Abstract：
:Microalgae are versatile organisms capable of converting CO2, H2O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9 method rather than plasmids that encode Cas9 and guide RNAs. Outcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutively producing zeaxanthin and showing improved photosynthetic productivity.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Baek K,Kim DH,Jeong J,Sim SJ,Melis A,Kim JS,Jin E,Bae Sdoi
10.1038/srep30620subject
Has Abstractpub_date
2016-07-28 00:00:00pages
30620issn
2045-2322pii
srep30620journal_volume
6pub_type
杂志文章abstract：:The Fenton-like activity of nanoceria has attracted intensive attention for wastewater treatment in recent years. During the Fenton-like reaction, the adsorption of organic pollutants on catalyst surface plays a key role in their degradation. In this work, the adsorption-degradation of methylene blue (MB) and Congo re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36794-2
更新日期：2019-03-21 00:00:00
abstract：:The mammalian cochlea has historically resisted attempts at high-resolution, non-invasive imaging due to its small size, complex three-dimensional structure, and embedded location within the temporal bone. As a result, little is known about the relationship between an individual's cochlear pathology and hearing functi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33288
更新日期：2016-09-16 00:00:00
abstract：:Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30787-x
更新日期：2018-08-24 00:00:00
abstract：:Mal11 catalyzes proton-coupled maltose transport across the plasma membrane of Saccharomyces cerevisiae. We used structure-based design of mutants and a kinetic analysis of maltose transport to determine the energy coupling mechanism of transport. We find that wildtype Mal11 is extremely well coupled and allows yeast ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14438-1
更新日期：2017-10-30 00:00:00
abstract：:Outer membrane vesicles (OMVs) have proven to be highly immunogenic and induced an immune response against bacterial infection in human clinics and animal models. We sought to investigate whether engineered OMVs can be a feasible antigen-delivery platform for efficiently inducing specific antibody responses. In this s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37242
更新日期：2016-11-16 00:00:00
abstract：:The work describes the development of a flexible, hydrogel embedded pH-sensor that can be integrated in inexpensive wearable and non-invasive devices at epidermal level for electrochemical quantification of H+ ions in sweat. Such a device can be useful for swift, real time diagnosis and for monitoring specific conditi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45399-2
更新日期：2019-06-20 00:00:00
abstract：:Our previous studies have shown that sulbactam can play a neuroprotection role in hippocampal neurons by upregulating the expression and function of glial glutamate transporter-1 (GLT-1) during ischemic insult. Here, using rat global cerebral ischemia model, we studied in vivo the role of p38 mitogen-activated protein...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77583-0
更新日期：2020-11-26 00:00:00
abstract：:cAMP is one of the most important second messengers in biological processes. Cellular dynamics of cAMP have been investigated using a series of fluorescent indicators; however, their sensitivity was sub-optimal for detecting cAMP dynamics at a low concentration range, due to a low ligand affinity and/or poor dynamic r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20251-1
更新日期：2018-01-30 00:00:00
abstract：:Quantum walk is a key operation in quantum computing, simulation, communication and information. Here, we report for the first time the demonstration of quantum walks and localized quantum walks in a new type of optical fibers having a ring of cores constructed with both periodic and quasiperiodic Fibonacci sequences,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64065-6
更新日期：2020-04-28 00:00:00
abstract：:Dichapetalum cymosum produces the toxic fluorinated metabolite, fluoroacetate, presumably as a defence mechanism. Given the rarity of fluorinated metabolites in nature, the biosynthetic origin and function of fluoroacetate have been of particular interest. However, the mechanism for fluorination in D. cymosum was neve...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77598-7
更新日期：2020-11-25 00:00:00
abstract：:Elymus nutans is an herbaceous plant that can be used to restore degraded alpine and subalpine ecosystems. Here, we evaluated how sowing density affects soil reinforcement and slope stabilization properties of vegetation-concrete structures. To investigate the optimal sowing density of E. nutans in vegetation-concrete...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77407-1
更新日期：2020-11-24 00:00:00
abstract：:Drug addiction remains a prevalent and fatal disease worldwide that carries significant social and economic impacts. Recent reports suggest illicit pregabalin (Lyrica) use may be increasing among youth, however the addictive potential of pregabalin has not been well established. Drug seeking behavior and chronic drug ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51556-4
更新日期：2019-10-22 00:00:00
abstract：:We describe the realization and characterization of a compact, autonomous fiber laser system that produces the optical frequencies required for laser cooling, trapping, manipulation, and detection of 87Rb atoms - a typical atomic species for emerging quantum technologies. This device, a customized laser system from th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59971-8
更新日期：2020-02-24 00:00:00
abstract：:Epidemiological studies support a protective role of lycopene against stroke occurrence or mortality, but the results have been conflicting. We conducted a meta-analysis to assess the relationship between dietary or circulating lycopene and stroke risk (including stroke occurrence or mortality). Relevant papers were c...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep05031
更新日期：2014-05-22 00:00:00
abstract：:In this paper, we propose a real time, ultrahigh accuracy and full-field-of-view (RUF) algorithm for full field of view (FOV) imaging system. The proposed algorithm combines rough matching and precise matching method to stitch multiple images with the whole FOV in short time and high imaging quality. In order to verif...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69353-9
更新日期：2020-07-24 00:00:00
abstract：:Multiexcitonic transitions and emission of several photons per excitation comprise a very attractive feature of semiconductor quantum dots for optoelectronics applications. However, these higher-order radiative processes are usually quenched in colloidal quantum dots by Auger and other nonradiative decay channels. To ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42307
更新日期：2017-02-14 00:00:00
abstract：:We aimed to establish the collective influence of four lifestyle practices (physical activity, diet, smoking and alcohol consumption) on the prevalence and incidence of AMD. At baseline, 2428 participants aged 49+ with complete lifestyle and AMD data were examined, and of these, 1903 participants were re-examined 15 y...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04697-3
更新日期：2017-06-28 00:00:00
abstract：:Organic-inorganic hybrid perovskite materials have recently been identified as a promising light absorber for solar cells. In the efficient solar cells, the perovskite active layer has generally been fabricated by either vapor deposition or two-step sequential deposition process. Herein, electrochemically deposited Pb...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15889
更新日期：2015-10-29 00:00:00
abstract：:In this paper we present a contactless transient carrier spectroscopy and imaging technique for traps in silicon. At each pixel, we fit the transient decay of the trap emission which allows us to obtain both the trap time constant and trap concentration. Here we show that this technique allows for high-resolution imag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49804-8
更新日期：2019-10-03 00:00:00
abstract：:Kohlschutter-Tönz syndrome (KTS) is a rare genetic disorder with neurological dysfunctions including seizure and intellectual impairment. Mutations at the Rogdi locus have been linked to development of KTS, yet the underlying mechanisms remain elusive. Here we demonstrate that a Drosophila homolog of Rogdi acts as a n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11941-3
更新日期：2017-09-12 00:00:00
abstract：:Delivering liquid through the void spaces in porous metals is a daunting challenge for a variety of emerging interface technologies ranging from battery electrodes to evaporation surfaces. Hydraulic transport characteristics of well-ordered porous media are governed by the pore distribution, porosity, and morphology. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10791-3
更新日期：2017-09-05 00:00:00
abstract：:Estimating regional nutrient criteria for streams and rivers is a key step toward protecting river water quality and restoring the health of aquatic ecosystems. Using a multivariable statistical analysis technique, nutrients were identified as the main factor influencing the degradation of the benthic macroinvertebrat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22128-9
更新日期：2018-02-28 00:00:00
abstract：:A new simple method is proposed to extract the ambipolar diffusion length for two-dimensional (2D) electronic transport in thin film structures using a scanning photoluminescence microscopy (SPLM) setup. No spatially-resolved photoluminescence detection methods are required. By measuring the excitation-position-depend...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62093-w
更新日期：2020-03-23 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-61223-8
更新日期：2020-03-03 00:00:00
abstract：:Organ transplantation remains the most effective treatment for patients with late stage organ failure. Transgenic pigs provide an alternative organ donor source to the limited availability of human organs. However, cellular rejection still remains to be the obstacle for xenotransplantation. Superior to other methods, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04322-3
更新日期：2017-06-21 00:00:00
abstract：:Oral microbiome dysbiosis has been associated with various local and systemic human diseases such as dental caries, periodontal disease, obesity, and cardiovascular disease. Bacterial composition may be affected by age, oral health, diet, and geography, although information about the natural variation found in the gen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59016-0
更新日期：2020-02-07 00:00:00
abstract：:Fluorite-structured oxides find widespread use for applications spanning nuclear energy and waste containment, energy conversion, and sensing. In such applications the host tetravalent cation is often partially substituted by trivalent cations, with an associated formation of charge-compensating oxygen vacancies. The ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38772
更新日期：2016-12-12 00:00:00
abstract：:The interplay between the quasi 1-dimensional CuO-chains and the 2-dimensional CuO2 planes of YBa(2)Cu(3)O(6+x) (YBCO) has been in focus for a long time. Although the CuO-chains are known to be important as charge reservoirs that enable superconductivity for a range of oxygen doping levels in YBCO, the understanding o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07017
更新日期：2014-11-12 00:00:00
abstract：:Increased risk of comorbidities has been reported in Rheumatic and Musculoskeletal Diseases (RMD). We aimed to evaluate the prevalence and pattern of comorbidities in RMD patients nationwide, to identify multimorbidity clusters and to evaluate the gap between recommendations and real screening. Cross-sectional, multic...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-020-64732-8
更新日期：2020-05-06 00:00:00
abstract：:The development of 2D nanomaterial coatings across metal surfaces is a challenge due to the mismatch between the metal microstructure and the nanoscale materials. The naturally occurring thin oxidative layer present across all metal surfaces, may lead to low adherence and connectivity. In this paper, graphene/titania/...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26447-9
更新日期：2018-05-25 00:00:00
