Abstract：
:KCNQ1 encodes a potassium voltage-gated channel and represents a susceptibility locus for type 2 diabetes mellitus (T2DM). Here, we explored the association between KCNQ1 polymorphisms and hypertension risk in individuals with T2DM, as well as the role of KCNQ1 in vascular smooth muscle cell contraction in vitro. To investigate the relationship between KCNQ1 and the risk of developing hypertension in patients with T2DM, we divided the T2DM cohort into hypertension (n = 452) and non-hypertension (n = 541) groups. The Mann-Whitney U test, chi-square test, and multivariate regression analyses were used to assess the clinical characteristics and genotypic frequencies. In vitro studies utilized the rat aortic smooth muscle A10 cell line. Patients in the hypertension group were significantly older at the time of enrollment and had higher levels of body mass index, waist-to-hip ratio, and triglyceride than those in the non-hypertension group. The KCNQ1 rs3864884 and rs12576239 genetic variants were associated with hypertension in T2DM. KCNQ1 expression was lower in the individuals with the CC versus the CT and TT genotypes. Smooth muscle cell contractility was inhibited by treatment with a KCNQ1 inhibitor. These results suggest that KCNQ1 might be associated with hypertension in individuals with T2DM.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Huang KC,Li TM,Liu X,Chen JH,Chien WK,Shiao YT,Tsang H,Lin TH,Liao CC,Huang SM,Li JP,Lin CW,Lin JC,Lin CC,Lai CH,Cheng CF,Liang WM,Hung CH,Chen CC,Lin YJ,Tsai FJdoi
10.1002/jcp.25775subject
Has Abstractpub_date
2017-12-01 00:00:00pages
3309-3316issue
12eissn
0021-9541issn
1097-4652journal_volume
232pub_type
杂志文章abstract：:Lung cancer (LC) is the leading cause of cancer-related deaths all over the world, among both men and women, with an incidence of over 200,000 new cases per year coupled with a very high mortality rate. LC comprises of two major clinicopathological categories: small-cell (SCLC) and nonsmall-cell lung carcinoma (NSCLC)...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.26607
更新日期：2018-04-20 00:00:00
abstract：:Cells from kidney proximal tubules have been successfully isolated, characterized, and cultured from male Fischer 344 rats between 150-400 g using a two-step collagenase perfusion. The cells undergo high levels of DNA synthesis and mitosis in both serum free media (with an without hormone supplementation) and media co...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041310116
更新日期：1987-04-01 00:00:00
abstract：:The specific activity of a peroxisomal enzyme, lactate oxidase, and of pyruvate kinase and lactate dehydrogenase, which are not peroxisomal, increased rapidly when shaken cultures of Tetrahymena were transferred to conditions of oxygen restriction and supplemented with glucose. Two other peroxisomal enzymes, catalase ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040850106
更新日期：1975-02-01 00:00:00
abstract：:Long noncoding RNA (lncRNA) NKILA has been well studied in several types of human tumors as a tumor suppressor, while its involvement in cervical squamous cell carcinoma (CSCC) remains unclear. In our studies, we found that serum NKILA was at lower levels and serum microRNA-21 (miRNA-21) was at higher levels in patien...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29439
更新日期：2020-11-01 00:00:00
abstract：:Resistin, secreted by macrophages in tumor microenvironment, has never been investigated in pancreatic cancer models, despite a vibrant tumor microenvironment around pancreatic tumors. We evaluated serum resistin levels in healthy individuals versus pancreatic cancer patients representing different tumor grades. In vi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27631
更新日期：2019-06-01 00:00:00
abstract：:Proteinuria in diabetic nephropathy has been correlated with reduction in heparan sulfate proteoglycan (HSPG) content of the glomerular basement membrane. We have previously shown that the underlying mechanism probably involves reduction in the synthesis by glomerular epithelial cells. In this study we explored whethe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199604)167:1<131::AID-JCP1
更新日期：1996-04-01 00:00:00
abstract：:Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degradi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390107
更新日期：1989-04-01 00:00:00
abstract：:Exposure to D-allose has been demonstrated to lead to decreased 2-deoxy-D-glucose (2-DG) and 3-0-methyl-D-glucose transport in the V79 Chinese hamster lung fibroblast cell line. The effect of D-allose 1) was maximal after 4 hours exposure to the cells; 2) was optimal between 2.77 and 5.55 mM D-allose; and 3) led to a ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041450217
更新日期：1990-11-01 00:00:00
abstract：:The development of atherosclerotic plaque is associated with neovascularization in the thickened intima and media of vascular walls. Neovascularization may have a role in the progression of atherosclerotic plaque as well as in the development of intraplaque hemorrhage. However, the mechanism and stimulus for neovascul...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041640324
更新日期：1995-09-01 00:00:00
abstract：:Colorectal cancer (CRC) is the third most common cancer and a leading cause of cancer-related death worldwide. About 1.8 million new cases were reported in 2018. Accordingly, there is an urgent need for development of effective strategies to lower the mortality and morbidity originated from this malignancy. MicroRNAs ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28872
更新日期：2019-12-01 00:00:00
abstract：:Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20927
更新日期：2007-04-01 00:00:00
abstract：:BHK fibroblasts can be growth arrested by incubation in low serum (0.1%) medium. Growth is initiated by incubating cells in high serum (10%) medium. We have found that if the quiescent cells in low serum medium are incubated with insulin, the G0 to S transit time is decreased by two to six hours when serum (10%) is ad...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041050318
更新日期：1980-12-01 00:00:00
abstract：:Sevoflurane has been commonly utilized in nonobstetric surgeries in pregnant women, and its impacts on fetal brain are still not completely known. Ectopic NR4A2 expression has been reported to be related with familial Parkinson disease, and through dual luciferase we found that NR4A2 is a target gene of microRNA-183 (...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27158
更新日期：2019-04-01 00:00:00
abstract：:The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Mul...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199802)174:2<145::AID-JCP1
更新日期：1998-02-01 00:00:00
abstract：:Clinicians have observed that keloids preferentially form in body areas subject to increased skin tension. We hypothesized a difference exists in the transcriptional response of keloid fibroblasts to mechanical strain compared with normal fibroblasts. Normal and keloid fibroblasts were seeded in a device calibrated to...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20486
更新日期：2006-02-01 00:00:00
abstract：:Protein-DNA interactions within the promoter of a cell cycle-regulated human H4 histone gene were examined by binding of 5'-end-labeled DNA segments to Western blots of nuclear protein fractions. Specific protein interactions were observed with DNA segments located between -500 bp and -1,070 bp upstream of the ATG ini...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390214
更新日期：1989-05-01 00:00:00
abstract：:The biological activities of crocin, one of the main bioactive compounds of saffron, include anti-inflammatory, antioxidant, antidepressant, and anticancer effects. Crocin has been shown to trigger the apoptosis of gastric cancer cells, but its effect on the metastasis of gastric cancer cells remains unclear. Krüppel-...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28418
更新日期：2019-08-01 00:00:00
abstract：:Characteristic features like self-renewal, multilineage differentiation potential, and immune-modulatory/anti-inflammatory properties, besides the ability to mobilize and home distant tissues make stem cells (SCs) a lifeline for an individual. Stem cells (SCs) if could be harvested and expanded without any abnormal ch...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.27846
更新日期：2019-06-01 00:00:00
abstract：:S100B, a Ca(2+)-modulated protein with both intracellular and extracellular regulatory roles, is most abundant in astrocytes, is expressed in various amounts in several non-nervous cells and is also found in normal serum. Astrocytes secrete S100B, and extracellular S100B exerts trophic and toxic effects on neurons dep...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10462
更新日期：2004-05-01 00:00:00
abstract：:Sodium butyrate (6 mM) blocks the resumption of the cell division cycle in serum-deprived chemically transformed Balb/c-3T3 mouse fibroblasts (BP-A31). The inhibition of G1 progression by sodium butyrate is not restricted to a specific mitogenic signaling pathway and is equally effective when tetradecanoyl phorbol ace...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041450108
更新日期：1990-10-01 00:00:00
abstract：:An efficient regulation of fuel metabolism in response to internal and environmental stimuli is a vital task that requires an intact carnitine system. The carnitine system, comprehensive of carnitine, its derivatives, and proteins involved in its transformation and transport, is indispensable for glucose and lipid met...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1097-4652(200003)182:3<339::AID-JCP4
更新日期：2000-03-01 00:00:00
abstract：:Cytoskeleton not only controls cell morphology but also regulates cell growth, migration, differentiation, and gene expression, events which are fundamental to embryogenesis, carcinogenesis, and wound healing. We have recently reported that reorganization of cytoskeleton induces expression of mRNA for transforming gro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199708)172:2<192::AID-JCP6
更新日期：1997-08-01 00:00:00
abstract：:Mouse heptoma cells that secrete large amounts of alpha-fetoprotein (AFP) and albumin have been crossed with rat hepatoma cells that secret only albumin, and in relatively small amounts, to investigate the influence of each parental genome upon the expression of serum proteins. All of the ten independent hybrid clones...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041040304
更新日期：1980-09-01 00:00:00
abstract：:The effect of retinoic acid on human fibroblasts was studied in a cell culture model of chronologic aging and photoaging. During early exponential phase, all trans-retinoic acid significantly stimulated growth rate of adult arm-derived dermal fibroblasts but not of newborn or adult foreskin-derived fibroblasts. Retino...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390117
更新日期：1989-04-01 00:00:00
abstract：:Conditioned medium from Sertoli cells, prepared from testes of 20-day-old rats, contains component(s) that inhibit the incorporation of [3H]-thymidine into DNA of peritubular myoid cells (PMC) and inhibit the proliferation of PMC. These components are trypsin-resistant, heat-stable compounds having a molecular weight ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041470313
更新日期：1991-06-01 00:00:00
abstract：:A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., '71) is described. The use of mechanically dissociated single cells labeled with 3H-leucine, and of a gyratory shaker increased considerably the efficiency of cell collection. With chick embryo neur...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900104
更新日期：1977-01-01 00:00:00
abstract：:The human cervical cancer (CC) has been identified as one of the most common tumors in women, and the molecular regulation in CC still remains unclear. The dysregulation of periostin has been found in a variety of cancers, but whether it is involved in the regulation of CC is unknown. The present study aimed to invest...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28113
更新日期：2019-08-01 00:00:00
abstract：:The development of efficient and repeatable protocols for biobanking and prolonged storage of cancer stem cells (CSCs), with minimum alterations in biological function, is valuable and desired, particularly for retrospective analysis and clinical applications. In particular, data regarding the effect of cryopreservatio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29150
更新日期：2020-03-01 00:00:00
abstract：:Abnormal glucose handling in the proximal tubule may play an important role in the development of diabetic nephropathy. Thus, the present study was designed to examine the effect of high glucose on alpha-methyl-D-glucopyranoside (alpha-MG) uptake and its signaling pathways in the primary cultured rabbit renal proximal...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(200006)183:3<355::AID-JCP8
更新日期：2000-06-01 00:00:00
abstract：:Hydrocortisone and dexamethasone produced a time-dependent increase [125I]epidermal growth factor [( 125I]EGF) binding in primary cultures of isolated rat hepatocytes. Maximally effective doses of glucocorticoids resulted in a 70-100% increase in binding. The effect was similar when hepatocytes were maintained on coll...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041190304
更新日期：1984-06-01 00:00:00
