Abstract：
:Success of immunotherapeutic approaches using genetically engineered antibodies and T cells modified with chimeric antigen receptors (CARs) depends, among other things, on the selection of antigen binding domains with desirable expression and binding characteristics. We developed a luciferase-based assay, termed Malibu-Glo Assay, which streamlines the process of optimization of an antigen binding domain with desirable properties and allows the sensitive detection of tumor antigens. The assay involves a recombinant immunoconjugate, termed Malibu-Glo reagent, comprising an immunoglobulin or a non-immunoglobulin based antigen binding domain genetically linked to a marine luciferase. Malibu-Glo reagent can be conveniently produced in mammalian cells as a secreted protein that retains the functional activity of both the antigen binding domain and the luciferase. Moreover, crude supernatant containing the secreted Malibu-Glo reagent can directly be used for detection of cell surface antigens obviating the laborious steps of protein purification and labeling. We further demonstrate the utility of Malibu-Glo assay for the selection of optimal single chain fragment variables (scFvs) with desired affinity characteristics for incorporation into CARs. In summary, Malibu-Glo assay is a fast, simple, sensitive, specific and economical assay for antigen detection with multiple applications in the fields of antibody engineering, antibody humanization and CAR-T cell therapy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Natarajan V,Gopalakrishnan R,Matta H,Choi S,Gong S,Jeronimo A,Keerthipati PS,Morales A,Venkatesh H,Chaudhary PMdoi
10.1038/s41598-020-59099-9subject
Has Abstractpub_date
2020-02-11 00:00:00pages
2318issue
1issn
2045-2322pii
10.1038/s41598-020-59099-9journal_volume
10pub_type
杂志文章abstract：:In the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in parental cells, in a tissue specific manner, as a key step of cancer progression...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62920-0
更新日期：2020-04-16 00:00:00
abstract：:Epilepsy is a chronic neurological disease with a variable therapeutic response. To design effective treatment strategies for epilepsy, it is important to understand treatment responses and predictive factors. However, limited data are available in Africa, including Ethiopia. The aim of this study was therefore to ass...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52574-y
更新日期：2019-11-07 00:00:00
abstract：:Mycobacterium tuberculosis, the etiological agent of the infectious disease tuberculosis, kills approximately 1.5 million people annually, while the spread of multidrug-resistant strains is of great global concern. Thus, continuous efforts to identify new antitubercular drugs as well as novel targets are crucial. Rece...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21614-4
更新日期：2018-02-16 00:00:00
abstract：:High quality cadmium-free Zn-In-S:Ag doped-nanocrystals (d-NCs) were synthesized via a simple one-step noninjection route using silver nitrate, indium acetate, zinc acetate, oleylamine, S powder and 1-dodecanethiol as starting materials in an organic phase. The size and optical properties can be effectively tailored b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24459
更新日期：2016-04-14 00:00:00
abstract：:In order to understand the physical properties of materials it is necessary to determine the 3D positions of all atoms. There has been significant progress towards this goal using electron tomography. However, this method requires a relatively high electron dose and often extended acquisition times which precludes the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10654-x
更新日期：2017-09-07 00:00:00
abstract：:Sea ice is an important component of the global climate system and a key indicator of climate change. A decreasing trend in Arctic sea-ice concentration is evident in recent years, whereas Antarctic sea-ice concentration exhibits a generally increasing trend. Various studies have investigated the underlying causes of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45804
更新日期：2017-04-05 00:00:00
abstract：:Mucociliary clearance is a biomechanical mechanism of airway protection. It consists of the active transport along the bronchial tree of the mucus, a fluid propelled by the coordinated beating of a myriad of cilia on the epithelial surface of the respiratory tract. The physics of mucus transport is poorly understood b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20882-4
更新日期：2018-02-05 00:00:00
abstract：:Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipos...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-017-14570-y
更新日期：2017-10-26 00:00:00
abstract：:The domestic dog (Canis familiaris) is a promising animal model. Yet, the canine neuroscience literature is predominantly comprised of studies wherein (semi-)invasive methods and intensive training are used to study awake dog behavior. Given prior findings with humans and/or dogs, our goal was to assess, in 16 family ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25546-x
更新日期：2018-05-08 00:00:00
abstract：:Foods and beverage aroma results from multicomponent mixtures of volatile compounds present in the food that interact with olfactory receptors and produce a perceptual response in the brain. However, the perceptual interactions that occur when complex odor mixtures are combined are not well understood. Here we used Ga...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44064-y
更新日期：2019-05-21 00:00:00
abstract：:The Mongol invasion of Eastern Europe, and especially its sudden withdrawal from Hungary in 1242 CE, has generated much speculation and an array of controversial theories. None of them, however, considered multifaceted environmental drivers and the coupled analysis of historical reports and natural archives. Here we i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25606
更新日期：2016-05-26 00:00:00
abstract：:Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09532-3
更新日期：2017-08-22 00:00:00
abstract：:Src homology 2 domain-containing protein tyrosine phosphatase 2 (SHP-2) is a cytoplasmic tyrosine phosphatase that is highly expressed in hematopoietic cells and in the CNS and exerts opposite effects on signal transduction by exerting a neuroprotective or proapoptotic effect. Several mutations of SHP-2 have been foun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06401-x
更新日期：2017-07-20 00:00:00
abstract：:To compare the performance of eight score systems (MELD, uMELD, MELD-Na. iMELD, UKELD, MELD-AS, CTP, and mCTP) in predicting the post-transplant mortality, we analyzed the data of 6,014 adult cirrhotic patients who underwent liver transplantation between January 2003 and December 2010 from the China Liver Transplant R...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42253
更新日期：2017-02-13 00:00:00
abstract：:Inference of the biological roles of lncRNAs in breast cancer development remains a challenge. Here, we analyzed RNA-seq data in tumor and normal breast tissue samples from 18 breast cancer patients and 18 healthy controls and constructed a functional lncRNA-mRNA co-expression network. We revealed two distinctive co-e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32731
更新日期：2016-09-06 00:00:00
abstract：:Although aversive memory has been mainly addressed by analysing the changes occurring in average populations, the study of neuronal mechanisms of outliers allows understanding the involvement of individual differences in fear conditioning and extinction. We recently developed an innovative experimental model of indivi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21205
更新日期：2016-02-15 00:00:00
abstract：:The negatively-charged nitrogen vacancy (NV(-)) center in diamond is of great interest for quantum information processing and quantum key distribution applications due to its highly desirable long coherence times at room temperature. One of the challenges for their use in these applications involves the requirement to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11179
更新日期：2015-06-25 00:00:00
abstract：:A novel type of sub-lattice of the Jahn-Teller (JT) centers was arranged in Ti-doped barium hexaferrite BaFe12O19. In the un-doped crystal all iron ions, sitting in five different crystallographic positions, are Fe3+ in the high-spin configuration (S = 5/2) and have a non-degenerate ground state. We show that the elec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63915-7
更新日期：2020-04-27 00:00:00
abstract：:An investigation was carried out to assess the effect of the P content on the microstructure and mechanical properties of ZCuPb20Sn5 alloy. Alloys of various compositions, (0.05, 0.1, 0.2, 0.3, 0.5% wt.% P) were melted in a melting furnace under 1200 °C and cast into metal mould, the hardness, strength and elongation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49441-1
更新日期：2019-09-16 00:00:00
abstract：:Xenobiotic resistance is commonly found in species with short generation times such as bacteria, annual plants, and insects. Nevertheless, the fundamental evolutionary principles that govern the spread of resistance alleles hold true for species with longer generation times. One such example could occur with sea lampr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54260-5
更新日期：2019-12-03 00:00:00
abstract：:One of the potential antibiofilm strategies is to use lytic phages and phage-derived polysaccharide depolymerases. The idea is to uncover bacteria embedded in the biofilm matrix making them accessible and vulnerable to antibacterials and the immune system. Here we present the antibiofilm efficiency of lytic phage KP34...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77198-5
更新日期：2020-11-23 00:00:00
abstract：:With land privatization and fencing of thousands of hectares of communal grazing areas, East Africa is struggling with one of the most radical cultural and environmental changes in its history. The 668,500-hectare Greater Mara is of crucial importance for the great migrations of large mammals and for Maasai pastoralis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41450
更新日期：2017-01-25 00:00:00
abstract：:In recent years, the topic of noise in the sea and its effects on marine mammals has attracted considerable attention from both the scientific community and the general public. Since marine mammals rely heavily on acoustics as a primary means of communicating, navigating, and foraging in the ocean, any change in their...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00437
更新日期：2012-01-01 00:00:00
abstract：:Dietary information is lacking in most of small to mid-sized carnivores due to their elusive predatory behaviour and versatile feeding habits. The leopard cat (LPC; Prionailurus bengalensis) and the Asiatic golden cat (AGC; Catopuma temminckii) are two important yet increasingly endangered carnivore species in the tem...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41909
更新日期：2017-02-14 00:00:00
abstract：:Scattering of H2 on CO is of great importance in astrophysics and also is a benchmark system for comparing theory to experiment. We present here a new 6-dimensional potential energy surface for the ground electronic state of H2-CO with an estimated uncertainty of about 0.6 cm(-1) in the global minimum region, several ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28449
更新日期：2016-06-23 00:00:00
abstract：:With the reduction of oil and gas reserves and the increase of mining difficulty in Northern China, the carbonate rocks in Southern North China Basin are becoming a significant exploration target for carbonate reservoirs. However, the development characteristics, formation stages, formation environments and mechanisms...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80878-x
更新日期：2021-01-12 00:00:00
abstract：:Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44317
更新日期：2017-03-20 00:00:00
abstract：:Recognition of the potential for vegetated coastal ecosystems to store and sequester carbon has led to their increasing inclusion into global carbon budgets and carbon offset schemes. However, kelp forests have been overlooked in evaluations of this 'blue carbon', which have been limited to tidal marshes, mangrove for...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69258-7
更新日期：2020-07-23 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-34062-x
更新日期：2018-10-29 00:00:00
abstract：:Postsynaptic density protein-95 (PSD-95) is a major regulator in the maturation of excitatory synapses by interacting and trafficking N-methyl-D-aspartic acid receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isox-azoleproprionic acid receptors (AMPAR) to the postsynaptic membrane. PSD-95 disruption has recently been...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45971-w
更新日期：2019-07-01 00:00:00
