The putative phospholipase Lip2 counteracts oxidative damage and influences the virulence of Ustilago maydis.

Abstract:

:Ustilago maydis is an obligate biotrophic fungal pathogen which causes common smut disease of corn. To proliferate in host tissue, U. maydis must gain access to nutrients and overcome plant defence responses, such as the production of reactive oxygen species. The elucidation of the mechanisms by which U. maydis meets these challenges is critical for the development of strategies to combat smut disease. In this study, we focused on the contributions of phospholipases (PLs) to the pathogenesis of corn smut disease. We identified 11 genes encoding putative PLs and characterized the transcript levels for these genes in the fungus grown in culture and during infection of corn tissue. To assess the contributions of specific PLs, we focused on two genes, lip1 and lip2, which encode putative phospholipase A2 (PLA2 ) enzymes with similarity to platelet-activating factor acetylhydrolases. PLA2 enzymes are known to counteract oxidative damage to lipids in other organisms. Consistent with a role in the mitigation of oxidative damage, lip2 mutants were sensitive to oxidative stress provoked by hydrogen peroxide and by increased production of reactive oxygen species caused by inhibitors of mitochondrial functions. Importantly, mutants defective in lip2, but not lip1, were attenuated for virulence in corn seedlings. Finally, a comparative analysis of fatty acid and cardiolipin profiles in the wild-type strain and a lip2 mutant revealed differences consistent with a protective role for Lip2 in maintaining lipid homeostasis and mitochondrial health during proliferation in the hostile host environment.

journal_name

Mol Plant Pathol

authors

Lambie SC,Kretschmer M,Croll D,Haslam TM,Kunst L,Klose J,Kronstad JW

doi

10.1111/mpp.12391

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

210-221

issue

2

eissn

1464-6722

issn

1364-3703

journal_volume

18

pub_type

杂志文章
  • Genetic comparison of Ug99 with selected South African races of Puccinia graminis f.sp. tritici.

    abstract::Using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) marker analyses, the genetic structure of selected South African wheat stem rust races was compared with Ug99. SSR analysis divided the population into two distinct groups with 24.5% similarity between them. A local race, UVPgt55 (Nor...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2008.00525.x

    authors: Visser B,Herselman L,Pretorius ZA

    更新日期:2009-03-01 00:00:00

  • Penicillium expansum: biology, omics, and management tools for a global postharvest pathogen causing blue mould of pome fruit.

    abstract::Blue mould, caused primarily by Penicillium expansum, is a major threat to the global pome fruit industry, causing multimillion-dollar losses annually. The blue mould fungus negatively affects fruit quality, thereby reducing fresh fruit consumption, and significantly contributes to food loss. P. expansum also produces...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12990

    authors: Luciano-Rosario D,Keller NP,Jurick WM 2nd

    更新日期:2020-11-01 00:00:00

  • The expression, function and regulation of mitochondrial alternative oxidase under biotic stresses.

    abstract::To survive, plants possess elaborate defence mechanisms to protect themselves against virus or pathogen invasion. Recent studies have suggested that plant mitochondria may play an important role in host defence responses to biotic stresses. In contrast with animal mitochondria, plant mitochondria possess a unique resp...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1364-3703.2010.00615.x

    authors: Hanqing F,Kun S,Mingquan L,Hongyu L,Xin L,Yan L,Yifeng W

    更新日期:2010-05-01 00:00:00

  • A dsbA mutant of Pseudomonas syringae exhibits reduced virulence and partial impairment of type III secretion.

    abstract::Abstract To identify virulence genes of P. syringae pv. tomato strain DC3000 we screened for mutants with reduced virulence on its plant hosts, Arabidopsis thaliana and tomato. We isolated a Tn5-insertion mutant that exhibited reduced virulence on both hosts. Further characterization showed that this mutant carried a ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2000.00016.x

    authors: Kloek AP,Brooks DM,Kunkel BN

    更新日期:2000-03-01 00:00:00

  • A novel Meloidogyne enterolobii effector MeTCTP promotes parasitism by suppressing programmed cell death in host plants.

    abstract::Meloidogyne enterolobii is one of the most important plant-parasitic nematodes that can overcome the Mi-1 resistance gene and damage many economically important crops. Translationally controlled tumour protein (TCTP) is a multifunctional protein that exists in various eukaryotes and plays an important role in parasiti...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12374

    authors: Zhuo K,Chen J,Lin B,Wang J,Sun F,Hu L,Liao J

    更新日期:2017-01-01 00:00:00

  • A curious case of resistance to a new encounter pathogen: myrtle rust in Australia.

    abstract::Resistance genes (R genes) in plants mediate a highly specific response to microbial pathogens, often culminating in localized cell death. Such resistance is generally pathogen race specific and believed to be the result of evolutionary selection pressure. Where a host and pathogen do not share an evolutionary history...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12331

    authors: Tobias PA,Guest DI,Külheim C,Hsieh JF,Park RF

    更新日期:2016-06-01 00:00:00

  • Application of high-throughput genome sequencing to intrapathovar variation in Pseudomonas syringae.

    abstract::One reason for the success of Pseudomonas syringae as a model pathogen has been the availability of three complete genome sequences since 2005. Now, at the beginning of 2011, more than 25 strains of P. syringae have been sequenced and many more will soon be released. To date, published analyses of P. syringae have bee...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1364-3703.2011.00713.x

    authors: Studholme DJ

    更新日期:2011-10-01 00:00:00

  • Molecular mechanisms that limit the costs of NLR-mediated resistance in plants.

    abstract::Crop diseases cause significant yield losses, and the use of resistant cultivars can effectively mitigate these losses and control many plant diseases. Most plant resistance (R) genes encode immune receptors composed of nucleotide-binding and leucine-rich repeat (NLR) domains. These proteins mediate the specific recog...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12723

    authors: Richard MMS,Gratias A,Meyers BC,Geffroy V

    更新日期:2018-11-01 00:00:00

  • Differential roles of glucosinolates and camalexin at different stages of Agrobacterium-mediated transformation.

    abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease in a wide range of plants via a unique interkingdom DNA transfer from bacterial cells into the plant genome. Agrobacterium tumefaciens is capable of transferring its T-DNA into different plant parts at different developmental stages for transient and ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12672

    authors: Shih PY,Chou SJ,Müller C,Halkier BA,Deeken R,Lai EM

    更新日期:2018-03-02 00:00:00

  • Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation.

    abstract::MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus-infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illu...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12717

    authors: Wang S,Cui W,Wu X,Yuan Q,Zhao J,Zheng H,Lu Y,Peng J,Lin L,Chen J,Yan F

    更新日期:2018-11-01 00:00:00

  • De novo generation of Lettuce infectious yellows virus defective RNAs in protoplasts.

    abstract::Summary Lettuce infectious yellows virus (LIYV)-infected plants contain a heterogeneous population of defective RNAs (D RNAs) derived from LIYV genomic RNA 2. To partly address how LIYV D RNAs are generated, in vitro synthesized transcripts corresponding to the LIYV genomic RNAs 1 and 2 were inoculated to protoplasts,...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2002.00125.x

    authors: Rubio L,Tian T,Yeh HH,Livieratos Y,Falk BW

    更新日期:2002-09-01 00:00:00

  • A new point mutation in the iron-sulfur subunit of succinate dehydrogenase confers resistance to boscalid in Sclerotinia sclerotiorum.

    abstract::Research has established that mutations in highly conserved amino acids of the succinate dehydrogenase (SDH) complex in various fungi confer SDH inhibitor (SDHI) resistance. For Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic fungus with a broad host range and a worldwide distribution, boscalid resistance has ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12222

    authors: Wang Y,Duan Y,Wang J,Zhou M

    更新日期:2015-09-01 00:00:00

  • Mycosphaerella graminicola: latent infection, crop devastation and genomics.

    abstract:UNLABELLED:summary Mycosphaerella graminicola is an important pathogen of wheat, causing septoria leaf blotch disease. This review summarizes the current knowledge on disease development and control of the fungus, and discusses how molecular tools and genomics are being employed to uncover the genetic basis of pathogen...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1464-6722.2002.00100.x

    authors: Palmer CL,Skinner W

    更新日期:2002-03-01 00:00:00

  • Sources of target specificity associated with the recovery against Pea seed-borne mosaic virus infection mediated by RNA silencing in pea.

    abstract::SUMMARY Transgenic peas containing a copy of the Pea seed-borne mosaic virus (PSbMV) isolate DPD1 NIb sequence develop a 'recovery' phenotype when challenged with either the homologous (DPD1) or a heterologous (NY) PSbMV isolate. However, the specificity of the subsequent resistance differs with respect to the initiat...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2004.00204.x

    authors: Van Den Boogaart T,Maule AJ,Davies JW,Lomonossoff GP

    更新日期:2004-01-01 00:00:00

  • Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) genes expressed during infection of cotton (Gossypium hirsutum)dagger.

    abstract::SUMMARY We sought to identify Fusarium oxysporum f. sp. vasinfectum (Fov) genes that may be associated with pathogenicity. Initially we utilized microarray and Q-PCR technology to identify Fov genes expressed in root and hypocotyl tissues during a compatible infection of cotton. We identified 218 fungal clones represe...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2006.00327.x

    authors: McFadden HG,Wilson IW,Chapple RM,Dowd C

    更新日期:2006-03-01 00:00:00

  • Strawberry crinkle virus, a Cytorhabdovirus needing more attention from virologists.

    abstract::Summary Taxonomic relationship: A member of nonsegmented, negative-sense, single-stranded RNA viruses of the genus Cytorhabdovirus (type member: Lettuce necrotic yellows virus), family Rhabdoviridae, order Mononegavirales. Members of the family Rhabdoviridae can infect vertebrates, invertebrates and plants. Physical p...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2000.00041.x

    authors: Posthuma KI,Adams AN,Hong Y

    更新日期:2000-11-01 00:00:00

  • Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus.

    abstract::SUMMARY The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was develope...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00434.x

    authors: François C,Castagnone C,Boonham N,Tomlinson J,Lawson R,Hockland S,Quill J,Vieira P,Mota M,Castagnone-Sereno P

    更新日期:2007-11-01 00:00:00

  • A nonribosomal peptide synthetase mediates siderophore production and virulence in the citrus fungal pathogen Alternaria alternata.

    abstract::Alternaria species produce and excrete dimethyl coprogen siderophores to acquire iron. The Alternaria alternata gene AaNPS6, encoding a polypeptide analogous to fungal nonribosomal peptide synthetases, was found to be required for the production of siderophores and virulence on citrus. Siderophores purified from cultu...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12021

    authors: Chen LH,Lin CH,Chung KR

    更新日期:2013-06-01 00:00:00

  • Piriformospora indica-a mutualistic basidiomycete with an exceptionally large plant host range.

    abstract::Piriformospora indica is a basidiomycete of the order Sebacinales, representing a model for the study of mutualistic symbiosis and, beyond that, the plant immune system. The fungus colonizes the roots of a wide range of vascular plants, increasing their growth, seed yield and adaptation to abiotic and biotic stresses....

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1364-3703.2011.00764.x

    authors: Qiang X,Weiss M,Kogel KH,Schäfer P

    更新日期:2012-06-01 00:00:00

  • Signalling requirements for Erwinia amylovora-induced disease resistance, callose deposition and cell growth in the non-host Arabidopsis thaliana.

    abstract::Erwinia amylovora is the causal agent of the fire blight disease in some plants of the Rosaceae family. The non-host plant Arabidopsis serves as a powerful system for the dissection of mechanisms of resistance to E. amylovora. Although not yet known to mount gene-for-gene resistance to E. amylovora, we found that Arab...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12588

    authors: Hamdoun S,Gao M,Gill M,Kwon A,Norelli JL,Lu H

    更新日期:2018-05-01 00:00:00

  • NEMATIC: a simple and versatile tool for the in silico analysis of plant-nematode interactions.

    abstract::Novel approaches for the control of agriculturally damaging nematodes are sorely needed. Endoparasitic nematodes complete their life cycle within the root vascular cylinder, inducing specialized feeding cells: giant cells for root-knot nematodes and syncytia for cyst nematodes. Both nematodes hijack parts of the trans...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12114

    authors: Cabrera J,Bustos R,Favery B,Fenoll C,Escobar C

    更新日期:2014-08-01 00:00:00

  • The PCR amplification and characterization of entire Pseudomonas syringae hrp/hrc clusters.

    abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2004.00212.x

    authors: Gropp SJ,Guttman DS

    更新日期:2004-03-01 00:00:00

  • Differential activation of ABI3 and LEA genes upon plant parasitic nematode infection.

    abstract::SUMMARY Promoter activity of ABI3 and of three LEA genes was monitored in Arabidopsis transgenics infected with Heterodera schachtii and Meloidogyne incognita. ABI3::GUS expression was induced (in four different promoter deletion constructs) during early infection stages with H. schachtii. Similar GUS expression patte...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2005.00283.x

    authors: DE Meutter J,Robertson L,Parcy F,Mena M,Fenoll C,Gheysen G

    更新日期:2005-05-01 00:00:00

  • Tsw gene-based resistance is triggered by a functional RNA silencing suppressor protein of the Tomato spotted wilt virus.

    abstract::As a result of contradictory reports, the avirulence (Avr) determinant that triggers Tsw gene-based resistance in Capsicum annuum against the Tomato spotted wilt virus (TSWV) is still unresolved. Here, the N and NSs genes of resistance-inducing (RI) and resistance-breaking (RB) isolates were cloned and transiently exp...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12016

    authors: de Ronde D,Butterbach P,Lohuis D,Hedil M,van Lent JW,Kormelink R

    更新日期:2013-05-01 00:00:00

  • Reduced susceptibility to Fusarium head blight in Brachypodium distachyon through priming with the Fusarium mycotoxin deoxynivalenol.

    abstract::The fungal cereal pathogen Fusarium graminearum produces deoxynivalenol (DON) during infection. The mycotoxin DON is associated with Fusarium head blight (FHB), a disease that can cause vast grain losses. Whilst investigating the suitability of Brachypodium distachyon as a model for spreading resistance to F. graminea...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12203

    authors: Blümke A,Sode B,Ellinger D,Voigt CA

    更新日期:2015-06-01 00:00:00

  • Metabolic adaptation of Phytophthora infestans during growth on leaves, tubers and artificial media.

    abstract::Efficient nutrient acquisition is critical to the fitness of plant pathogens. To address how the late blight agent Phytophthora infestans adapts to nutrients offered by its hosts, genes in glycolytic, gluconeogenic and amino acid pathways were mined from its genome and their expression in different plant tissues and a...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2009.00570.x

    authors: Judelson HS,Tani S,Narayan RD

    更新日期:2009-11-01 00:00:00

  • The fungal-specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae.

    abstract::Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transc...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12367

    authors: Luo X,Mao H,Wei Y,Cai J,Xie C,Sui A,Yang X,Dong J

    更新日期:2016-12-01 00:00:00

  • The role of plant defence proteins in fungal pathogenesis.

    abstract::SUMMARY It is becoming increasingly evident that a plant-pathogen interaction may be compared to an open warfare, whose major weapons are proteins synthesized by both organisms. These weapons were gradually developed in what must have been a multimillion-year evolutionary game of ping-pong. The outcome of each battle ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00419.x

    authors: Ferreira RB,Monteiro S,Freitas R,Santos CN,Chen Z,Batista LM,Duarte J,Borges A,Teixeira AR

    更新日期:2007-09-01 00:00:00

  • Small RNA inhibits infection by downy mildew pathogen Hyaloperonospora arabidopsidis.

    abstract::Gene silencing exists in eukaryotic organisms as a conserved regulation of the gene expression mechanism. In general, small RNAs (sRNAs) are produced within the eukaryotic cells and incorporated into an RNA-induced silencing complex (RISC) within cells. However, exogenous sRNAs, once delivered into cells, can also sil...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12863

    authors: Bilir Ö,Telli O,Norman C,Budak H,Hong Y,Tör M

    更新日期:2019-11-01 00:00:00

  • A novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencing.

    abstract::The single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Fou...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12366

    authors: Untiveros M,Olspert A,Artola K,Firth AE,Kreuze JF,Valkonen JP

    更新日期:2016-09-01 00:00:00