Abstract:
:Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.
journal_name
Sci Datajournal_title
Scientific dataauthors
Arakawa K,Yoshida Y,Tomita Mdoi
10.1038/sdata.2016.63subject
Has Abstractpub_date
2016-08-16 00:00:00pages
160063issn
2052-4463pii
sdata201663journal_volume
3pub_type
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