Abstract:
:SUMMARY The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was developed to detect this organism. Specific probe and primers were designed based on the sequence of the MspI satellite DNA family previously characterized in the genome of the nematode. The method proved to be specific in tests with target DNA from PWN isolates from worldwide origin. From a practical point of view, detection limit was 1 pg of target DNA or one individual nematode. In addition, PWN genomic DNA or single individuals were positively detected in mixed samples in which B. xylophilius was associated with the closely related non-pathogenic species B. mucronatus, up to the limit of 0.01% or 1% of the mixture, respectively. The real-time PCR assay was also used in conjunction with a simple DNA extraction method to detect PWN directly in artificially infested wood samples. These results demonstrate the potential of this assay to provide rapid, accurate and sensitive molecular identification of the PWN in relation to pest risk assessment in the field and quarantine regulation.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
François C,Castagnone C,Boonham N,Tomlinson J,Lawson R,Hockland S,Quill J,Vieira P,Mota M,Castagnone-Sereno Pdoi
10.1111/j.1364-3703.2007.00434.xsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
803-9issue
6eissn
1464-6722issn
1364-3703pii
MPP434journal_volume
8pub_type
杂志文章abstract::SUMMARY Pathogenic fungi have successfully attacked a wide range of hosts, which has forced them into ambient-adaptation. pH is one of the major ambient traits affecting the activity of pathogenicity factors secreted by the pathogen, hence, a pH sensing-response system was developed to enable the pathogen to tailor it...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00196.x
更新日期:2003-11-01 00:00:00
abstract::Sugar metabolism and sugar signalling are not only critical for plant growth and development, but are also important for stress responses. However, how sugar homeostasis is involved in plant defence against pathogen attack in the model crop rice remains largely unknown. In this study, we observed that the grains of gi...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12078
更新日期:2014-02-01 00:00:00
abstract::SUMMARY A new BglII satellite DNA has been isolated, cloned and sequenced from the coffee root-knot nematode, Meloidogyne exigua (Nematoda: Tylenchida). It is represented as tandemly repeated sequences with a monomeric unit of 277 bp. The monomers are present at approximately 17 900 copies per haploid genome, and repr...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2002.00134.x
更新日期:2002-11-01 00:00:00
abstract::SUMMARY Here, we consider the barley powdery mildew fungus, Blumeria graminis (DC Speer) f.sp. hordei (Marchal), and review recent research which has added to our understanding of the biology and molecular biology which underpins the asexual life cycle of this potentially devastating pathogen. We focus on the early st...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00303.x
更新日期:2005-09-01 00:00:00
abstract::Efficient nutrient acquisition is critical to the fitness of plant pathogens. To address how the late blight agent Phytophthora infestans adapts to nutrients offered by its hosts, genes in glycolytic, gluconeogenic and amino acid pathways were mined from its genome and their expression in different plant tissues and a...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00570.x
更新日期:2009-11-01 00:00:00
abstract::Many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. However, to our knowledge, no such list exists. The aim of this review was to survey all plant virologists with an association with Molecular Plant Pathology and ask them to nominate which...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2011.00752.x
更新日期:2011-12-01 00:00:00
abstract::Phytohormones, such as salicylic acid (SA), ethylene (ET) and jasmonic acid (JA), play key roles in plant defence following pathogen attack. The involvement of these hormones in susceptibility following Fusarium oxysporum (Fo) infection has mostly been studied in Arabidopsis thaliana. However, Fo causes vascular wilt ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12559
更新日期:2017-09-01 00:00:00
abstract::A high-throughput RNA-mediated gene silencing system was developed for Cochliobolus sativus (anamorph: Bipolaris sorokiniana), the causal agent of spot blotch, common root rot and black point in barley and wheat. The green fluorescent protein gene (GFP) and the proteinaceous host-selective toxin gene (ToxA) were first...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00666.x
更新日期:2011-04-01 00:00:00
abstract::Summary A root knot nematode resistance gene in Nicotiana tabacum, Rk, providing resistance to the nematode parasite Meloidogyne incognita is tightly linked to, or is a pleiotropic gene with a veinal necrosis systemic hypersensitive response to infection by Potato virus Y (PVY) M(s)N(r). The single PVY M(s)N(r) open r...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2002.00106.x
更新日期:2002-05-01 00:00:00
abstract::Pattern recognition receptors (PRRs) can trigger plant immunity through the recognition of pathogen-associated molecular patterns. In this study, we report that a malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, from Haynaldia villosa putatively acts as a PRR to positively regulate powdery mildew...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12729
更新日期:2018-12-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a serious disease of rice. Xoo secretes a repertoire of cell wall-degrading enzymes, including cellulases, xylanases and pectinases, to degrade various polysaccharide components of the rice cell wall. A secreted Xoo cellulase, CbsA, is not only a key virulen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12620
更新日期:2018-06-01 00:00:00
abstract::Frosty pod rot (FPR) of Theobroma cacao (cacao) is caused by the hemibiotrophic fungus Moniliophthora roreri. Cacao clones tolerant to FPR are being planted throughout Central America. To determine whether M. roreri shows a differential molecular response during successful infections of tolerant clones, we collected f...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12134
更新日期:2014-09-01 00:00:00
abstract::The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector-dominant host sensitivity gene interactions. The three best-characterized effector-sensitivity gene systems are SnToxA-Tsn1, SnTox1-Snn1 ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12511
更新日期:2017-04-01 00:00:00
abstract::Membrane trafficking is vital to plant development and adaptation to the environment. It is suggested that post-Golgi vesicles and multivesicular bodies are essential for plant defence against directly penetrating fungal parasites at the cell wall. However, the actual plant proteins involved in membrane transport for ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00846.x
更新日期:2013-04-01 00:00:00
abstract::Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweet potato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including sweet potato feathery m...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00480.x
更新日期:2008-09-01 00:00:00
abstract::Successful host-pathogen interactions require the presence, maintenance and expression of gene cassettes called 'pathogenicity islands' (PAIs) and 'metabolic islands' (MAIs) in the respective pathogen. The products of these genes confer on the pathogen the means to recognize their host(s) and to efficiently evade host...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2008.00518.x
更新日期:2009-01-01 00:00:00
abstract::In plants, RNA silencing is a surveillance mechanism against invading viruses. It involves the production of virus-derived small interfering RNAs (vsiRNAs), which guide the RNA-induced silencing complex (RISC) to inactivate viruses. vsiRNAs may also promote the silencing of host mRNAs in a sequence-specific manner. In...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00828.x
更新日期:2013-01-01 00:00:00
abstract::SUMMARY Recent studies have shown that resistance in several dicotyledonous plants to viruses in the genus Potyvirus is controlled by recessive alleles of the plant translation initiation factor eIF4E or eIF(iso)4E genes. Here we provide evidence that the barley rym4 gene locus, controlling immunity to viruses in the ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00294.x
更新日期:2005-07-01 00:00:00
abstract::A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contrib...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00820.x
更新日期:2012-12-01 00:00:00
abstract::Protein kinases have been implicated in the regulation of many processes that guide pathogen development throughout the course of infection. A survey of the Sclerotinia sclerotiorum genome for genes encoding proteins containing the highly conserved eukaryotic protein kinase (ePK) domain, the largest protein kinase sup...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12321
更新日期:2016-05-01 00:00:00
abstract::The fungal cereal pathogen Fusarium graminearum produces deoxynivalenol (DON) during infection. The mycotoxin DON is associated with Fusarium head blight (FHB), a disease that can cause vast grain losses. Whilst investigating the suitability of Brachypodium distachyon as a model for spreading resistance to F. graminea...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12203
更新日期:2015-06-01 00:00:00
abstract::Flower malformation represented by phyllody is a common symptom of phytoplasma infection induced by a novel family of phytoplasma effectors called phyllogens. Despite the accumulation of functional and structural phyllogen information, the molecular mechanisms of phyllody have not yet been integrated with their evolut...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12981
更新日期:2020-10-01 00:00:00
abstract::SUMMARY Fungal hydrophobins are small hydrophobic proteins containing eight cysteine residues at conserved positions which have the ability to form amphipathic polymers. We have characterized a gene from the phytopathogenic ascomycete Claviceps purpurea, cpph1, which encodes a modular-type hydrophobin. It consists of ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00138.x
更新日期:2003-01-01 00:00:00
abstract::Gene silencing exists in eukaryotic organisms as a conserved regulation of the gene expression mechanism. In general, small RNAs (sRNAs) are produced within the eukaryotic cells and incorporated into an RNA-induced silencing complex (RISC) within cells. However, exogenous sRNAs, once delivered into cells, can also sil...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12863
更新日期:2019-11-01 00:00:00
abstract::Abstract To test whether carbohydrates may play a signalling function during plant pathogenesis, we investigated the interaction between tobacco and potato virus Y (PVY(N)). Four days after PVY(N) infection, leaves started to accumulate soluble sugars and leaf photosynthesis decreased. The accumulation of soluble suga...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00007.x
更新日期:2000-01-01 00:00:00
abstract::SUMMARY Promoter activity of ABI3 and of three LEA genes was monitored in Arabidopsis transgenics infected with Heterodera schachtii and Meloidogyne incognita. ABI3::GUS expression was induced (in four different promoter deletion constructs) during early infection stages with H. schachtii. Similar GUS expression patte...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00283.x
更新日期:2005-05-01 00:00:00
abstract::The vascular pathogen Xanthomonas oryzae pv. oryzae (Xoo) and nonvascular pathogen Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight (BB) and bacterial leaf streak (BLS) diseases of rice, respectively. We have previously identified the avirulence gene avrXa27 from Xoo PXO99(A), which specifically induces t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00509.x
更新日期:2009-01-01 00:00:00
abstract::Biotrophic fungal pathogens must evade or suppress plant defence responses to establish a compatible interaction in living host tissue. In addition, metabolic changes during disease reflect both the impact of nutrient acquisition by the fungus to support proliferation and the integration of metabolism with the plant d...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12485
更新日期:2017-12-01 00:00:00
abstract::NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1; also known as NIM1) is a master regulator of systemic acquired resistance (SAR). SAR is induced by salicylic acid (SA), leading to the expression of PATHOGENESIS-RELATED (PR) genes. Current evidence suggests that NPR1 is part of a transcription complex tethered to ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00653.x
更新日期:2011-01-01 00:00:00
abstract::Recently, Tomato spotted wilt virus (TSWV) nonstructural protein NSs has been identified unambiguously as an avirulence (Avr) determinant for Tomato spotted wilt (Tsw)-based resistance. The observation that NSs from two natural resistance-breaking isolates had lost RNA silencing suppressor (RSS) activity and Avr sugge...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12082
更新日期:2014-02-01 00:00:00