Abstract:
:A histoanatomical context is imperative in an analysis of gene expression in a cell in a tissue to elucidate physiological function of the cell. In this study, we made technical advances in fluorescence laser microdissection (LMD) in combination with the absolute quantification of small amounts of mRNAs from a region of interest (ROI) in fluorescence-labeled tissue sections. We demonstrate that our fluorescence LMD-RTqPCR method has three orders of dynamic range, with the lower limit of ROI-size corresponding to a single cell. The absolute quantification of the expression levels of the immediate early genes in an ROI equivalent to a few hundred neurons in the hippocampus revealed that mice transferred from their home cage to a novel environment have distinct activation profiles in the hippocampal regions (CA1, CA3, and DG) and that the gene expression pattern in CA1, but not in the other regions, follows a power law distribution.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yoshioka W,Endo N,Kurashige A,Haijima A,Endo T,Shibata T,Nishiyama R,Kakeyama M,Tohyama Cdoi
10.1038/srep00783subject
Has Abstractpub_date
2012-01-01 00:00:00pages
783issn
2045-2322journal_volume
2pub_type
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