Iron overload inhibits self-renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species.

Abstract:

:Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration-dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC-induced cell-cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self-renewal in human pluripotent stem cells.

journal_name

FEBS Open Bio

journal_title

FEBS open bio

authors

Han Z,Xu Z,Chen L,Ye D,Yu Y,Zhang Y,Cao Y,Djibril B,Guo X,Gao X,Zhang W,Yu M,Liu S,Yan G,Jin M,Huang Q,Wang X,Hua B,Feng C,Yang F,Ma W,Liu Y

doi

10.1002/2211-5463.12811

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

726-733

issue

5

issn

2211-5463

journal_volume

10

pub_type

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