Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing.

Abstract:

:We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.

journal_name

Sci Data

journal_title

Scientific data

authors

Gorel A,Motomura K,Fukuzawa H,Doak RB,Grünbein ML,Hilpert M,Inoue I,Kloos M,Nass Kovács G,Nango E,Nass K,Roome CM,Shoeman RL,Tanaka R,Tono K,Foucar L,Joti Y,Yabashi M,Iwata S,Ueda K,Barends TRM,Schlichting I

doi

10.1038/sdata.2017.188

subject

Has Abstract

pub_date

2017-12-12 00:00:00

pages

170188

issn

2052-4463

pii

sdata2017188

journal_volume

4

pub_type

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