Abstract：
:Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen-chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentiation, and regeneration. Rabbit chondrocytes were labeled with magnetic nanoparticles and characterized by scanning electron microscopy (SEM), transmission electron (TEM) microscopy, and gene and protein expression analyses. The experimental results showed that the magnetic nanoparticles did not affect the phenotype of chondrocytes after cell labeling, nor were protein and gene expression affected. The biphasic type II collagen-chitosan/poly(lactic-co-glycolic) acid scaffold was characterized by SEM, and labeled chondrocytes showed a homogeneous distribution throughout the scaffold after cultivation onto the polymer. Cellular phenotype remained unaltered but with increased gene expression of type II collagen and aggrecan, as indicated by cell staining, indicating chondrogenesis. Decreased SRY-related high mobility group-box gene (Sox-9) levels of cultured chondrocytes indicated that differentiation was associated with osteogenesis. These results are encouraging for the development of techniques for trackable cartilage regeneration and osteochondral defect repair which may be applied in vivo and, eventually, in clinical trials.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Su JY,Chen SH,Chen YP,Chen WCdoi
10.3390/ijms18010087subject
Has Abstractpub_date
2017-01-04 00:00:00issue
1issn
1422-0067pii
ijms18010087journal_volume
18pub_type
杂志文章abstract：:Claudins regulate paracellular permeability in different tissues. The claudin-binding domain of Clostridium perfringens enterotoxin (cCPE) is a known modulator of a claudin subset. However, it does not efficiently bind to claudin-1 (Cldn1). Cldn1 is a pharmacological target since it is (i) an essential co-receptor for...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194774
更新日期：2019-09-26 00:00:00
abstract：:Lattice degeneration involves thinning of the retina that occurs over time. Here we performed an immunohistological study of tissue sections of human peripheral retinal lattice degeneration to investigate if retinal pigment epithelium (RPE) cells are involved in the pathogenesis of this condition. In two cases of reti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197347
更新日期：2020-10-05 00:00:00
abstract：:Increasing scientific evidence demonstrated the deregulation of human endogenous retroviruses (HERVs) expression in complex diseases, such as cancer, autoimmune, psychiatric, and neurological disorders. The dynamic regulation of HERV activity and their responsiveness to a variety of environmental stimuli designate HER...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113286
更新日期：2018-10-23 00:00:00
abstract：:Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we inves...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051020
更新日期：2017-05-09 00:00:00
abstract：:The mechanisms behind fatigue intensification during cancer therapy remain elusive. The interferon alpha-inducible protein 27 (IFI27) was the most up-regulated gene based on our previous microarray data in fatigued men with non-metastatic prostate cancer receiving localized external beam radiation therapy (EBRT). The ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140816943
更新日期：2013-08-16 00:00:00
abstract：:Stimulation of thermogenesis in brown adipose tissue (BAT) could have far-reaching health benefits in combatting obesity and obesity-related complications. Apolipoprotein A-IV (ApoA-IV), produced by the gut and the brain in the presence of dietary lipids, is a well-known short-term satiating protein. While our previou...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2021-01-27 00:00:00
abstract：:Viral hemorrhagic septicemia virus genotype IVb (VHSV-IVb) is presently found throughout the Laurentian Great Lakes region of North America. We recently developed a DNA vaccine preparation containing the VHSV-IVb glycoprotein (G) gene with a cytomegalovirus (CMV) promoter that proved highly efficacious in protecting m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17111898
更新日期：2016-11-15 00:00:00
abstract：:RNA virus genomes are multifunctional entities endowed with conserved structural elements that control translation, replication and encapsidation, among other processes. The preservation of these structural RNA elements constraints the genomic sequence variability. The hepatitis C virus (HCV) genome is a positive, sin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041479
更新日期：2020-02-21 00:00:00
abstract：:The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15034583
更新日期：2014-03-17 00:00:00
abstract：:Congenital cataracts are the prime cause for irreversible blindness in children. The global incidence of congenital cataract is 2.2-13.6 per 10,000 births, with the highest prevalence in Asia. Nearly half of the congenital cataracts are of familial nature, with a predominant autosomal dominant pattern of inheritance. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249569
更新日期：2020-12-16 00:00:00
abstract：:In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed F...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140919146
更新日期：2013-09-17 00:00:00
abstract：:Citalopram is a selective serotonin reuptake inhibitor, and although widely used as an antidepressant, this drug has also demonstrated interesting repairing properties leading to motor recovery and pathology amelioration in animal models of stroke and degeneration. Here, we tested the efficacy of both 7-day and 8-week...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21145062
更新日期：2020-07-17 00:00:00
abstract：:Paracrine factors can induce cardiac regeneration and repair post myocardial infarction by stimulating proliferation of cardiac cells and inducing the anti-fibrotic, antiapoptotic, and immunomodulatory effects of angiogenesis. Here, we screened a human secretome library, consisting of 923 growth factors, cytokines, an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20236037
更新日期：2019-11-30 00:00:00
abstract：:The concept of "Sweet Immunity" postulates that sugar metabolism and signaling influence plant immune networks. In this study, we tested the potential of commercially available inulin-type fructans to limit disease symptoms caused by Botrytis cinerea in lettuce. Spraying mature lettuce leaves, with inulin-type fructan...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051052
更新日期：2019-02-28 00:00:00
abstract：:Nicotine addiction is a severe public health problem. The aim of this study was to investigate the alterations in key neurotransmissions after 60 days of withdrawal from seven weeks of intermittent cigarette smoke, e-cigarette vapours, or an e-cigarette vehicle. In the nicotine withdrawal groups, increased depressive ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020599
更新日期：2021-01-09 00:00:00
abstract：:We characterized Schistosoma japonicum HSP40 (Sjp40) and HSP90α (Sjp90α) in this study. Western blot analysis revealed both are present in soluble egg antigens and egg secretory proteins, implicating them in triggering the host immune response after secretion from eggs into host tissues. These observations were confir...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114034
更新日期：2020-06-04 00:00:00
abstract：:The maturation of iPSC-derived cardiomyocytes is still a critical point for their application in cardiovascular research as well as for their clinical use. Although multiple differentiation protocols have been established, researchers failed to generate fully mature cardiomyocytes in vitro possessing identical phenoty...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082819
更新日期：2020-04-17 00:00:00
abstract：:Proteins regulate many cellular functions and analyzing the presence and abundance of proteins in biological samples are central focuses in proteomics. The discovery and validation of biomarkers, pathways, and drug targets for various diseases can be accomplished using mass spectrometry-based proteomics. However, with...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms16023537
更新日期：2015-02-05 00:00:00
abstract：:Targeting cancer cells using chimeric-antigen-receptor (CAR-)T cells has propelled adoptive T-cell therapy (ATT) to the next level. A plentitude of durable complete responses using CD19-specific CAR-T cells in patients suffering from various lymphoid malignancies resulted in the approval by the food and drug administr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20235942
更新日期：2019-11-26 00:00:00
abstract：:The objectives of this study were to evaluate the binding behavior of Cu(II) and Eu(III) with water extractable organic matter (WEOM) in soil, and assess the competitive effect of the cations. Excitation-emission matrix (EEM) fluorescence spectrometry was used in combination with parallel factor analysis (PARAFAC) to ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160714464
更新日期：2015-06-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18081743
更新日期：2017-08-11 00:00:00
abstract：:Magnetic biomimetic scaffolds of poly(L-lactide) (PLLA) and nanoparticles of magnetite (nFe3O4) are prepared in a wide ratio of compositions by lyophilization for bone regeneration. The magnetic properties, cytotoxicity, and the in vitro degradation of these porous materials are closely studied. The addition of magnet...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194664
更新日期：2019-09-20 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197332
更新日期：2020-10-04 00:00:00
abstract：:Bioelectrochemical systems (BES) are groups of bioelectrochemical technologies and platforms that could facilitate versatile environmental and biological applications. The performance of BES is mainly determined by the key process of electron transfer at the bacteria and electrode interface, which is known as extracel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18010090
更新日期：2017-01-04 00:00:00
abstract：:A spontaneous mutation in the lysosomal acid phosphatase (Acp2) enzyme (nax: naked-ataxia) in experimental mice results in delayed hair appearance and severe cytoarchitectural impairments of the cerebellum, such as a Purkinje cell (PC) migration defect. In our previous investigation, our team showed that Acp2 expressi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082914
更新日期：2020-04-21 00:00:00
abstract：:The aim of the present study was to determine aflatoxin levels in peanut products traded in the Northeast region of São Paulo, Brazil. To this end, 240 samples of peanut products traded in the cities of Araras, Leme, Pirassununga and Porto Ferreira were collected from June 2006 to May 2007. The samples were analyzed f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10010174
更新日期：2009-01-01 00:00:00
abstract：:Antimicrobial peptides have been identified as one of the alternatives to the extensive use of common antibiotics as they show a broad spectrum of activity against human pathogens. Among these is Chionodracine (Cnd), a host-defense peptide isolated from the Antarctic icefish Chionodracohamatus, which belongs to the fa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041401
更新日期：2020-02-19 00:00:00
abstract：:Aquaporins (AQPs) are among the best structural-characterized membrane proteins, fulfilling the role of allowing water flux across cellular membranes. Thus far, 34 single amino acid polymorphisms have been reported in HUMSAVAR for human aquaporins as disease-related. They affect AQP2, AQP5 and AQP8, where they are ass...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19061577
更新日期：2018-05-25 00:00:00
abstract：:Genealogical concordance is a critical overlay of all phylogenetic analyses, irrespective of taxonomic level. To assess such patterns of congruence we have compiled and derived sequence data for two mitochondrial (16S rRNA, COI) and two nuclear (ITS1, p26) markers in 14 American populations of the hypersaline branchio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10125455
更新日期：2009-12-18 00:00:00
abstract：:Transthyretin (TTR) is a homotetrameric protein that circulates in plasma and cerebral spinal fluid (CSF) whose aggregation into amyloid fibrils has been associated with at least two different amyloid diseases: senile systemic amyloidosis (SSA) and familial amyloid polyneuropathy (FAP). In SSA aggregates are composed ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14035284
更新日期：2013-03-06 00:00:00
