Relating significance and relations of differentially expressed genes in response to Aspergillus flavus infection in maize.

Abstract:

:Aspergillus flavus is a pathogenic fungus infecting maize and producing aflatoxins that are health hazards to humans and animals. Characterizing host defense mechanism and prioritizing candidate resistance genes are important to the development of resistant maize germplasm. We investigated methods amenable for the analysis of the significance and relations among maize candidate genes based on the empirical gene expression data obtained by RT-qPCR technique from maize inbred lines. We optimized a pipeline of analysis tools chosen from various programs to provide rigorous statistical analysis and state of the art data visualization. A network-based method was also explored to construct the empirical gene expression relational structures. Maize genes at the centers in the network were considered as important candidate genes for maize DNA marker studies. The methods in this research can be used to analyze large RT-qPCR datasets and establish complex empirical gene relational structures across multiple experimental conditions.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Asters MC,Williams WP,Perkins AD,Mylroie JE,Windham GL,Shan X

doi

10.1038/srep04815

subject

Has Abstract

pub_date

2014-04-28 00:00:00

pages

4815

issn

2045-2322

pii

srep04815

journal_volume

4

pub_type

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