Abstract:
:Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored to this core by the N-terminus of Sth1. Our cryo-EM analysis of RSC bound to a nucleosome core particle shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of the Rsc8 SWIRM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.
journal_name
Elifejournal_title
eLifeauthors
Patel AB,Moore CM,Greber BJ,Luo J,Zukin SA,Ranish J,Nogales Edoi
10.7554/eLife.54449subject
Has Abstractpub_date
2019-12-30 00:00:00issn
2050-084Xpii
54449journal_volume
8pub_type
杂志文章相关文献
eLife文献大全abstract::After paralyzing spinal cord injury the adult nervous system has little ability to 'heal' spinal connections, and it is assumed to be unable to develop extra-spinal recovery strategies to bypass the lesion. We challenge this assumption, showing that completely spinalized adult rats can recover unassisted hindlimb weig...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23532
更新日期:2017-06-29 00:00:00
abstract::Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00230
更新日期:2013-01-22 00:00:00
abstract::The Maternal Embryonic Leucine Zipper Kinase (MELK) has been identified as a promising therapeutic target in multiple cancer types. MELK over-expression is associated with aggressive disease, and MELK has been implicated in numerous cancer-related processes, including chemotherapy resistance, stem cell renewal, and tu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32838
更新日期:2018-02-08 00:00:00
abstract::Behavior relies on the ability of sensory systems to infer properties of the environment from incoming stimuli. The accuracy of inference depends on the fidelity with which behaviorally relevant properties of stimuli are encoded in neural responses. High-fidelity encodings can be metabolically costly, but low-fidelity...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32055
更新日期:2018-07-10 00:00:00
abstract::The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06866
更新日期:2015-07-29 00:00:00
abstract::Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49734
更新日期:2020-02-07 00:00:00
abstract::Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51461
更新日期:2020-01-17 00:00:00
abstract::Ligand binding stabilizes different G protein-coupled receptor states via a complex allosteric process that is not completely understood. Here, we have derived free energy landscapes describing activation of the β2 adrenergic receptor bound to ligands with different efficacy profiles using enhanced sampling molecular ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60715
更新日期:2021-01-28 00:00:00
abstract::Rapid diagnostic tests (RDTs) have transformed malaria diagnosis. The most prevalent P. falciparum RDTs detect histidine-rich protein 2 (PfHRP2). However, pfhrp2 gene deletions yielding false-negative RDTs, first reported in South America in 2010, have been confirmed in Africa and Asia. We developed a mathematical mod...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25008
更新日期:2017-08-24 00:00:00
abstract::Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37550
更新日期:2018-11-01 00:00:00
abstract::While specific mutations allow organisms to adapt to stressful environments, most changes in an organism's DNA negatively impact fitness. The mutation rate is therefore strictly regulated and often considered a slowly-evolving parameter. In contrast, we demonstrate an unexpected flexibility in cellular mutation rates ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22939
更新日期:2017-05-02 00:00:00
abstract::Transport of biologically active molecules across tight epithelial barriers is a major challenge preventing therapeutic peptides from oral drug delivery. Here, we identify a set of synthetic glycosphingolipids that harness the endogenous process of intracellular lipid-sorting to enable mucosal absorption of the incret...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34469
更新日期:2018-05-31 00:00:00
abstract::Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00533
更新日期:2013-09-10 00:00:00
abstract::A transcription factor that regulates skeleton formation in sea urchin embryos has evolved a new domain that is essential for this process. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.33792
更新日期:2018-01-08 00:00:00
abstract::Rats exhibit 'empathy' making them a model to understand the neural underpinnings of such behavior. We show data consistent with these findings, but also that behavior and dopamine (DA) release reflects subjective rather than objective evaluation of appetitive and aversive events that occur to another. We recorded DA ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38090
更新日期:2018-11-28 00:00:00
abstract::Retromer, including Vps35, Vps26, and Vps29, is a protein complex responsible for recycling proteins within the endolysosomal pathway. Although implicated in both Parkinson's and Alzheimer's disease, our understanding of retromer function in the adult brain remains limited, in part because Vps35 and Vps26 are essentia...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51977
更新日期:2020-04-14 00:00:00
abstract::Motor fatigability emerges when demanding tasks are executed over an extended period of time. Here, we used repetitive low-force movements that cause a gradual reduction in movement speed (or 'motor slowing') to study the central component of fatigability in healthy adults. We show that motor slowing is associated wit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46750
更新日期:2019-09-16 00:00:00
abstract::Adhesion molecules hold cells together but also couple cell membranes to a contractile actomyosin network, which limits the expansion of cell contacts. Despite their fundamental role in tissue morphogenesis and tissue homeostasis, how adhesion molecules control cell shapes and cell patterns in tissues remains unclear....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22796
更新日期:2017-05-24 00:00:00
abstract::Aeromonas bacteria living in the gut of zebrafish produce a specific molecule to pacify the immune system of their host. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.42676
更新日期:2018-11-06 00:00:00
abstract::Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20983
更新日期:2017-01-10 00:00:00
abstract::Class IV ddaC neurons specifically prune larval dendrites without affecting axons during Drosophila metamorphosis. ddaCs distribute the minus ends of microtubules (MTs) to dendrites but the plus ends to axons. However, a requirement of MT minus-end-binding proteins in dendrite-specific pruning remains completely unkno...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39964
更新日期:2019-03-28 00:00:00
abstract::The non-canonical Wnt/Ca2+ signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that the zebrafish/human phosphatidylinosit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26362
更新日期:2017-05-02 00:00:00
abstract::Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haph...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55308
更新日期:2020-09-21 00:00:00
abstract::The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R blockade was a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55564
更新日期:2020-04-01 00:00:00
abstract::Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59614
更新日期:2020-10-06 00:00:00
abstract::In complex biological systems, simple individual-level behavioral rules can give rise to emergent group-level behavior. While collective behavior has been well studied in cells and larger organisms, the mesoscopic scale is less understood, as it is unclear which sensory inputs and physical processes matter a priori. H...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43318
更新日期:2019-04-25 00:00:00
abstract::Most cellular features have a range of states, but understanding the mechanisms responsible for interspecific divergence is a challenge for evolutionary cell biology. Models are developed for the distribution of mean phenotypes likely to evolve under the joint forces of mutation and genetic drift in the face of consta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34820
更新日期:2018-06-21 00:00:00
abstract::Convenient, efficient and fast whole-brain delivery of transgenes presents a persistent experimental challenge in neuroscience. Recent advances demonstrate whole-brain gene delivery by retro-orbital injection of virus, but slow and sparse expression and the large injection volumes required make this approach cumbersom...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53639
更新日期:2020-05-18 00:00:00
abstract::The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.38578
更新日期:2018-06-29 00:00:00
abstract::Antagonistic receptive field surrounds are a near-universal property of early sensory processing. A key assumption in many models for retinal ganglion cell encoding is that receptive field surrounds are added only to the fully formed center signal. But anatomical and functional observations indicate that surrounds are...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38841
更新日期:2018-09-06 00:00:00