Genome sequencing of a single tardigrade Hypsibius dujardini individual.

Abstract:

:Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.

journal_name

Sci Data

journal_title

Scientific data

authors

Arakawa K,Yoshida Y,Tomita M

doi

10.1038/sdata.2016.63

subject

Has Abstract

pub_date

2016-08-16 00:00:00

pages

160063

issn

2052-4463

pii

sdata201663

journal_volume

3

pub_type

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