Role of catalase overproduction in drug resistance and virulence in Candida albicans.

Abstract:

AIM:To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS:Strains overproducing the CAT1 gene were constructed. RESULTS:Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen species generated by amphotericin B or ciclopirox olamine. CAT1 overproduction did not change the minimum inhibitory concentration of fungal cells to fungistatic or fungicidal azoles nor to amphotericin B although increased twofold the minimum inhibitory concentration to caspofungin. The role of Cat1 overproduction in virulence and colonization was also analyzed in mouse models. CONCLUSION:The overproduction of Cat1 protects against oxidants, phagocytes and certain antifungals at subinhibitory concentration but does not increase virulence in a systemic infection mouse model.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Román E,Prieto D,Martin R,Correia I,Mesa Arango AC,Alonso-Monge R,Zaragoza O,Pla J

doi

10.2217/fmb-2016-0067

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

1279-1297

eissn

1746-0913

issn

1746-0921

journal_volume

11

pub_type

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