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eLife文献大全abstract::Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54449
更新日期:2019-12-30 00:00:00
abstract::Cells use molecular circuits to interpret and respond to extracellular cues, such as hormones and cytokines, which are often released in a temporally varying fashion. In this study, we combine microfluidics, time-lapse microscopy, and computational modeling to investigate how the type I interferon (IFN)-responsive reg...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58825
更新日期:2020-09-18 00:00:00
abstract::Layer (L) 2/3 pyramidal neurons in the primary somatosensory cortex (S1) are sparsely active, spontaneously and during sensory stimulation. Long-range inputs from higher areas may gate L2/3 activity. We investigated their in vivo impact by expressing channelrhodopsin in three main sources of feedback to rat S1: primar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44158
更新日期:2019-02-11 00:00:00
abstract::Class IV ddaC neurons specifically prune larval dendrites without affecting axons during Drosophila metamorphosis. ddaCs distribute the minus ends of microtubules (MTs) to dendrites but the plus ends to axons. However, a requirement of MT minus-end-binding proteins in dendrite-specific pruning remains completely unkno...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39964
更新日期:2019-03-28 00:00:00
abstract::Ligand binding stabilizes different G protein-coupled receptor states via a complex allosteric process that is not completely understood. Here, we have derived free energy landscapes describing activation of the β2 adrenergic receptor bound to ligands with different efficacy profiles using enhanced sampling molecular ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60715
更新日期:2021-01-28 00:00:00
abstract::In most animals, the brain controls the body via a set of descending neurons (DNs) that traverse the neck. DN activity activates, maintains or modulates locomotion and other behaviors. Individual DNs have been well-studied in species from insects to primates, but little is known about overall connectivity patterns acr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34272
更新日期:2018-06-26 00:00:00
abstract::Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35621
更新日期:2018-06-25 00:00:00
abstract::INAD assembles key enzymes of the Drosophila compound eye photo-transduction pathway into a supramolecular complex, supporting efficient and fast light signaling. However, the molecular mechanism that governs the interaction between INAD and NORPA (phospholipase Cβ, PLCβ), a key step for the fast kinetics of the light...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41848
更新日期:2018-12-10 00:00:00
abstract::Despite employing diverse molecular mechanisms, many different cell signaling systems avoid losing information by transmitting it in a linear manner. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.41894
更新日期:2018-10-25 00:00:00
abstract::The growth of preprints in the life sciences has been reported widely and is driving policy changes for journals and funders, but little quantitative information has been published about preprint usage. Here, we report how we collected and analyzed data on all 37,648 preprints uploaded to bioRxiv.org, the largest biol...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45133
更新日期:2019-04-24 00:00:00
abstract::The mesiotemporal lobe (MTL) is implicated in many cognitive processes, is compromised in numerous brain disorders, and exhibits a gradual cytoarchitectural transition from six-layered parahippocampal isocortex to three-layered hippocampal allocortex. Leveraging an ultra-high-resolution histological reconstruction of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60673
更新日期:2020-11-04 00:00:00
abstract::Lissencephaly ('smooth brain') is a severe brain disease associated with numerous symptoms, including cognitive impairment, and shortened lifespan. The main causative gene of this disease - lissencephaly-1 (LIS1) - has been a focus of intense scrutiny since its first identification almost 30 years ago. LIS1 is a criti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59737
更新日期:2020-07-21 00:00:00
abstract::Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell commun...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50598
更新日期:2019-10-02 00:00:00
abstract::Major depressive disorder (MDD) is a prevalent illness that can be precipitated by acute or chronic stress. Studies of patients with Wolfram syndrome and carriers have identified Wfs1 mutations as causative for MDD. The medial prefrontal cortex (mPFC) is known to be involved in depression and behavioral resilience, al...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08752
更新日期:2015-09-15 00:00:00
abstract::With implantation, mouse stromal cells begin to transform into epithelial-like cells surrounding the implantation chamber forming an avascular zone called the primary decidual zone (PDZ). In the mouse, the PDZ forms a transient, size-dependent permeable barrier to protect the embryo from maternal circulating harmful a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61762
更新日期:2020-09-29 00:00:00
abstract::Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Oppos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58880
更新日期:2020-07-06 00:00:00
abstract::During embryogenesis, chromatin accessibility profiles control lineage-specific gene expression by modulating transcription, thus impacting multipotent progenitor states and subsequent fate choices. Subsets of cardiac and pharyngeal/head muscles share a common origin in the cardiopharyngeal mesoderm, but the chromatin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49921
更新日期:2019-11-20 00:00:00
abstract::Eliminating malaria from a defined region involves draining the endemic parasite reservoir and minimizing local malaria transmission around imported malaria infections . In the last phases of malaria elimination, as universal interventions reap diminishing marginal returns, national resources must become increasingly ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09520
更新日期:2015-12-29 00:00:00
abstract::IRE1α is an endoplasmic reticulum (ER) localized endonuclease activated by misfolded proteins in the ER. Previously, we demonstrated that IRE1α forms a complex with the Sec61 translocon, to which its substrate XBP1u mRNA is recruited for cleavage during ER stress (Plumb et al., 2015). Here, we probe IRE1α complexes in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27187
更新日期:2017-05-15 00:00:00
abstract::Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07735
更新日期:2015-08-19 00:00:00
abstract::Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53944
更新日期:2020-01-20 00:00:00
abstract::Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the cell. Mechanical forces are known to play a major role in plant cell shape by controlling ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57282
更新日期:2020-09-01 00:00:00
abstract::The two centrioles of the centrosome in quiescent cells are inherently asymmetric structures that differ in age, morphology and function. How these asymmetric properties are established and maintained during quiescence remains unknown. Here, we show that a daughter centriole-associated ciliopathy protein, Cep120, play...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35439
更新日期:2018-05-09 00:00:00
abstract::Previously we identified Lam/GramD1 proteins, a family of endoplasmic reticulum membrane proteins with sterol-binding StARkin domains that are implicated in intracellular sterol homeostasis. Here, we show how these proteins exchange sterol molecules with membranes. An aperture at one end of the StARkin domain enables ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53444
更新日期:2019-12-04 00:00:00
abstract::Microbes are embedded in complex communities where they engage in a wide array of intra- and inter-specific interactions. The extent to which these interactions drive or impede microbiome diversity is not well understood. Historically, two contrasting hypotheses have been suggested to explain how species interactions ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58999
更新日期:2020-11-20 00:00:00
abstract::The non-canonical Wnt/Ca2+ signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that the zebrafish/human phosphatidylinosit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26362
更新日期:2017-05-02 00:00:00
abstract::Tumor-initiating stem cells (SCs) exhibit distinct patterns of transcription factors and gene expression compared to healthy counterparts. Here, we show that dramatic shifts in large open-chromatin domain (super-enhancer) landscapes underlie these differences and reflect tumor microenvironment. By in vivo super-enhanc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10870
更新日期:2015-11-21 00:00:00
abstract::Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. The Hippo kinase cascade represses the growth-promoting transcription co-activator Yorkie. The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46592
更新日期:2019-09-30 00:00:00
abstract::Modern neuroscience research often requires the coordination of multiple processes such as stimulus generation, real-time experimental control, as well as behavioral and neural measurements. The technical demands required to simultaneously manage these processes with high temporal fidelity is a barrier that limits the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40231
更新日期:2019-02-07 00:00:00
abstract::The TP53 tumor-suppressor gene is mutated in >50% of human tumors and Li-Fraumeni patients with germ line inactivation are predisposed to developing cancer. Here, we generated tp53 deleted zebrafish that spontaneously develop malignant peripheral nerve-sheath tumors, angiosarcomas, germ cell tumors, and an aggressive ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37202
更新日期:2018-09-07 00:00:00