Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae.

Abstract:

:The values of physiological indices and the enzymes activities involved in the overwintering stage were studied in D. armandi larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before declining until the end of the winter. The activities of four enzymes (SOD, CAT, LDH and AchE) decreased, whereas POD, PK and MDH showed opposite trends in activity. Other enzyme activities (those of TPS, SDH and GLK) were low during the overwintering period and later increased and stabilized during spring. In this study, a polymerase chain reaction (PCR) genes of SDH, TPS and GLK was utilized to identify DarmSDH, DarmTPS and DarmGLK in D. armandi. They were found to be abundantly expressed during the overwintering stage by quantitative real-time PCR (qRT-PCR) analyses; by contrast, these three genes showed higher expression levels in December 2014 than in May 2015. The qRT-PCR results demonstrated that the reduction of mRNA expression levels was significant in DarmSDH-, DarmTPS- and DarmGLK-dsRNA-treated D. armandi compared with water-injected and non-injected controls. The mortality responses at low temperature were also increased in the dsRNA-treated D. armandi compared with the controls.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Wang J,Zhang RR,Gao GQ,Ma MY,Chen H

doi

10.1038/srep34698

subject

Has Abstract

pub_date

2016-10-05 00:00:00

pages

34698

issn

2045-2322

pii

srep34698

journal_volume

6

pub_type

杂志文章
  • Relating significance and relations of differentially expressed genes in response to Aspergillus flavus infection in maize.

    abstract::Aspergillus flavus is a pathogenic fungus infecting maize and producing aflatoxins that are health hazards to humans and animals. Characterizing host defense mechanism and prioritizing candidate resistance genes are important to the development of resistant maize germplasm. We investigated methods amenable for the ana...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04815

    authors: Asters MC,Williams WP,Perkins AD,Mylroie JE,Windham GL,Shan X

    更新日期:2014-04-28 00:00:00

  • Comprehensive quantification of triacylglycerols in soybean seeds by electrospray ionization mass spectrometry with multiple neutral loss scans.

    abstract::Soybean seeds are an important source of vegetable oil and biomaterials. The content of individual triacylglycerol species (TAG) in soybean seeds is difficult to quantify in an accurate and rapid way. The present study establishes an approach to quantify TAG species in soybean seeds utilizing an electrospray ionizatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06581

    authors: Li M,Butka E,Wang X

    更新日期:2014-10-10 00:00:00

  • Regional nutrient decrease drove redox stabilisation and metazoan diversification in the late Ediacaran Nama Group, Namibia.

    abstract::The late Ediacaran witnessed an increase in metazoan diversity and ecological complexity, marking the inception of the Cambrian Explosion. To constrain the drivers of this diversification, we combine redox and nutrient data for two shelf transects, with an inventory of biotic diversity and distribution from the Nama G...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59335-2

    authors: Bowyer FT,Shore AJ,Wood RA,Alcott LJ,Thomas AL,Butler IB,Curtis A,Hainanan S,Curtis-Walcott S,Penny AM,Poulton SW

    更新日期:2020-02-10 00:00:00

  • HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver.

    abstract::Adaptive responses to hypoxia regulate hepatic lipid metabolism, but their consequences in nonalcoholic fatty liver disease (NAFLD) are largely unknown. Here, we show that hypoxia inducible factor-1 (HIF-1), a key determinant of hypoxic adaptations, prevents excessive hepatic lipid accumulation in the progression of N...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32586-w

    authors: Arai T,Tanaka M,Goda N

    更新日期:2018-09-21 00:00:00

  • Converse Magnetoelectric Composite Resonator for Sensing Small Magnetic Fields.

    abstract::Magnetoelectric (ME) thin film composites consisting of sputtered piezoelectric (PE) and magnetostrictive (MS) layers enable for measurements of magnetic fields passively, i.e. an AC magnetic field directly generates an ME voltage by mechanical coupling of the MS deformation to the PE phase. In order to achieve high f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52657-w

    authors: Hayes P,Jovičević Klug M,Toxværd S,Durdaut P,Schell V,Teplyuk A,Burdin D,Winkler A,Weser R,Fetisov Y,Höft M,Knöchel R,McCord J,Quandt E

    更新日期:2019-11-08 00:00:00

  • Pharmacologic ATM but not ATR kinase inhibition abrogates p21-dependent G1 arrest and promotes gastrointestinal syndrome after total body irradiation.

    abstract::We show that ATM kinase inhibition using AZ31 prior to 9 or 9.25 Gy total body irradiation (TBI) reduced median time to moribund in mice to 8 days. ATR kinase inhibition using AZD6738 prior to TBI did not reduce median time to moribund. The striking finding associated with ATM inhibition prior to TBI was increased cry...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41892

    authors: Vendetti FP,Leibowitz BJ,Barnes J,Schamus S,Kiesel BF,Abberbock S,Conrads T,Clump DA,Cadogan E,O'Connor MJ,Yu J,Beumer JH,Bakkenist CJ

    更新日期:2017-02-01 00:00:00

  • Protease Inhibition Improves Healing of The Vaginal Wall after Obstetrical Injury: Results from a Preclinical Animal Model.

    abstract::Vaginal delivery with obstetrical trauma is a risk factor for pelvic organ prolapse later in life. Loss of fibulin-5 (FBLN5), an elastogenesis-promoting cellular matrix protein, results in prolapse in mice. Here, we evaluated effects of pregnancy, parturition, and obstetrical injury on FBLN5 content, elastic fibers, b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63031-6

    authors: Hamner J,Florian-Rodriguez M,Acevedo J,Shi H,Word RA

    更新日期:2020-04-14 00:00:00

  • Pre-symptomatic modified phytohormone profile is associated with lower phytoplasma titres in an Arabidopsis seor1ko line.

    abstract::The proteins AtSEOR1 and AtSEOR2 occur as conjugates in the form of filaments in sieve elements of Arabidopsis thaliana. A reduced phytoplasma titre found in infected defective-mutant Atseor1ko plants in previous work raised the speculation that non-conjugated SEOR2 is involved in the phytohormone-mediated suppression...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71660-0

    authors: Bernardini C,Pagliari L,De Rosa V,Almeida-Trapp M,Santi S,Martini M,Buoso S,Loschi A,Loi N,Chiesa F,Mithöfer A,van Bel AJE,Musetti R

    更新日期:2020-09-08 00:00:00

  • Rattlesnakes are extremely fast and variable when striking at kangaroo rats in nature: Three-dimensional high-speed kinematics at night.

    abstract::Predation plays a central role in the lives of most organisms. Predators must find and subdue prey to survive and reproduce, whereas prey must avoid predators to do the same. The resultant antagonistic coevolution often leads to extreme adaptations in both parties. Few examples capture the imagination like a rapid str...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40412

    authors: Higham TE,Clark RW,Collins CE,Whitford MD,Freymiller GA

    更新日期:2017-01-13 00:00:00

  • Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere.

    abstract::Chloromethane (CH3Cl) is a toxic gas mainly produced naturally, in particular by plants, and its emissions contribute to ozone destruction in the stratosphere. Conversely, CH3Cl can be degraded and used as the sole carbon and energy source by specialised methylotrophic bacteria, isolated from a variety of environments...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17421-y

    authors: Farhan Ul Haque M,Besaury L,Nadalig T,Bringel F,Mutterer J,Schaller H,Vuilleumier S

    更新日期:2017-12-14 00:00:00

  • A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body.

    abstract::Bacteria swim in sequences of straight runs that are interrupted by turning events. They drive their swimming locomotion with the help of rotating helical flagella. Depending on the number of flagella and their arrangement across the cell body, different run-and-turn patterns can be observed. Here, we present fluoresc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16428-9

    authors: Hintsche M,Waljor V,Großmann R,Kühn MJ,Thormann KM,Peruani F,Beta C

    更新日期:2017-12-01 00:00:00

  • Dyschloremia is associated with failure to restore renal function in survivors with acute kidney injury: an observation retrospective study.

    abstract::Dyschloremia is common in critically ill patients. However, little is known about the effects of dyschloremia on renal function in patients with acute kidney injury (AKI) requiring continuous renal replacement therapy (CRRT). A total of 483 patients who received CRRT for AKI were selected and divided into three groups...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76798-5

    authors: Kee YK,Jeon HJ,Oh J,Shin DH

    更新日期:2020-11-12 00:00:00

  • Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing.

    abstract::We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49445-x

    authors: Xue VW,Cheung MT,Chan PT,Luk LLY,Lee VH,Au TC,Yu AC,Cho WCS,Tsang HFA,Chan AK,Wong SCC

    更新日期:2019-09-10 00:00:00

  • Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the AltSB locus.

    abstract::Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, AltSB, and increases grain yield under Al toxicity. Here, AltSB loci associated with Al tolerance were co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27817-z

    authors: Hufnagel B,Guimaraes CT,Craft EJ,Shaff JE,Schaffert RE,Kochian LV,Magalhaes JV

    更新日期:2018-07-04 00:00:00

  • Numerical analysis of wide-field optical imaging with a sub-20 nm resolution based on a meta-sandwich structure.

    abstract::Biological research requires wide-field optical imaging techniques with resolution down to the nanometer scale to study the biological process in a sub-cell or single molecular level. To meet this requirement, wide-field structured illumination method (WFSIM) has been extensively studied. The resolution of WFSIM is de...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01521-w

    authors: Cao S,Wang T,Yang J,Hu B,Levy U,Yu W

    更新日期:2017-05-02 00:00:00

  • Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan.

    abstract::Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA dou...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58746-5

    authors: Belmans N,Gilles L,Vermeesen R,Virag P,Hedesiu M,Salmon B,Baatout S,Lucas S,Lambrichts I,Jacobs R,Moreels M,DIMITRA Research Group.

    更新日期:2020-02-07 00:00:00

  • An African loss-of-function CACNA1C variant p.T1787M associated with a risk of ventricular fibrillation.

    abstract::Calcium regulation plays a central role in cardiac function. Several variants in the calcium channel Cav1.2 have been implicated in arrhythmic syndromes. We screened patients with Brugada syndrome, short QT syndrome, early repolarisation syndrome, and idiopathic ventricular fibrillation to determine the frequency and ...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/s41598-018-32867-4

    authors: Blancard M,Debbiche A,Kato K,Cardin C,Sabrina G,Gandjbakhch E,Probst V,Haissaguerre M,Extramiana F,Hocini M,Olivier G,Leenhardt A,Guicheney P,Rougier JS

    更新日期:2018-10-02 00:00:00

  • Crotoxin promotes macrophage reprogramming towards an antiangiogenic phenotype.

    abstract::Crotoxin (CTX) is the primary toxin of South American rattlesnake Crotalus durissus terrificus venom. CTX reduces tumour mass, and tumour cell proliferation and these effects seem to involve the formation of new vessels. Angiogenesis has a key role in tumour growth and progression and is regulated by macrophage secret...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40903-0

    authors: de Araújo Pimenta L,de Almeida MES,Bretones ML,Cirillo MC,Curi R,Sampaio SC

    更新日期:2019-03-12 00:00:00

  • Evaluating novel synthetic compounds active against Bacillus subtilis and Bacillus cereus spores using Live imaging with SporeTrackerX.

    abstract::An empirical approach was taken to screen a novel synthetic compound library designed to be active against Gram-positive bacteria. We obtained five compounds that were active against spores from the model organism Bacillus subtilis and the food-borne pathogen Bacillus cereus during our population based experiments. Us...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27529-4

    authors: Omardien S,Ter Beek A,Vischer N,Montijn R,Schuren F,Brul S

    更新日期:2018-06-14 00:00:00

  • Crystallographic anisotropy of the resistivity size effect in single crystal tungsten nanowires.

    abstract::This work demonstrates an anisotropic increase in resistivity with decreasing width in single crystal tungsten (W) nanowires having a height of 21 nm. Nanowire-widths were in the range of 15-451 nm, with the anisotropy observed for widths below 50 nm. The longitudinal directions of the nanowires coincided with the <10...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02591

    authors: Choi D,Moneck M,Liu X,Oh SJ,Kagan CR,Coffey KR,Barmak K

    更新日期:2013-01-01 00:00:00

  • Peripheral perfusion, measured by perfusion index, is a novel indicator for renal events in patients with type 2 diabetes mellitus.

    abstract::Diabetic kidney disease (DKD) is one of the leading causes of end stage renal disease. Despite recent therapies, mortality due to DKD and resources spent on healthcare are important problems. Thus, appropriate markers are needed to predict renal outcomes. Therefore, we investigated the role of peripheral perfusion as ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62926-8

    authors: Okada H,Tanaka M,Yasuda T,Okada Y,Norikae H,Fujita T,Nishi T,Oyamada H,Yamane T,Fukui M

    更新日期:2020-04-08 00:00:00

  • BRCA1 and BRCA2 genes mutations among high risk breast cancer patients in Jordan.

    abstract::Familial breast cancer is estimated to account for 15-20% of all cases of breast cancer. Surveillance for familial breast cancer is well-established world-wide. However, this service does not exist in Jordan, due to the scarcity of information with regard to the genetic profiling of these patients, and therefore lack ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74250-2

    authors: Abu-Helalah M,Azab B,Mubaidin R,Ali D,Jafar H,Alshraideh H,Drou N,Awidi A

    更新日期:2020-10-16 00:00:00

  • Dynamical Analysis of Standing Balance Control on Sloped Surfaces in Individuals with Lumbar Disc Herniation.

    abstract::The changes of balance control mechanism caused by lumbar disc herniation (LDH) has not been well understood. This study aimed to investigate the effects of LDH on the balance control during standing on sloped surfaces. Ten patients with LDH and 10 gender- and age-matched healthy subjects were instructed to stand quie...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58455-z

    authors: Li J,Zhang Y,Song S,Hou Y,Hong Y,Yue S,Li K

    更新日期:2020-02-03 00:00:00

  • Author Correction: How visual experience impacts the internal and external spatial mapping of sensorimotor functions.

    abstract::A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41598-017-16992-0

    authors: Crollen V,Albouy G,Lepore F,Collignon O

    更新日期:2017-12-04 00:00:00

  • Reproductive performance of gilthead seabream (Sparus aurata) broodstock showing different expression of fatty acyl desaturase 2 and fed two dietary fatty acid profiles.

    abstract::Previous studies have shown that it is possible to nutritionally program gilthead seabream offspring through fish oil (FO) replacement by vegetable oils (VO) in the broodstock diet, to improve their ability to grow fast when fed low fish meal (FM) and FO diets during grow-out phase. However, in those studies broodstoc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72166-5

    authors: Ferosekhan S,Xu H,Turkmen S,Gómez A,Afonso JM,Fontanillas R,Rosenlund G,Kaushik S,Izquierdo M

    更新日期:2020-09-23 00:00:00

  • Use of Ultrasonic Device in Cervical and Thoracic Laminectomy: a Retrospective Comparative Study and Technical Note.

    abstract::Multilevel severe compressive myelopathy is a challenging disorder for the surgeons, the aim of this study is to assess the efficacy and safety of a newly designed ultrasonic burr as an assistant tool to the ultrasonic scalpel in laminectomy for this disease. This is a retrospective comparative study, the included sub...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22454-y

    authors: Chen Y,Chang Z,Yu X,Song R,Huang W

    更新日期:2018-03-05 00:00:00

  • EnSVMB: Metagenomics Fragments Classification using Ensemble SVM and BLAST.

    abstract::Metagenomics brings in new discoveries and insights into the uncultured microbial world. One fundamental task in metagenomics analysis is to determine the taxonomy of raw sequence fragments. Modern sequencing technologies produce relatively short fragments and greatly increase the number of fragments, and thus make th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09947-y

    authors: Jiang Y,Wang J,Xia D,Yu G

    更新日期:2017-08-25 00:00:00

  • Rectifying properties of oligo(phenylene ethynylene) heterometallic molecular junctions: molecular length and side group effects.

    abstract::The rectifying properties of α,ω-dithiol terminated oligo(phenylene ethynylene) molecules sandwiched between heterometallic electrodes, including the molecular length and side group effects, are theoretically investigated using the fully self-consistent nonequilibrium Green's function method combined with density func...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06357

    authors: Fu XX,Zhang RQ,Zhang GP,Li ZL

    更新日期:2014-09-15 00:00:00

  • Differential effects of spinal motor neuron-derived and skeletal muscle-derived Rspo2 on acetylcholine receptor clustering at the neuromuscular junction.

    abstract::We recently reported that R-spondin 2 (Rspo2), a secreted activator of Wnt/β-catenin signaling, promotes acetylcholine receptor (AChR) clustering and neuromuscular junction (NMJ) formation via its receptor, Lgr5. Rspo2 is expressed highly in spinal motor neurons (SMNs) and marginally in the skeletal muscle, but the or...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31949-7

    authors: Li J,Ito M,Ohkawara B,Masuda A,Ohno K

    更新日期:2018-09-11 00:00:00

  • Metabolomics-based analysis of miniature flask contents identifies tobacco mixture use among the ancient Maya.

    abstract::A particular type of miniature ceramic vessel locally known as "veneneras" is occasionally found during archaeological excavations in the Maya Area. To date, only one study of a collection of such containers successfully identified organic residues through coupled chromatography-mass spectrometry methods. That study i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81158-y

    authors: Zimmermann M,Brownstein KJ,Pantoja Díaz L,Ancona Aragón I,Hutson S,Kidder B,Tushingham S,Gang DR

    更新日期:2021-01-15 00:00:00