Butyrate blocks the accumulation of CDC2 mRNA in late G1 phase but inhibits both the early and late G1 progression in chemically transformed mouse fibroblasts BP-A31.

Abstract:

:Sodium butyrate (6 mM) blocks the resumption of the cell division cycle in serum-deprived chemically transformed Balb/c-3T3 mouse fibroblasts (BP-A31). The inhibition of G1 progression by sodium butyrate is not restricted to a specific mitogenic signaling pathway and is equally effective when tetradecanoyl phorbol acetate (TPA), insulin, or fetal calf serum (FCS) is used as inducer. The inhibitor acts in early as well as late G1 phase as indicated by experiments in which inhibitor was added and withdrawn at different times after restimulation of quiescent cells by FCS. At the gene expression level, sodium butyrate does not affect the inducibility of early cell cycle-related genes (c-myc, c-jun) while blocking the induction of cdc 2 mRNA, a late G1 marker. We conclude that sodium butyrate does not interfere with the growth factor signaling pathways regulating the (early) cell cycle-related gene expression. However, the presence of sodium butyrate early in G1 phase inhibits the cascade of events leading eventually to the expression of late G1-characteristic genes such as cdc2. The antimitogenic activity of sodium butyrate may be related to its interference with an (unknown) process involved in the "mitogenic" cascade.

journal_name

J Cell Physiol

authors

Charollais RH,Buquet C,Mester J

doi

10.1002/jcp.1041450108

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

46-52

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

145

pub_type

杂志文章
  • Upregulation of microRNA-218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1.

    abstract::This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum of patients with CAD ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29214

    authors: Gao W,Cui H,Li Q,Zhong H,Yu J,Li P,He X

    更新日期:2020-03-01 00:00:00

  • Cytoplasmic juxtamembrane domain of the human EGF receptor is required for basolateral localization in MDCK cells.

    abstract::Although it is well established that epidermal growth factor receptors (EGFRs) are asymmetrically expressed at the basolateral plasma membrane in polarized epithelial cells, how this process is regulated is not known. The purpose of this study was to address the mechanism of directed EGFR basolateral sorting using the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041620316

    authors: Hobert M,Carlin C

    更新日期:1995-03-01 00:00:00

  • Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.

    abstract::Cell cultures have seen much progress in the numbers available cell lines, their applications and culture techniques. Three dimensional cultures and co-cultures are examples of strategies that bring in vitro conditions closer to natural in vivo systems. We describe here, the formation of cell aggregates in three-dimen...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24433

    authors: Ravi M,Sah S,Bhammar R

    更新日期:2014-02-01 00:00:00

  • Growth kinetics as a function of ploidy in diploid, tetraploid, and octaploid smooth muscle cells derived from the normal rat aorta.

    abstract::The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250322

    authors: Rosen EM,Goldberg ID,Shapiro HM,Zoller LC,Myrick KV,Levenson SE,Halpin PA

    更新日期:1985-12-01 00:00:00

  • Differential regulation of the lateral mobility of plasma membrane phospholipids by the extracellular matrix and cholesterol.

    abstract::In this study, we compared qualitative and quantitative changes in the lateral mobility of phospholipid molecules in the plasma membrane of intact cells under various conditions of specific interaction of integrins in the cell membrane with two extracellular matrix (ECM) components viz. fibronectin (FN) and laminin (L...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21339

    authors: Ramprasad OG,Rangaraj N,Srinivas G,Thiery JP,Dufour S,Pande G

    更新日期:2008-05-01 00:00:00

  • Transforming activity of mutant human p53 alleles.

    abstract::Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041480309

    authors: Slingerland JM,Benchimol S

    更新日期:1991-09-01 00:00:00

  • Thymic epithelial cells synthesize a heparan sulfate with a highly sulfated region.

    abstract::Epithelial cells are important components of the thymus microenvironment and are involved in thymocyte differentiation. The production and secretion of sulfated glycosaminoglycans by these cells grown in culture were investigated using labeling with radioactive 35S-Na2SO4 and 3H-glucosamine. The major glycosaminoglyca...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199901)178:1<51::AID-JCP7>

    authors: Werneck CC,Oliveira-Dos-Santos AJ,Silva LC,Villa-Verde DM,Savino W,Mourão PA

    更新日期:1999-01-01 00:00:00

  • Parathyroid hormone-related protein (107-139) increases human osteoblastic cell survival by activation of vascular endothelial growth factor receptor-2.

    abstract::Parathyroid hormone-related protein (PTHrP) (107-139), in contrast to the N-terminal fragment PTHrP (1-36), has been shown to interact with the vascular endothelial growth factor (VEGF) system to modulate human osteoblast differentiation. In this study, we evaluated whether this interaction might affect human osteobla...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21547

    authors: Alonso V,de Gortázar AR,Ardura JA,Andrade-Zapata I,Alvarez-Arroyo MV,Esbrit P

    更新日期:2008-12-01 00:00:00

  • Identification of novel, stage-specific polypeptides associated with the differentiation of mammary epithelial stem cells to alveolar-like cells in culture.

    abstract::A linear pathway of morphologically intermediate cells has been identified between the cuboidal epithelial stem cells and the doming alveolar-like cultures of the cell line Rat Mammary (Rama) 25 in the order: cuboidal----grey----dark----dark droplet cell----doming cultures. The overall process can be accelerated by di...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240324

    authors: Paterson FC,Rudland PS

    更新日期:1985-09-01 00:00:00

  • p53-Mediated enhancement of radiosensitivity by selenophosphate synthetase 1 overexpression.

    abstract::Selenium has been associated with cancer prevention. Despite vast knowledge of selenium effect on various health conditions, functional characterization of selenium metabolic enzymes on cellular physiology has been limited. Therefore, to gain insight into the mechanisms underlying cancer prevention by selenium, we inv...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20714

    authors: Chung HJ,Yoon SI,Shin SH,Koh YA,Lee SJ,Lee YS,Bae S

    更新日期:2006-10-01 00:00:00

  • Suppression of platelet-derived growth factor alpha- and beta-receptor mRNA levels in human fibroblasts by SV40 T/t antigen.

    abstract::It is known that down-regulation of cell surface platelet-derived growth factor (PDGF) receptors accompanies transformation by SV40. In this work human embryonic lung fibroblasts were used as a model system to study the effects of SV 40 on PDGF receptor expression. It is shown that transformation by SV 40 early region...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199601)166:1<12::AID-JCP2>

    authors: Wang JL,Nistér M,Bongcam-Rudloff E,Pontén J,Westermark B

    更新日期:1996-01-01 00:00:00

  • Glucocorticoids and prostaglandins inhibit the induction of macrophage DNA synthesis by macrophage growth factor and phorbol ester.

    abstract::The tumor-promoting phorbol ester, 12-0-tetradecanoyl-phorbol-13-acetate (TPA), stimulates starch-elicited mouse peritoneal macrophages to undergo DNA synthesis in vitro, apparently without the generation of an endogenous macrophage growth factor (MGF). No evidence was found for any synergistic interaction between TPA...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041150111

    authors: Hamilton JA

    更新日期:1983-04-01 00:00:00

  • Ursolic acid ameliorates CCl4-induced liver fibrosis through the NOXs/ROS pathway.

    abstract::Liver fibrosis is a reversible wound-healing response that occurs after liver injury. NADPH oxidases (NOXs) and reactive oxygen species (ROS) which are expressed in hepatocytes (HCs), hepatic stellate cells (HSCs), and Kupffer cells (KCs) play an important role in the development of hepatic fibrosis. In in vitro studi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26541

    authors: Gan D,Zhang W,Huang C,Chen J,He W,Wang A,Li B,Zhu X

    更新日期:2018-10-01 00:00:00

  • Glutathione stimulates A549 cell proliferation in glutamine-deficient culture: the effect of glutamate supplementation.

    abstract::Extracellular glutathione (GSH) is degraded by an external cell-surface enzyme, gamma-glutamyltranspeptidase (gamma-GT). The products are transported into cells to participate in important cellular processes. In the present study, we tested the hypothesis that extracellular GSH is a source of glutamic acid for cells t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041610323

    authors: Kang YJ,Feng Y,Hatcher EL

    更新日期:1994-12-01 00:00:00

  • Oxidative stress induces GLUT4 translocation by activation of PI3-K/Akt and dual AMPK kinase in cardiac myocytes.

    abstract::In response to metabolic stress, GLUT4, the most abundant glucose transporter, translocates from intracellular vesicles to the plasma membrane. This appears to play an important role in protecting cardiac myocytes from ischemic injury. To investigate the precise mechanisms of GLUT4 translocation in cardiomyocytes, we ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21353

    authors: Horie T,Ono K,Nagao K,Nishi H,Kinoshita M,Kawamura T,Wada H,Shimatsu A,Kita T,Hasegawa K

    更新日期:2008-06-01 00:00:00

  • A PPARs cross-talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis.

    abstract::Peroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPARbeta and PPARgamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPARbeta is known to ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21509

    authors: Leisewitz AV,Urrutia CR,Martinez GR,Loyola G,Bronfman M

    更新日期:2008-11-01 00:00:00

  • Nuclear lamina assembly in the first cell cycle of rat liver regeneration.

    abstract::The nuclear lamina is a fibrous structure at the nucleoplasmic surface of the inner nuclear membrane. Its assembly state is regulated by phosphorylation of its protein components, the lamins A, B, and C. The isoprenylation of the lamins is essential for their proper membrane anchoring and functionality. The content an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199705)171:2<135::AID-JCP3

    authors: Bruscalupi G,Di Croce L,Lamartina S,Zaccaria ML,Luzzatto AC,Trentalance A

    更新日期:1997-05-01 00:00:00

  • Binding of Salmonella minnesota R-form glycolipid mR595 to rat fibroblasts and its effect on cell metabolism and cell behaviour.

    abstract::Trypsinized normal rat embryo fibroblasts and untrypsinized and trypsinized transformed rat fibroblasts have two orders of binding sites for bacterial glycolipid mR595. The high order sites fix 1--3 micrograms glycolipid mR595/10(5) cells and those of the low order fix about 6 microgram glycolipids mR595/10(6) cells. ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040980113

    authors: Lallier R,Trudel M,Brailovsky CA,Nigam VN

    更新日期:1979-01-01 00:00:00

  • Regulated localization confers multiple functions on the protease urokinase plasminogen activator.

    abstract::We have investigated the role of the plasminogen activation cascade in skeletal muscle differentiation. Migrating, undifferentiated myoblasts express urokinase plasminogen activator (uPA) and its cell surface receptor (uPAR). Consequently, uPA is localized predominantly to the cell surface. Preventing uPA from associa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199705)171:2<217::AID-JCP1

    authors: Wells JM,Strickland S

    更新日期:1997-05-01 00:00:00

  • Granulocyte-macrophage colony formation in serum-free culture: effects of purified colony-stimulating factors and modulation by hydrocortisone.

    abstract::The effects of three purified colony-stimulating factors (CSFs) with different specificities for the granulocyte (G) and macrophage (M) lineages (G-CSF, CSF-1 and GM-CSF) were studied in a serum-free clonal assay system. The results were compared with those obtained in similar cultures containing fetal calf serum (FCS...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041280214

    authors: Eliason JF

    更新日期:1986-08-01 00:00:00

  • The role of caldesmon in the regulation of endothelial cytoskeleton and migration.

    abstract::The actin- and myosin-binding protein, caldesmon (CaD) is an essential component of the cytoskeleton in smooth muscle and non-muscle cells and is involved in the regulation of cell contractility, division, and assembly of actin filaments. CaD is abundantly present in endothelial cells (EC); however, the contribution o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20244

    authors: Mirzapoiazova T,Kolosova IA,Romer L,Garcia JG,Verin AD

    更新日期:2005-06-01 00:00:00

  • Structure of transcriptionally active chromatin: radiological evidence for requirement of torsionally constrained DNA.

    abstract::Synthesis of alpha- and beta-globin RNA in DMSO-induced Friend's erythroleukemia cells and synthesis of immunoglobulin gamma- and kappa-chain RNA, total RNA, 5S RNA, and tRNA in mouse myeloma cells (MPC-11) was inhibited by gamma-irradiation. For all RNA species, synthesis decreased nearly exponentially as a function ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041410216

    authors: Rodi CP,Sauerbier W

    更新日期:1989-11-01 00:00:00

  • Effects of interleukin-1 on fibroblast extracellular matrix, using a 3-dimensional culture system.

    abstract::This study describes the alterations induced by Interleukin-1 alpha and -beta (IL-1 alpha and IL-1 beta) on fibroblast-synthesized extracellular matrix. Fibroblasts were grown between pieces of dentin or in collagen-coated Terasaki wells for 3 or 6-9 weeks to create 3-dimensional cell-containing matrices constituted p...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041390308

    authors: Qwarnström EE,MacFarlane SA,Page RC

    更新日期:1989-06-01 00:00:00

  • The circ_0021977/miR-10b-5p/P21 and P53 regulatory axis suppresses proliferation, migration, and invasion in colorectal cancer.

    abstract::An in-depth understanding of circular RNAs (circRNAs) indicates that they are abundant in the eukaryotic transcriptome. Many circRNAs act as microRNA sponges; thus, they represent a new type of regulatory factor. However, the role of circRNA in colorectal cancer (CRC) remains largely unknown. Low circ_0021977 expressi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29135

    authors: Lu C,Jiang W,Hui B,Rong D,Fu K,Dong C,Tang W,Cao H

    更新日期:2020-03-01 00:00:00

  • Potassium-induced reverse transformation of cells infected with a temperature-sensitive transformation mutant virus.

    abstract::High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250213

    authors: Lai CN,Becker FF

    更新日期:1985-11-01 00:00:00

  • Constitutive secretion of beta-trace protein by cultivated porcine choroid plexus epithelial cells: elucidation of its complete amino acid and cDNA sequences.

    abstract::Primary porcine choroid plexus epithelial cells cultivated in chemically defined medium maintain their epithelial characteristics and form confluent monolayers. They produce a fluid the composition of which resembles cerebrospinal fluid. The present study demonstrates constitutive secretion of large amounts of beta-tr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199611)169:2<235::AID-JCP2

    authors: Hoffmann A,Gath U,Gross G,Lauber J,Getzlaff R,Hellwig S,Galla HJ,Conradt HS

    更新日期:1996-11-01 00:00:00

  • Antibodies Against Mimotopes of Simian Virus 40 Large T Antigen, the Oncoprotein, in Serum Samples From Elderly Healthy Subjects.

    abstract::Simian Virus 40 (SV40), a monkey polyomavirus, was administered to human populations by early anti-poliomylitis vaccines contaminated by this small DNA tumor virus. Data on SV40 infection in humans remain controversial. Elderly subjects represent an interesting cohort to investigate, because they were not immunized wi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25405

    authors: Mazzoni E,Guerra G,Casali MV,Pietrobon S,Bononi I,Puozzo A,Tagliapietra A,Nocini PF,Tognon M,Martini F

    更新日期:2017-01-01 00:00:00

  • The effect of medium pH on rate of growth, neurite formation and acetylcholinesterase activity in mouse neuroblastoma cells in culture.

    abstract::Cell division, neurite formation and acetylcholinesterase activity were examined in a clone (NBA2) of mouse neuroblastoma cells maintained for up to 120 hours in medium with pH values between 6.6 and 8.0. Growth rate decreased as pH was reduced from 7.8 to 6.6. Generation time at pH 7.4 was 25 hours, while the rate of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040910107

    authors: Bear MP,Schneider FH

    更新日期:1977-04-01 00:00:00

  • Biological effects of insulin and its analogs on cancer cells with different insulin family receptor expression.

    abstract::Hyperinsulinemia is a likely cause of the increased cancer incidence and mortality in diabetic patients, but its role is difficult to define in vivo. Previous in vitro studies testing the mitogenic potential of insulin and its analogs provided incomplete and sometimes contradictory results. To better evaluate cancer c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24635

    authors: Sciacca L,Cassarino MF,Genua M,Vigneri P,Giovanna Pennisi M,Malandrino P,Squatrito S,Pezzino V,Vigneri R

    更新日期:2014-11-01 00:00:00

  • Interaction of rat asialotransferrin with adult rat hepatocytes: its relevance for iron uptake and protein degradation.

    abstract::Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041350325

    authors: Rudolph JR,Regoeczi E

    更新日期:1988-06-01 00:00:00