Abstract:
:Convenient, efficient and fast whole-brain delivery of transgenes presents a persistent experimental challenge in neuroscience. Recent advances demonstrate whole-brain gene delivery by retro-orbital injection of virus, but slow and sparse expression and the large injection volumes required make this approach cumbersome, especially for developmental studies. We developed a novel method for efficient gene delivery across the central nervous system in neonatal mice and rats starting as early as P1 and persisting into adulthood. The method employs transverse sinus injections of 2-4 μL of AAV9 at P0. Here, we describe how to use this method to label and/or genetically manipulate cells in the neonatal rat and mouse brain. The protocol is fast, simple, can be readily adopted by any laboratory, and utilizes the widely available AAV9 capsid. The procedure is adaptable for diverse experimental applications ranging from biochemistry, anatomical and functional mapping, gene expression, silencing, and editing.
journal_name
Elifejournal_title
eLifeauthors
Hamodi AS,Martinez Sabino A,Fitzgerald ND,Moschou D,Crair MCdoi
10.7554/eLife.53639subject
Has Abstractpub_date
2020-05-18 00:00:00issn
2050-084Xpii
53639journal_volume
9pub_type
杂志文章相关文献
eLife文献大全abstract::Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51461
更新日期:2020-01-17 00:00:00
abstract::The non-canonical Wnt/Ca2+ signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that the zebrafish/human phosphatidylinosit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26362
更新日期:2017-05-02 00:00:00
abstract::Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50414
更新日期:2019-10-14 00:00:00
abstract::The voltage-gated K+ channel Kv2.1 serves a major structural role in the soma and proximal dendrites of mammalian brain neurons, tethering the plasma membrane (PM) to endoplasmic reticulum (ER). Although Kv2.1 clustering at neuronal ER-PM junctions (EPJs) is tightly regulated and highly conserved, its function remains...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49953
更新日期:2019-10-30 00:00:00
abstract::Temperature-sensitive transient receptor potential (TRP) ion channels are members of the large tetrameric cation channels superfamily but are considered to be uniquely sensitive to heat, which has been presumed to be due to the existence of an unidentified temperature-sensing domain. Here we report that the homologous...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03255
更新日期:2014-07-16 00:00:00
abstract::Fruit fly neuroblasts can either self-renew, rest or take on a specialized form, depending on the levels of a protein called Prospero. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04869
更新日期:2014-10-29 00:00:00
abstract::Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27406
更新日期:2017-07-18 00:00:00
abstract::Learning to perform a complex motor task requires the optimization of specific behavioral features to cope with task constraints. We show that when mice learn a novel motor paradigm they differentially refine specific behavioral features. Animals trained to perform progressively faster sequences of lever presses to ob...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09423
更新日期:2015-09-29 00:00:00
abstract::Recently, a small-molecule communication mechanism was discovered in a range of Bacillus-infecting bacteriophages, which these temperate phages use to inform their lysis-lysogeny decision. We present a mathematical model of the ecological and evolutionary dynamics of such viral communication, and show that a communica...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58410
更新日期:2021-01-18 00:00:00
abstract::Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15798
更新日期:2016-06-21 00:00:00
abstract::Unlike passive rupture of the human chorioamnion at birth, the insect extraembryonic (EE) tissues - the amnion and serosa - actively rupture and withdraw in late embryogenesis. Withdrawal is essential for development and has been a morphogenetic puzzle. Here, we use new fluorescent transgenic lines in the beetle Tribo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13834
更新日期:2016-01-29 00:00:00
abstract::Glucocorticoid receptor (GR) is crucial for signaling mediated by stress-induced high levels of glucocorticoids. The lateral nucleus of the amygdala (LA) is a key structure underlying auditory-cued fear conditioning. Here, we demonstrate that genetic disruption of GR in the LA (LAGRKO) resulted in an auditory-cued fea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34135
更新日期:2018-06-26 00:00:00
abstract::Using knowledge of the structure of the world to infer value is at the heart of model-based reasoning and relies on a circuit that includes the orbitofrontal cortex (OFC). Some accounts link this to the representation of biological significance or value by neurons in OFC, while other models focus on the representation...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30373
更新日期:2018-03-07 00:00:00
abstract::Some DNA viruses overcome plant defenses by producing a suppressor protein that blocks the silencing of viral genes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11509
更新日期:2015-10-16 00:00:00
abstract::Using computer simulations, we generate cell-specific 3D chromosomal structures and compare them to recently published chromatin structures obtained through microscopy. We demonstrate using machine learning and polymer physics simulations that epigenetic information can be used to predict the structural ensembles of m...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60312
更新日期:2020-10-13 00:00:00
abstract::Genetic analyses of 15 species of yeast have shed new light on the divergence of gene regulation during evolution, with significant changes occurring after an event in which a whole genome was duplicated. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00933
更新日期:2013-06-18 00:00:00
abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17636
更新日期:2016-10-01 00:00:00
abstract::The gut microbiota of patients recently diagnosed with rheumatoid arthritis is enriched in microbes belonging to the Prevotella genus. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01608
更新日期:2013-11-05 00:00:00
abstract::The restoration of homeostasis after tissue damage relies on proper spatial-temporal control of damage-induced apoptosis and compensatory proliferation. In Drosophila imaginal discs these processes are coordinated by the stress response pathway JNK. We demonstrate that JNK signaling induces a dose-dependent extension ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41036
更新日期:2019-02-08 00:00:00
abstract::Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53944
更新日期:2020-01-20 00:00:00
abstract::Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis rev...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16220
更新日期:2016-07-29 00:00:00
abstract::Parvalbumin-expressing (PV+) GABAergic interneurons mediate feedforward and feedback inhibition and have a key role in gamma oscillations and information processing. The importance of fast synaptic recruitment and action potential initiation and repolarization, and rapid synchronous GABA release by PV+ cells, is well ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49872
更新日期:2019-10-28 00:00:00
abstract::AKT is implicated in neurological disorders. AKT has three isoforms, AKT1/AKT2/AKT3, with brain cell type-specific expression that may differentially influence behavior. Therefore, we examined single Akt isoform, conditional brain-specific Akt1, and double Akt1/3 mutant mice in behaviors relevant to neuropsychiatric d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56630
更新日期:2020-12-16 00:00:00
abstract::We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59980
更新日期:2020-11-09 00:00:00
abstract::Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01579
更新日期:2014-03-04 00:00:00
abstract::The speed at which a cell fate decision in nematode worms evolves is due to the number of genes that control the decision, rather than to a high mutation rate. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.62689
更新日期:2020-10-14 00:00:00
abstract::Rapid diagnostic tests (RDTs) have transformed malaria diagnosis. The most prevalent P. falciparum RDTs detect histidine-rich protein 2 (PfHRP2). However, pfhrp2 gene deletions yielding false-negative RDTs, first reported in South America in 2010, have been confirmed in Africa and Asia. We developed a mathematical mod...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25008
更新日期:2017-08-24 00:00:00
abstract::To effectively interact with their environment, humans must often select actions from multiple incompatible options. Existing theories propose that during motoric response-conflict, inappropriate motor activity is actively (and perhaps non-selectively) suppressed by an inhibitory fronto-basal ganglia mechanism. We her...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42959
更新日期:2019-05-07 00:00:00
abstract::Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity have been proposed as a substrate of memory storage. Here, we examine the impact of brief periods of sleep deprivation on dendritic structure. In mice, we find that five hours o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13424
更新日期:2016-08-23 00:00:00
abstract::Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely reflects the target cell of transformation rather than being required for continued tumor growt...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19214
更新日期:2017-01-12 00:00:00