A novel Cep120-dependent mechanism inhibits centriole maturation in quiescent cells.


:The two centrioles of the centrosome in quiescent cells are inherently asymmetric structures that differ in age, morphology and function. How these asymmetric properties are established and maintained during quiescence remains unknown. Here, we show that a daughter centriole-associated ciliopathy protein, Cep120, plays a critical inhibitory role at daughter centrioles. Depletion of Cep120 in quiescent mouse and human cells causes accumulation of pericentriolar material (PCM) components including pericentrin, Cdk5Rap2, ninein and Cep170. The elevated PCM levels result in increased microtubule-nucleation activity at the centrosome. Consequently, loss of Cep120 leads to aberrant dynein-dependent trafficking of centrosomal proteins, dispersal of centriolar satellites, and defective ciliary assembly and signaling. Our results indicate that Cep120 helps to maintain centrosome homeostasis by inhibiting untimely maturation of the daughter centriole, and defines a potentially new molecular defect underlying the pathogenesis of ciliopathies such as Jeune Asphyxiating Thoracic Dystrophy and Joubert syndrome.






Betleja E,Nanjundappa R,Cheng T,Mahjoub MR




Has Abstract


2018-05-09 00:00:00










  • Slowly folding surface extension in the prototypic avian hepatitis B virus capsid governs stability.

    abstract::Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...


    pub_type: 杂志文章


    authors: Makbul C,Nassal M,Böttcher B

    更新日期:2020-08-14 00:00:00

  • Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca²⁺ signaling.

    abstract::The final identity of a differentiated neuron is determined by multiple signaling events, including activity dependent calcium transients. Non-canonical Frizzled2 (Fz2) signaling generates calcium transients that determine neuronal polarity, neuronal migration, and synapse assembly in the developing vertebrate brain. ...


    pub_type: 杂志文章


    authors: Agrawal T,Hasan G

    更新日期:2015-05-08 00:00:00

  • Translational control of ERK signaling through miRNA/4EHP-directed silencing.

    abstract::MicroRNAs (miRNAs) exert a broad influence over gene expression by directing effector activities that impinge on translation and stability of mRNAs. We recently discovered that the cap-binding protein 4EHP is a key component of the mammalian miRNA-Induced Silencing Complex (miRISC), which mediates gene silencing. Howe...


    pub_type: 杂志文章


    authors: Jafarnejad SM,Chapat C,Matta-Camacho E,Gelbart IA,Hesketh GG,Arguello M,Garzia A,Kim SH,Attig J,Shapiro M,Morita M,Khoutorsky A,Alain T,Gkogkas CG,Stern-Ginossar N,Tuschl T,Gingras AC,Duchaine TF,Sonenberg N

    更新日期:2018-02-07 00:00:00

  • Dynamic control of hippocampal spatial coding resolution by local visual cues.

    abstract::The ability to flexibly navigate an environment relies on a hippocampal-dependent cognitive map. External space can be internally mapped at different spatial resolutions. However, whether hippocampal spatial coding resolution can rapidly adapt to local features of an environment remains unclear. To explore this possib...


    pub_type: 杂志文章


    authors: Bourboulou R,Marti G,Michon FX,El Feghaly E,Nouguier M,Robbe D,Koenig J,Epsztein J

    更新日期:2019-03-01 00:00:00

  • A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing.

    abstract::Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes...


    pub_type: 杂志文章


    authors: Liu S,Mozaffari-Jovin S,Wollenhaupt J,Santos KF,Theuser M,Dunin-Horkawicz S,Fabrizio P,Bujnicki JM,Lührmann R,Wahl MC

    更新日期:2015-07-10 00:00:00

  • High-order thalamic inputs to primary somatosensory cortex are stronger and longer lasting than cortical inputs.

    abstract::Layer (L) 2/3 pyramidal neurons in the primary somatosensory cortex (S1) are sparsely active, spontaneously and during sensory stimulation. Long-range inputs from higher areas may gate L2/3 activity. We investigated their in vivo impact by expressing channelrhodopsin in three main sources of feedback to rat S1: primar...


    pub_type: 杂志文章


    authors: Zhang W,Bruno RM

    更新日期:2019-02-11 00:00:00

  • Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma.

    abstract::Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...


    pub_type: 杂志文章


    authors: Grosso AR,Leite AP,Carvalho S,Matos MR,Martins FB,Vítor AC,Desterro JM,Carmo-Fonseca M,de Almeida SF

    更新日期:2015-11-17 00:00:00

  • Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule.

    abstract::Dyneins are motor proteins responsible for transport in the cytoplasm and the beating of axonemes in cilia and flagella. They bind and release microtubules via a compact microtubule-binding domain (MTBD) at the end of a coiled-coil stalk. We address how cytoplasmic and axonemal dynein MTBDs bind microtubules at near a...


    pub_type: 杂志文章


    authors: Lacey SE,He S,Scheres SH,Carter AP

    更新日期:2019-07-02 00:00:00

  • Exchange of water for sterol underlies sterol egress from a StARkin domain.

    abstract::Previously we identified Lam/GramD1 proteins, a family of endoplasmic reticulum membrane proteins with sterol-binding StARkin domains that are implicated in intracellular sterol homeostasis. Here, we show how these proteins exchange sterol molecules with membranes. An aperture at one end of the StARkin domain enables ...


    pub_type: 杂志文章


    authors: Khelashvili G,Chauhan N,Pandey K,Eliezer D,Menon AK

    更新日期:2019-12-04 00:00:00

  • Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

    abstract::Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...


    pub_type: 杂志文章


    authors: Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

    更新日期:2018-03-20 00:00:00

  • DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected].

    abstract::Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...


    pub_type: 杂志文章


    authors: Belin BJ,Lee T,Mullins RD

    更新日期:2015-08-19 00:00:00

  • Skin deep.

    abstract::Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...


    pub_type: 评论,杂志文章


    authors: Casas-Sánchez A,Acosta-Serrano Á

    更新日期:2016-10-14 00:00:00

  • Discovery of novel determinants of endothelial lineage using chimeric heterokaryons.

    abstract::We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq, it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern o...


    pub_type: 杂志文章


    authors: Wong WT,Matrone G,Tian X,Tomoiaga SA,Au KF,Meng S,Yamazoe S,Sieveking D,Chen K,Burns DM,Chen JK,Blau HM,Cooke JP

    更新日期:2017-03-21 00:00:00

  • BAD and KATP channels regulate neuron excitability and epileptiform activity.

    abstract::Brain metabolism can profoundly influence neuronal excitability. Mice with genetic deletion or alteration of Bad (BCL-2 agonist of cell death) exhibit altered brain-cell fuel metabolism, accompanied by resistance to acutely induced epileptic seizures; this seizure protection is mediated by ATP-sensitive potassium (KAT...


    pub_type: 杂志文章


    authors: Martínez-François JR,Fernández-Agüera MC,Nathwani N,Lahmann C,Burnham VL,Danial NN,Yellen G

    更新日期:2018-01-25 00:00:00

  • Perceptually relevant remapping of human somatotopy in 24 hours.

    abstract::Experience-dependent reorganisation of functional maps in the cerebral cortex is well described in the primary sensory cortices. However, there is relatively little evidence for such cortical reorganisation over the short-term. Using human somatosensory cortex as a model, we investigated the effects of a 24 hr gluing ...


    pub_type: 杂志文章


    authors: Kolasinski J,Makin TR,Logan JP,Jbabdi S,Clare S,Stagg CJ,Johansen-Berg H

    更新日期:2016-12-30 00:00:00

  • Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization.

    abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...


    pub_type: 杂志文章


    authors: Liu ZY,Li XF,Jiang T,Deng YQ,Ye Q,Zhao H,Yu JY,Qin CF

    更新日期:2016-10-01 00:00:00

  • Viral genome structures are optimal for capsid assembly.

    abstract::Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins and NAs with which we investigate assembly dynamics and thermodynamics. In contra...


    pub_type: 杂志文章


    authors: Perlmutter JD,Qiao C,Hagan MF

    更新日期:2013-06-14 00:00:00

  • The structure of the Ctf19c/CCAN from budding yeast.

    abstract::Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assem...


    pub_type: 杂志文章


    authors: Hinshaw SM,Harrison SC

    更新日期:2019-02-14 00:00:00

  • ILC3 GM-CSF production and mobilisation orchestrate acute intestinal inflammation.

    abstract::Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...


    pub_type: 杂志文章


    authors: Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

    更新日期:2016-01-18 00:00:00

  • Dynamic change of electrostatic field in TMEM16F permeation pathway shifts its ion selectivity.

    abstract::TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...


    pub_type: 杂志文章


    authors: Ye W,Han TW,He M,Jan YN,Jan LY

    更新日期:2019-07-18 00:00:00

  • Lipids and ions traverse the membrane by the same physical pathway in the nhTMEM16 scramblase.

    abstract::From bacteria to mammals, different phospholipid species are segregated between the inner and outer leaflets of the plasma membrane by ATP-dependent lipid transporters. Disruption of this asymmetry by ATP-independent phospholipid scrambling is important in cellular signaling, but its mechanism remains incompletely und...


    pub_type: 杂志文章


    authors: Jiang T,Yu K,Hartzell HC,Tajkhorshid E

    更新日期:2017-09-16 00:00:00

  • AP2 hemicomplexes contribute independently to synaptic vesicle endocytosis.

    abstract::The clathrin adaptor complex AP2 is thought to be an obligate heterotetramer. We identify null mutations in the α subunit of AP2 in the nematode Caenorhabditis elegans. α-adaptin mutants are viable and the remaining μ2/β hemicomplex retains some function. Conversely, in μ2 mutants, the alpha/sigma2 hemicomplex is loca...


    pub_type: 杂志文章


    authors: Gu M,Liu Q,Watanabe S,Sun L,Hollopeter G,Grant BD,Jorgensen EM

    更新日期:2013-03-05 00:00:00

  • Tracking the popularity and outcomes of all bioRxiv preprints.

    abstract::The growth of preprints in the life sciences has been reported widely and is driving policy changes for journals and funders, but little quantitative information has been published about preprint usage. Here, we report how we collected and analyzed data on all 37,648 preprints uploaded to bioRxiv.org, the largest biol...


    pub_type: 杂志文章


    authors: Abdill RJ,Blekhman R

    更新日期:2019-04-24 00:00:00

  • AMPK and vacuole-associated Atg14p orchestrate μ-lipophagy for energy production and long-term survival under glucose starvation.

    abstract::Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (...


    pub_type: 杂志文章


    authors: Seo AY,Lau PW,Feliciano D,Sengupta P,Gros MAL,Cinquin B,Larabell CA,Lippincott-Schwartz J

    更新日期:2017-04-10 00:00:00

  • Decoding gripping force based on local field potentials recorded from subthalamic nucleus in humans.

    abstract::The basal ganglia are known to be involved in the planning, execution and control of gripping force and movement vigour. Here we aim to define the nature of the basal ganglia control signal for force and to decode gripping force based on local field potential (LFP) activities recorded from the subthalamic nucleus (STN...


    pub_type: 杂志文章


    authors: Tan H,Pogosyan A,Ashkan K,Green AL,Aziz T,Foltynie T,Limousin P,Zrinzo L,Hariz M,Brown P

    更新日期:2016-11-18 00:00:00

  • Centering and symmetry breaking in confined contracting actomyosin networks.

    abstract::Centering and decentering of cellular components is essential for internal organization of cells and their ability to perform basic cellular functions such as division and motility. How cells achieve proper localization of their organelles is still not well-understood, especially in large cells such as oocytes. Here, ...


    pub_type: 杂志文章


    authors: Ierushalmi N,Malik-Garbi M,Manhart A,Abu Shah E,Goode BL,Mogilner A,Keren K

    更新日期:2020-04-21 00:00:00

  • Distinct ipRGC subpopulations mediate light's acute and circadian effects on body temperature and sleep.

    abstract::The light environment greatly impacts human alertness, mood, and cognition by both acute regulation of physiology and indirect alignment of circadian rhythms. These processes require the melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs), but the relevant downstream brain areas involved...


    pub_type: 杂志文章


    authors: Rupp AC,Ren M,Altimus CM,Fernandez DC,Richardson M,Turek F,Hattar S,Schmidt TM

    更新日期:2019-07-23 00:00:00

  • Multifactorial processes underlie parallel opsin loss in neotropical bats.

    abstract::The loss of previously adaptive traits is typically linked to relaxation in selection, yet the molecular steps leading to such repeated losses are rarely known. Molecular studies of loss have tended to focus on gene sequences alone, but overlooking other aspects of protein expression might underestimate phenotypic div...


    pub_type: 杂志文章


    authors: Sadier A,Davies KT,Yohe LR,Yun K,Donat P,Hedrick BP,Dumont ER,Dávalos LM,Rossiter SJ,Sears KE

    更新日期:2018-12-18 00:00:00

  • BRAF activates PAX3 to control muscle precursor cell migration during forelimb muscle development.

    abstract::Migration of skeletal muscle precursor cells is a key step during limb muscle development and depends on the activity of PAX3 and MET. Here, we demonstrate that BRAF serves a crucial function in formation of limb skeletal muscles during mouse embryogenesis downstream of MET and acts as a potent inducer of myoblast cel...


    pub_type: 杂志文章


    authors: Shin J,Watanabe S,Hoelper S,Krüger M,Kostin S,Pöling J,Kubin T,Braun T

    更新日期:2016-12-01 00:00:00

  • Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis.

    abstract::Oligomeric assemblies of intraflagellar transport (IFT) particles build cilia through sequential recruitment and transport of ciliary cargo proteins within cilia. Here we present the 1.8 Å resolution crystal structure of the Chlamydomonas IFT-B protein IFT80, which reveals the architecture of two N-terminal β-propelle...


    pub_type: 杂志文章


    authors: Taschner M,Lorentzen A,Mourão A,Collins T,Freke GM,Moulding D,Basquin J,Jenkins D,Lorentzen E

    更新日期:2018-04-16 00:00:00