Abstract：
:We characterized Schistosoma japonicum HSP40 (Sjp40) and HSP90α (Sjp90α) in this study. Western blot analysis revealed both are present in soluble egg antigens and egg secretory proteins, implicating them in triggering the host immune response after secretion from eggs into host tissues. These observations were confirmed by immunolocalization showing both HSPs are located in the Reynolds' layer within mature eggs, suggesting they are secreted by miracidia and accumulate between the envelope and the eggshell. Both HSPs are present in the musculature and parenchyma of adult males and in the vitelline cells of females; only Sjp90α is present on the tegument of adults. Sjp40 was able to enhance the expression of macrophages, dendritic cells, and eosinophilic cells in mouse liver non-parenchymal cells, whereas rSjp90α only stimulated the expression of dendritic cells. T helper 1 (Th1), Th2, and Th17 responses were increased upon rSjp40 stimulation in vitro, but rSjp90 only stimulated an increased Th17 response. Sjp40 has an important role in reducing the expression of fibrogenic gene markers in hepatic stellate cells in vitro. Overall, these findings provide new information on HSPs in S. japonicum, improving our understanding of the pathological roles they play in their interaction with host immune cells.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Xu Z,Ji M,Li C,Du X,Hu W,McManus DP,You Hdoi
10.3390/ijms21114034subject
Has Abstractpub_date
2020-06-04 00:00:00issue
11issn
1422-0067pii
ijms21114034journal_volume
21pub_type
杂志文章abstract：:Plant NADPH oxidases, formerly known as respiratory burst oxidase homologues (RBOHs), are plasma membrane enzymes dedicated to reactive oxygen species (ROS) production. These oxidases are implicated in a wide variety of processes, ranging from tissue and organ growth and development to signaling pathways in response t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17050680
更新日期：2016-05-18 00:00:00
abstract：:d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear. In this study, using the loperamide-induced constipation Kunming mice as the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20225721
更新日期：2019-11-14 00:00:00
abstract：:There is a growing body of evidence showing the importance of physical activity against acute ischemic events in various organs. Ischemia/reperfusion injury (I/R) is characterized by tissue damage as a result of restriction and subsequent restoration of blood supply to an organ. Oxidative stress due to increased react...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249382
更新日期：2020-12-09 00:00:00
abstract：:The endoplasmic reticulum (ER) is the principal organelle responsible for correct protein folding, a step in protein synthesis that is critical for the functional conformation of proteins. ER stress is a primary feature of secretory cells and is involved in the pathogenesis of numerous human diseases, such as certain ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17020192
更新日期：2016-02-01 00:00:00
abstract：:MicroRNAs (miRNAs) are important post-transcriptional regulators during adipocyte adipogenesis. MiR-206-3p, a tissue-specific miRNA, is absent in white adipocytes. In this study, we examined the roles of mmu-miR-206-3p in the adipogenic differentiation of 3T3-L1 preadipocytes. The miR-206-3p expression has shown an ap...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071510
更新日期：2017-07-14 00:00:00
abstract：:Speckle-type BTB/POZ protein (SPOP) is a substrate recognition receptor of the cullin-3 (CUL3)/RING type ubiquitin E3 complex. To date, approximately 30 proteins have been identified as ubiquitinated substrates of the CUL3/SPOP complex. Pathologically, missense mutations in the substrate-binding domain of SPOP have be...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197293
更新日期：2020-10-02 00:00:00
abstract：:Malignant melanoma is developed from pigment-containing cells, melanocytes, and primarily found on the skin. Malignant melanoma still has a high mortality rate, which may imply a lack of therapeutic agents. Lakoochin A, a compound isolated from Artocarpus lakoocha and Artocarpus xanthocarpus, has an inhibitory functio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092649
更新日期：2018-09-06 00:00:00
abstract：:Several studies show that triple-negative breast cancer (TNBC) patients have the lowest vitamin D concentration among all breast cancer types, suggesting that this vitamin may induce a protective effect against TNBC. This effect of the active metabolite of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D), can be attr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21103670
更新日期：2020-05-23 00:00:00
abstract：:Cyclosporin A (CsA), a calcineurin inhibitor, remain the cornerstone of immunosuppressive regimens, regardless of nephrotoxicity, which depends on the duration of drug exposure. The mechanisms and biomarkers underlying the transition from CsA-induced renal dysfunction to nephrotoxicity deserve better elucidation, and ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15058979
更新日期：2014-05-20 00:00:00
abstract：:Although mitochondrial dysfunction has been implicated in aging, physical function decline, and several age-related diseases, an accessible and affordable measure of mitochondrial health is still lacking. In this study we identified the proteomic signature of muscular mitochondrial oxidative capacity in plasma. In 165...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249540
更新日期：2020-12-15 00:00:00
abstract：:Hypoxia is a common environmental stress factor and is associated with fibrogenesis. Matrix metalloproteinase-2 (MMP-2), produced by hepatic stellate cells (HSCs), plays an important role in liver fibrogenesis. However, inconsistent results have been reported on the impact of hypoxia on MMP-2 expression and activity i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12042434
更新日期：2011-01-01 00:00:00
abstract：:Fused deposit modeling (FDM) 3D printing technology cannot generate scaffolds with high porosity while maintaining good integrity, anatomical-surface detail, or high surface area-to-volume ratio (S/V). Solvent casting and particulate leaching (SCPL) technique generates scaffolds with high porosity and high S/V. Howeve...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010315
更新日期：2020-01-02 00:00:00
abstract：BACKGROUND:Widespread use of prostate specific antigen (PSA) in screening procedures allowed early identification of an increasing number of prostate cancers (PCas), mainly including indolent cancer. Availability of different therapeutic strategies which have a very different impact on the patient's quality of life sug...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041184
更新日期：2020-02-11 00:00:00
abstract：:Peripheral nerve injuries remain a significant source of long lasting morbidity, disability, and economic costs. Much research continues to be performed in areas related to improving the surgical outcomes of peripheral nerve repair. In this review, the physiology of peripheral nerve regeneration and the multitude of e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17091494
更新日期：2016-09-07 00:00:00
abstract：:Regulation of synaptically located ionotropic receptors is thought to be the main mechanism by which anaesthetics cause unconsciousness. An alternative explanation, which has received much less attention, is that of primary anaesthetic disruption of brain metabolism via suppression of mitochondrial proteins. In this p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134703
更新日期：2020-07-01 00:00:00
abstract：:Synthetic organic dyes are widely used in various industrial sectors but are also among the most harmful water pollutants. In the last decade, significant efforts have been made to develop improved materials for the removal of dyes from water, in particular, on nanostructured adsorbent materials. Metal organic framewo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020788
更新日期：2021-01-14 00:00:00
abstract：:Increasing evidence implicates mitochondrial dysfunction in the etiology of Parkinson's disease (PD). Mitochondrial DNA (mtDNA) mutations are considered a possible cause and this mechanism might be shared with the aging process and with other age-related neurodegenerative disorders such as Alzheimer's disease (AD). We...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051758
更新日期：2020-03-04 00:00:00
abstract：:We first demonstrated that long-term increased polyamine (spermine, spermidine, putrescine) intake elevated blood spermine levels in mice and humans, and lifelong consumption of polyamine-rich chow inhibited aging-associated increase in aberrant DNA methylation, inhibited aging-associated pathological changes, and ext...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051254
更新日期：2019-03-12 00:00:00
abstract：:We have previously shown that ritonavir (RTV), a highly active anti-retroviral therapy (HAART) drug, can cause endothelial dysfunction through oxidative stress. Several antioxidants including ginsenoside Rb1, a compound with antioxidant effect, can effectively block this side effect of RTV in endothelial cells. In the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20020294
更新日期：2019-01-12 00:00:00
abstract：:In order to supply new information on the antioxidant function of selected beverages for nutritionists and the general public, total phenolic contents of 51 kinds of herbal and tea infusions made in China were measured by the Folin-Ciocalteu method, and their antioxidant capacities were evaluated using ferric reducing...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12042112
更新日期：2011-01-01 00:00:00
abstract：:Extramammary Paget's disease (EMPD) is a rare skin cancer arising in the anogenital area. Most EMPD tumors remain dormant as in situ lesions, but the outcomes of patients with metastatic EMPD are poor because of the lack of effective systemic therapies. Nectin cell adhesion molecule 4 (NECTIN4) has attracted attention...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165891
更新日期：2020-08-16 00:00:00
abstract：:Staphylococcus aureus AgrC is an important component of the agr quorum-sensing system. AgrC is a membrane-embedded histidine kinase that is thought to act as a sensor for the recognition of environmental signals and the transduction of signals into the cytoplasm. However, the difficulty of expressing and purifying fun...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918470
更新日期：2013-09-06 00:00:00
abstract：:Pompe disease is a glycogen storage disease caused by a deficiency in acid α-glucosidase (GAA), a hydrolase necessary for the degradation of lysosomal glycogen. This deficiency in GAA results in muscle and neuronal glycogen accumulation, which causes respiratory insufficiency. Pompe disease mouse models provide a mean...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21062256
更新日期：2020-03-24 00:00:00
abstract：:Nitric oxide (NO/cyclic guanosine monophosphate (cGMP)-regulated cellular mechanisms are involved in a variety of (patho-) physiological processes. One of the main effector molecules in this system, proteinkinase G (PKG), serves as a molecular switch by phosphorylating different target proteins and thereby turning the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041180
更新日期：2018-04-12 00:00:00
abstract：:Climate change-induced abiotic stress results in crop yield and production losses. These stresses result in changes at the physiological and molecular level that affect the development and growth of the plant. Reactive oxygen species (ROS) is formed at high levels due to abiotic stress within different organelles, lea...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155208
更新日期：2020-07-23 00:00:00
abstract：:Patients harbouring mutations in genes encoding C-type natriuretic peptide (CNP; NPPC) or its receptor guanylyl cyclase B (GC-B, NPR2) suffer from severe growth phenotypes; loss-of-function mutations cause achondroplasia, whereas gain-of-function mutations cause skeletal overgrowth. Although most of the effects of CNP...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22031076
更新日期：2021-01-22 00:00:00
abstract：:The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), pre...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082869
更新日期：2020-04-20 00:00:00
abstract：:An imbalanced T-cell homeostasis plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). Co-stimulatory and co-inhibitory molecules regulate T-cell differentiation, survival, and cytokine production. B- and T-lymphocyte attenuator (BTLA) is a co-inhibitory molecule which negatively regulates...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184505
更新日期：2019-09-11 00:00:00
abstract：:The ATP-sensitive K+ channel (KATP) is involved in hypersensitivity during chronic pain and is presumed to be a downstream target of mu opioid receptors. Multiple subtypes of KATP channels exist in the peripheral and central nervous system and their activity may be inversely correlated to chronic pain phenotypes in ro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092251
更新日期：2019-05-07 00:00:00
abstract：:The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Sevent...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610868
更新日期：2014-06-17 00:00:00
