekholm E,Ahmad T,Fredriksson R

    更新日期:2017-01-11 00:00:00

  • Deregulation of miR-27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line.

    abstract::The tumour microenvironment comprises a diverse range of cells, including fibroblasts, immune cells and endothelial cells, along with extracellular matrix. In particular, fibroblasts are of significant interest as these cells are reprogrammed during tumorigenesis to become cancer-associated fibroblasts (CAFs), which i...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Aguilera-Rojas M,Sharbati S,Stein T,Einspanier R

    更新日期:2020-05-01 00:00:00

  • Death receptor 6 promotes ovarian cancer cell migration through KIF11.

    abstract::The expression of death receptor 6 (DR6) is abnormal in some cancer types, but the function and underlying molecular mechanisms of DR6 in tumor progression are not yet clear. In the present study, our analysis of ovarian cancer RNA sequencing data from The Cancer Genome Atlas revealed that DR6 is upregulated in human ...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Shi B,Bao J,Liu Y,Shi J

    更新日期:2018-08-07 00:00:00

  • Niclosamide and its derivative DK-520 inhibit RANKL-induced osteoclastogenesis.

    abstract::Niclosamide is a potent inhibitor of osteoclastogenesis and bone remodeling. DK-520 is an acyl derivative of Niclosamide and significantly increased both the plasma concentration and the duration of exposure of Niclosamide when dosed orally. However, at present the effect of DK-520 on osteoclastogenesis has not been r...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Jiao Y,Chen C,Hu X,Feng X,Shi Z,Cao J,Li Q,Zhu Y

    更新日期:2020-08-01 00:00:00

  • Epigenetic regulation of regulatory T cells in patients with abdominal aortic aneurysm.

    abstract::Abdominal arterial aneurysm (AAA) shares many features with autoimmune diseases and appears to be a T-cell-mediated process. In addition, certain epigenetic changes, including DNA methylation, are associated with AAA. In this study, we investigated epigenetic modifications in regulatory T cells (Tregs) from AAA patien...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Xia Q,Zhang J,Han Y,Zhang X,Jiang H,Lun Y,Wu X,Gang Q,Liu Z,Böckler D,Duan Z,Xin S

    更新日期:2019-06-01 00:00:00

  • The clinical value of miR-193a-3p in non-small cell lung cancer and its potential molecular mechanism explored in silico using RNA-sequencing and microarray data.

    abstract::miR-193a-3p is a tumor-related miRNA playing an essential role in tumorigenesis and progression of non-small cell lung cancer (NSCLC). The objective of the present study was to investigate the relationship between miR-193a-3p expression and clinical value and to further explore the potential signaling of miR-193a-3p i...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Gao X,Tang RX,Xie QN,Lin JY,Shi HL,Chen G,Li ZY

    更新日期:2018-01-04 00:00:00

  • Δ12 fatty acid desaturase gene from Geotrichum candidum in cheese: molecular cloning and functional characterization.

    abstract::Soft cheese with white rind lacks essential fatty acids (EFAs), and as a result its long-term consumption may lead to various kinds of cardiovascular and cerebrovascular diseases, such as hyperlipidemia, hypertension, and atherosclerosis. Geotrichum candidum is a dimorphic yeast that plays an important role in the rip...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Luo X,Shi H,Wu R,Wu J,Pi Y,Zheng Y,Yue X

    更新日期:2018-12-06 00:00:00

  • Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing.

    abstract::One practical application of genome-scale metabolic reconstructions is to interrogate multispecies relationships. Here, we report a consensus metabolic model in four yeast species (Saccharomyces cerevisiae, S. paradoxus, S. mikatae, and S. bayanus) by integrating metabolic network simulations with RNA sequencing (RNA-...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Zhao Y,Wang Y,Zou L,Huang J

    更新日期:2016-02-27 00:00:00

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    abstract::Neointimal hyperplasia (NIH) is a complicated inflammatory process contributing to vascular restenosis. The present study aimed to explore whether chemokine-like factor 1 (CKLF1) aggravates NIH via the nuclear factor-kappa B (NF-κB)/vascular cell adhesion molecule-1 (VCAM-1) pathway. We found the expression of CKLF1 a...

    journal_title:FEBS open bio

    pub_type: 杂志文章


    authors: Liu X,Qu C,Zhang Y,Fang J,Teng L,Zhang R,Zhang X,Shen C

    更新日期:2020-09-01 00:00:00