Abstract：
:In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed FRET from QDs donor to DyLight acceptor, which were covalently conjugated with human IgG and goat anti-human IgG, respectively. The immunocomplex was formed and the mutual affinity of the antigen and antibody brought QDs and DyLight close enough to allow FRET to occur. This novel CE-based technique can be easily extended to other FRET systems based on QDs and may have potential application in the detection of antibodies.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wang J,Qiu L,Wang C,Zhang Y,Li J,Xia J,Jiang Pdoi
10.3390/ijms140919146subject
Has Abstractpub_date
2013-09-17 00:00:00pages
19146-54issue
9issn
1422-0067pii
ijms140919146journal_volume
14pub_type
杂志文章abstract：:Tauroursodeoxycholic acid (TUDCA) is a US FDA-approved hydrophilic bile acid for the treatment of chronic cholestatic liver disease. In the present study, we investigate the effects of TUDCA on the proliferation and differentiation of osteoblasts and its therapeutic effect on a mice model of osteoporosis. Following tr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124274
更新日期：2020-06-16 00:00:00
abstract：:Plants infected with DNA viruses produce massive quantities of virus-derived, 24-nucleotide short interfering RNAs (siRNAs), which can potentially direct viral DNA methylation and transcriptional silencing. However, growing evidence indicates that the circular double-stranded DNA accumulating in the nucleus for Pol II...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140815233
更新日期：2013-07-24 00:00:00
abstract：:Alzheimer's disease (AD) is a progressive neurodegenerative disease. In this study, to investigate the effect of microglial elimination on AD progression, we administered PLX3397, a selective colony-stimulating factor 1 receptor inhibitor, to the mouse model of AD (5xFAD mice). Amyloid-beta (Aβ) deposition and amyloid...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155553
更新日期：2020-08-03 00:00:00
abstract：:The authors describe a new type of titanium (Ti) implant as a Modi-anodized (ANO) Ti implant, the surface of which was treated by sandblasting, acid etching (SLA), and anodized techniques. The aim of the present study was to evaluate the adhesion of MG-63 cells to Modi-ANO surface treated Ti in vitro and to investigat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160510324
更新日期：2015-05-06 00:00:00
abstract：:miR-29a is a conserved miRNA that participates in bone formation and immune response in vertebrates. miR-29a of Pinctada martensii (Pm-miR-29a) was identified in the previous research though deep sequencing. In this report, the precise sequence of mature Pm-miR-29a was validated using miRNA rapid amplification of cDNA...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226182
更新日期：2015-12-10 00:00:00
abstract：:Drug delivery nanocarriers, especially targeted drug delivery by liposomes are emerging as a class of therapeutics for cancer. Early research results suggest that liposomal therapeutics enhanced efficacy, while simultaneously reducing side effects, owing to properties such as more targeted localization in tumors and a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms16010230
更新日期：2014-12-24 00:00:00
abstract：:Sperm cryopreservation and artificial insemination are important methods for giant panda breeding and preservation of extant genetic diversity. Lower conception rates limit the use of artificial insemination with frozen-thawed giant panda sperm, due to the lack of understanding of the cryodamaging or cryoinjuring mech...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19103066
更新日期：2018-10-08 00:00:00
abstract：:Autophagy is a general protective mechanism for maintaining homeostasis in eukaryotic cells, regulating cellular metabolism, and promoting cell survival by degrading and recycling cellular components under stress conditions. The degradation pathway that is mediated by autophagy receptors is called selective autophagy,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21062008
更新日期：2020-03-16 00:00:00
abstract：:Hemostasis disorder in patients with end-stage renal disease (ESRD) is frequently associated with bleeding diathesis but it may also manifest in thrombotic complications. Analysis of individual coagulation and fibrinolytic factors may shed light on the background of this paradox situation. Here we explored components ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228426
更新日期：2020-11-10 00:00:00
abstract：:Tuberculosis (TB) is a chronic infectious disease in which prolonged, non-resolutive inflammation of the lung may lead to metabolic and neuroendocrine dysfunction. Previous studies have reported that individuals coursing pulmonary TB experience cognitive or behavioural changes; however, the pathogenic substrate of suc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249483
更新日期：2020-12-13 00:00:00
abstract：:Gastric cancer (GC) is a deadly disease with poor prognosis that is characterized by heterogeneity. New classifications based on histologic features, genotypes, and molecular phenotypes, for example, the Cancer Genome Atlas subtypes and those by the Asian Cancer Research Group, help understand the carcinogenic differe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249400
更新日期：2020-12-10 00:00:00
abstract：:One of the hallmarks of the acute toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a drastically reduced feed intake by an unknown mechanism. To further elucidate this wasting syndrome, we followed the effects of a single large dose (100 μg/kg) of TCDD on the serum levels of several energy balance-influencing...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150813938
更新日期：2014-08-12 00:00:00
abstract：:Lifestyle-related disorders, such as the metabolic syndrome, have become a primary risk factor for the development of liver pathologies that can progress from hepatic steatosis, hepatic insulin resistance, steatohepatitis, fibrosis and cirrhosis, to the most severe condition of hepatocellular carcinoma (HCC). While th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19030921
更新日期：2018-03-20 00:00:00
abstract：:Bacteria colonize most of the human body, and the female genital tract is not an exception. While the existence of a vaginal microbiota has been well established, the upper genital tract has been considered a sterile environment, with a general assumption that bacterial presence is associated with adverse clinical man...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20143405
更新日期：2019-07-11 00:00:00
abstract：:Drought is one of the major environmental factors limiting biomass and seed yield production in agriculture. In this research, we focused on plants from the Fabaceae family, which has a unique ability for the establishment of symbiosis with nitrogen-fixing bacteria, and are relatively susceptible to water limitation. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051204
更新日期：2019-03-09 00:00:00
abstract：:Melanin is an important phenolic skin pigment found throughout the animal kingdom. Tyrosine and its hydroxylated product dopa provide the starting material for melanin biosynthesis in all animals. Through a set of well-established reactions, they are converted to 5,6-dihydroxyindole (DHI) and DHI-2-carboxylic acid (DH...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197321
更新日期：2020-10-03 00:00:00
abstract：:The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, accord...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11041458
更新日期：2010-04-02 00:00:00
abstract：:Cancer represents a group of heterogeneous diseases characterized by uncontrolledgrowth and spread of abnormal cells, ultimately leading to death. Nanomedicine plays a significantrole in the development of nanodrugs, nanodevices, drug delivery systems and nanocarriers. Someof the major issues in the treatment of cance...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103264
更新日期：2018-10-20 00:00:00
abstract：:Early-life adversity may have programming effects on neuroendocrine and immune adaptation mechanisms in humans and socially living animals. Using a pig model, we investigated the effect of daily 2-h maternal and littermate deprivation from postnatal days 2-15, either alone (DA) or in a group of littermates (DG) on the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155212
更新日期：2020-07-23 00:00:00
abstract：:Thioredoxin fold proteins (TFPs) form a family of diverse proteins involved in thiol/disulfide exchange in cells from all domains of life. Leptospirillum spp. are bioleaching bacteria naturally exposed to extreme conditions like acidic pH and high concentrations of metals that can contribute to the generation of react...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051880
更新日期：2020-03-10 00:00:00
abstract：:In this paper, we investigated the activation energies of the aggregation−disaggregation self-oscillation induced by the Belousov-Zhabotinsky (BZ) reaction by utilizing the nonthermoresponsive polymer chain in a wide temperature range. This is because the conventional type self-oscillating polymer chain, with thermore...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131216281
更新日期：2012-12-03 00:00:00
abstract：:This study investigated the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-modified glass ionomer cement (GIC) on shear bond strength (SBS) and remineralisation of artificial "caries-affected" dentine. Human dentine slices were demineralised and allocated to three groups: group 1, conventional G...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081723
更新日期：2017-08-07 00:00:00
abstract：:The molecular mechanism regulating embryo development under reduced oxygen tension remains elusive. This study aimed to identify the molecular mechanism impacting embryo development under low oxygen conditions. Buffalo embryos were cultured under 5% or 20% oxygen and were evaluated according to their morphological par...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21061996
更新日期：2020-03-14 00:00:00
abstract：:Liver fibrosis is a complex process associated to most types of chronic liver disease, which is characterized by a disturbance of hepatic tissue architecture and the excessive accumulation of extracellular matrix. Resolvin E1 (RvE1) is a representative member of the eicosapentaenoic omega-3 lipid derivatives, and is a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228827
更新日期：2020-11-22 00:00:00
abstract：:Inflammatory bowel disease (IBD) is a chronic disorder manifested as Crohn's disease (CD) and ulcerative colitis (UC) characterized by intestinal inflammation and involves a dysregulated immune response against commensal microbiota through the activation of CD4 T helper cells. T helper cell differentiation to effector...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21093379
更新日期：2020-05-11 00:00:00
abstract：:Platelet-released growth factor (PRGF) is a thrombocyte concentrate lysate which, like its clinically equivalent variations (e.g., Vivostat PRF® (platelet-rich fibrin)), is known to support the healing of chronic and hard-to-heal wounds. However, studies on the effect of PRGF on keratinocytes remain scarce. This study...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124404
更新日期：2020-06-20 00:00:00
abstract：:Relaxin is known to play an important role in animal pregnancies, including those of humans. It is suggested that relaxin induces aggressive cell growth and invasiveness in several types of cancer, including endometrial cancer. However, the mechanisms of relaxin remain largely unclear. In this study, we examined the e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082438
更新日期：2018-08-18 00:00:00
abstract：:miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In this region, many key ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17040559
更新日期：2016-04-14 00:00:00
abstract：:Heterosis has been widely accepted as an effective strategy to increase yields in plant breeding. Notably, the chemical hybridization agent SQ-1 induces male sterility in wheat, representing a critical potential tool in hybrid seed production. However, the mechanisms underlying the male sterility induced by SQ-1 still...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072468
更新日期：2020-04-02 00:00:00
abstract：:Long-standing efforts to identify the multifaceted roles of histone deacetylase inhibitors (HDACis) have positioned these agents as promising drug candidates in combatting cancer, autoimmune, neurodegenerative, and infectious diseases. The same has also encouraged the evaluation of multiple HDACi candidates in preclin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20092241
更新日期：2019-05-07 00:00:00
