Chloroplast-associated metabolic functions influence the susceptibility of maize to Ustilago maydis.

Abstract:

:Biotrophic fungal pathogens must evade or suppress plant defence responses to establish a compatible interaction in living host tissue. In addition, metabolic changes during disease reflect both the impact of nutrient acquisition by the fungus to support proliferation and the integration of metabolism with the plant defence response. In this study, we used transcriptome analyses to predict that the chloroplast and associated functions are important for symptom formation by the biotrophic fungus Ustilago maydis on maize. We tested our prediction by examining the impact on disease of a genetic defect (whirly1) in chloroplast function. In addition, we examined whether disease was influenced by inhibition of glutamine synthetase by glufosinate (impacting amino acid biosynthesis) or inhibition of 3-phosphoshikimate 1-carboxyvinyltransferase by glyphosate (influencing secondary metabolism). All of these perturbations increased the severity of disease, thus suggesting a contribution to resistance. Overall, these findings provide a framework for understanding the components of host metabolism that benefit the plant versus the pathogen during a biotrophic interaction. They also reinforce the emerging importance of the chloroplast as a mediator of plant defence.

journal_name

Mol Plant Pathol

authors

Kretschmer M,Croll D,Kronstad JW

doi

10.1111/mpp.12485

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

1210-1221

issue

9

eissn

1464-6722

issn

1364-3703

journal_volume

18

pub_type

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