Rigidity of silicone substrates controls cell spreading and stem cell differentiation.

Abstract:

:The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show that harder gels are more osteogenic, softer gels are more adipogenic, and cell spreading areas increase with the silicone gel substrate rigidity. The results of our study indicate that substrate rigidity induces some universal cellular responses independently of the porosity or topography of the substrate.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Vertelov G,Gutierrez E,Lee SA,Ronan E,Groisman A,Tkachenko E

doi

10.1038/srep33411

subject

Has Abstract

pub_date

2016-09-21 00:00:00

pages

33411

issn

2045-2322

pii

srep33411

journal_volume

6

pub_type

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