Abstract:
:Liver cancer stem cells (L-CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long-term culture model for a specific subpopulation of L-CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L-CSCs, which we then cultured in modified chemically defined medium. The enriched L-CSCs exhibited enhanced proliferation, self-renewal and long-term clonal maintenance ability as compared with non-CSCs. Compared with wild-type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L-CSCs was significantly increased. In summary, the subpopulation of L-CSCs still maintains cancer stem cell-related phenotypes after 14 days of culture.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Zhang B,Wang HY,Wang DX,Zeng Q,Fan Z,Xi JF,Nan X,He LJ,Zhou JN,Pei XT,Yue Wdoi
10.1002/2211-5463.12932subject
Has Abstractpub_date
2020-09-01 00:00:00pages
1737-1747issue
9issn
2211-5463journal_volume
10pub_type
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