Abstract:
:Antimicrobial peptides have been identified as one of the alternatives to the extensive use of common antibiotics as they show a broad spectrum of activity against human pathogens. Among these is Chionodracine (Cnd), a host-defense peptide isolated from the Antarctic icefish Chionodracohamatus, which belongs to the family of Piscidins. Previously, we demonstrated that Cnd and its analogs display high antimicrobial activity against ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacterbaumannii, Pseudomonas aeruginosa and Enterobacter species). Herein, we investigate the interactions with lipid membranes of Cnd and two analogs, Cnd-m3 and Cnd-m3a, showing enhanced potency. Using a combination of Circular Dichroism, fluorescence spectroscopy, and all-atom Molecular Dynamics (MD) simulations, we determined the structural basis for the different activity among these peptides. We show that all peptides are predominantly unstructured in water and fold, preferentially as α-helices, in the presence of lipid vesicles of various compositions. Through a series of MD simulations of 400 ns time scale, we show the effect of mutations on the structure and lipid interactions of Cnd and its analogs. By explaining the structural basis for the activity of these analogs, our findings provide structural templates to design minimalistic peptides for therapeutics.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Borocci S,Della Pelle G,Ceccacci F,Olivieri C,Buonocore F,Porcelli Fdoi
10.3390/ijms21041401subject
Has Abstractpub_date
2020-02-19 00:00:00issue
4issn
1422-0067pii
ijms21041401journal_volume
21pub_type
杂志文章abstract::Great progress has been made over the past decade with respect to the application of biotechnology to generate nutritionally improved food crops. Biofortified staple crops such as rice, maize and wheat harboring essential micronutrients to benefit the world's poor are under development as well as new varieties of crop...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms16023895
更新日期:2015-02-11 00:00:00
abstract::Multidrug resistance (MDR), of the innate and acquired types, is one of major problems in treating tumor diseases with a good chance of success. In this review, we examine the key role of nuclear factor-kappa B (NF-κB) to induce MDR in three tumor models characterized precisely by innate or acquired MDR, in particular...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21093070
更新日期:2020-04-27 00:00:00
abstract::Bacterial exopolysaccharides produced by lactic acid bacteria are of increasing interest in the food industry, since they might enhance the technological and functional properties of some edible matrices. In this work, Pediococcus parvulus 2.6, which produces an O2-substituted (1,3)-β-d-glucan exopolysaccharide only s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18071588
更新日期:2017-07-21 00:00:00
abstract::Demands for safe depigmentation compounds are constantly increasing in the pharmaceutical and cosmetic industry, since the numerous relevant compounds reported to date have shown undesirable side effects or low anti-melanogenic effects. In this study, we reported three novel inhibitors of tyrosinase, which is the key ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21093144
更新日期:2020-04-29 00:00:00
abstract::Niemann-Pick disease type C (NPC) is a recessive hereditary disease caused by mutation of the NPC1 or NPC2 gene. It is characterized by abnormality of cellular cholesterol trafficking with severe neuronal and hepatic injury. In this study, we investigated the potential of glycoprotein nonmetastatic melanoma protein B ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010452
更新日期:2021-01-05 00:00:00
abstract::Nosocomial (hospital-acquired) infections remain a serious challenge for health systems. The reason for this lies not only in the local imperfection of medical practices and protocols. The frequency of infection with antibiotic-resistant strains of bacteria is growing every year, both in developed and developing count...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21217839
更新日期:2020-10-22 00:00:00
abstract::MCP-1-induced protein (MCPIP, also known as Zc3h12a or Regnase-1), a newly identified suppressor of cytokine signaling, is expressed in endothelial cells (ECs). To investigate the role of endothelial MCPIP in vascular homeostasis and function, we deleted the MCPIP gene specifically in ECs using the Cre-LoxP system. EC...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010172
更新日期:2019-01-05 00:00:00
abstract::Cinnamaldehyde, a natural preservative that can non-specifically deactivate foodborne pathogens, was successfully incorporated into fish skin gelatin (FSG) solutions and blow spun into uniform nanofibers. The effects of cinnamaldehyde ratios (5-30%, w/w FSG) on physicochemical properties of fiber-forming emulsions (FF...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020618
更新日期:2018-02-22 00:00:00
abstract::Schizophrenia is a major psychiatric disorder that afflicts about 1% of the world's population, falling into the top 10 medical disorders causing disability. Existing therapeutic strategies have had limited success on cognitive impairment and long-term disability and are burdened by side effects. Although new antipsyc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18102044
更新日期:2017-09-22 00:00:00
abstract::Liquid water is considered to be a peculiar example of glass forming materials because of the possibility of giving rise to amorphous phases with different densities and of the thermodynamic anomalies that characterize its supercooled liquid phase. In the present work, literature data on the density of bulk liquid wat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197269
更新日期:2020-10-01 00:00:00
abstract::Lysin-motif receptor-like kinase PsK1 is involved in symbiosis initiation and the maintenance of infection thread (IT) growth and bacterial release in pea. We verified PsK1 specificity in relation to the Nod factor structure using k1 and rhizobial mutants. Inoculation with nodO and nodE nodO mutants significantly redu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071624
更新日期:2019-04-01 00:00:00
abstract::Fusarium wilt (Panama disease) caused by Fusarium oxysporum f. sp. cubense (FOC) represents a significant threat to banana (Musa spp.) production. Musa AAB is susceptible to Race 1 (FOC1) and Race 4 (FOC4), while Cavendish Musa AAA is found to be resistant to FOC1 but still susceptible to Race 4. A polygalacturonase (...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16047595
更新日期:2015-04-03 00:00:00
abstract::Aqueous solutions of isovaline and its precursor molecule, 5-ethyl-5-methylhydantoin, were irradiated with ultraviolet and γ-ray photons, to evaluate their structural stability against space radiation. The degree of photolysis was measured and irradiation products were identified using chiral, reversed-phase and ion-e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13011006
更新日期:2012-01-01 00:00:00
abstract::A complex antioxidant system has been developed in mammals to relieve oxidative stress. However, excessive reactive species derived from oxygen and nitrogen may still lead to oxidative damage to tissue and organs. Oxidative stress has been considered as a conjoint pathological mechanism, and it contributes to initiati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms161125942
更新日期:2015-11-02 00:00:00
abstract::Peripheral nerve injuries remain a significant source of long lasting morbidity, disability, and economic costs. Much research continues to be performed in areas related to improving the surgical outcomes of peripheral nerve repair. In this review, the physiology of peripheral nerve regeneration and the multitude of e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17091494
更新日期:2016-09-07 00:00:00
abstract:BACKGROUND:A growing body of data indicates that the physiology of the liver is sex-hormone dependent, with some types of liver failure occurring more frequently in males, and some in females. In males, in physiological conditions, testosterone acts via androgen receptors (AR) to increase insulin receptor (IR) expressi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031242
更新日期:2021-01-27 00:00:00
abstract::Articular cartilage is a structure lack of vascular distribution. Once the cartilage is injured or diseased, it is unable to regenerate by itself. Surgical treatments do not effectively heal defects in articular cartilage. Tissue engineering is the most potential solution to this problem. In this study, methoxy poly(e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041125
更新日期:2018-04-09 00:00:00
abstract::Stem/progenitor cells are promising candidates for the regeneration of parenchyma in acute and chronic renal failure. However, recent data exhibit that survival of stem/progenitor cells after implantation in diseased renal parenchyma is restricted. To elaborate basic parameters improving survival, cell seeding was sim...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151223240
更新日期:2014-12-15 00:00:00
abstract::It has recently been recognized that inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), upregulate the secretion of matrix metalloproteinase-9 (MMP-9) from cancer cells and thereby promote peritoneal dissemination. In this study, we found that TNF-α also stimulated peritoneal mesothelial cells to secrete...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123961
更新日期:2018-12-09 00:00:00
abstract::Abiotic stresses are major constraints limiting crop growth and production. Heat shock factors (Hsfs) play significant roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. In this study, we explored the biological functions and underlying mechanisms of wheat TaHs...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21093121
更新日期:2020-04-28 00:00:00
abstract::Wound healing is a physiological process, involving three successive and overlapping phases-hemostasis/inflammation, proliferation, and remodeling-to maintain the integrity of skin after trauma, either by accident or by procedure. Any disruption or unbalanced distribution of these processes might result in abnormal wo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18112325
更新日期:2017-11-03 00:00:00
abstract::Following herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies' fitness too. In tomato there is still a lack of information on the genetic bases res...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092748
更新日期:2018-09-13 00:00:00
abstract::Therapeutic drug monitoring is routinely performed to maintain optimal tacrolimus concentrations in kidney transplant recipients. Nonetheless, toxicity and rejection still occur within an acceptable concentration-range. To have a better understanding of the relationship between tacrolimus dose, tacrolimus concentratio...
journal_title:International journal of molecular sciences
pub_type: 临床试验,杂志文章
doi:10.3390/ijms20194710
更新日期:2019-09-23 00:00:00
abstract:BACKGROUND:Dp71 is the most abundant dystrophin (DMD) gene product in the nervous system. Mutation in the Dp71 coding region is associated with cognitive disturbances in Duchenne muscular dystrophy (DMD) patients, but the function of dystrophin Dp71 in tumor progression remains to be established. This study investigate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215429
更新日期:2019-10-31 00:00:00
abstract::Copper-containing coordination compounds attract wide attention due to the redox activity and biogenicity of copper ions, providing multiple pathways of biological activity. The pharmacological properties of metal complexes can be fine-tuned by varying the nature of the ligand and donor atoms. Copper-containing coordi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21113965
更新日期:2020-05-31 00:00:00
abstract::Although platelet-rich fibrin (PRF) has been used in clinical practice for some time, to date, few studies reveal its role as a bioactive scaffold in facilitating meniscal repair. Here, the positive anabolic effects of PRF on meniscocytes harvested from the primary culture of a rabbit meniscus were revealed. The rabbi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081722
更新日期:2017-08-07 00:00:00
abstract:BACKGROUND:Although it has been previously demonstrated that acute inflammation can promote the tumor growth of a sub-tumorigenic dose of melanoma cells through of 5-lipoxygenase inflammatory pathway and its product leukotriene B4, and also that the peritumoral treatment with eicosapentaenoic acid and its product, leuk...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20153765
更新日期:2019-08-01 00:00:00
abstract::Osteoporosis is a common skeletal disorder, occurring as a result of an imbalance between bone resorption and bone formation, with bone breakdown exceeding bone building. Bone resorption inhibitors, e.g., bisphosphonates, have been designed to treat osteoporosis. Teriparatide, an anabolic agent, stimulates bone format...
journal_title:International journal of molecular sciences
pub_type: 社论
doi:10.3390/ijms21218005
更新日期:2020-10-28 00:00:00
abstract::Translation is one of the final steps that regulate gene expression. The ribosome is the effector of translation through to its role in mRNA decoding and protein synthesis. Many mechanisms have been extensively described accounting for translational regulation. However it emerged only recently that ribosomes themselve...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20051226
更新日期:2019-03-11 00:00:00
abstract::The current work reports the synthesis, spectroscopic studies, antiradical and antiproliferative properties of four ruthenium(III) complexes of heterocyclic tridentate Schiff base bearing a simple 2',4'-dihydroxyacetophenone functionality and ethylenediamine as the bridging ligand with RCHO moiety. The reaction of the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17010060
更新日期:2016-01-04 00:00:00