Distribution and pathway for phloem-dependent movement of Melon necrotic spot virus in melon plants.

Abstract:

:The translocation of Melon necrotic spot virus (MNSV) within tissues of inoculated and systemically infected Cucumis melo L. 'Galia' was studied by tissue-printing and in situ hybridization techniques. The results were compatible with the phloem vascular components being used to spread MNSV systemically by the same assimilate transport route that runs from source to sink organs. Virus RNAs were shown to move from the inoculated cotyledon toward the hypocotyl and root system via the external phloem, whereas the upward spread through the stem to the young tissues took place via the internal phloem. Virus infection was absent from non-inoculated source tissues as well as from both shoot and root apical meristems, but active sink tissues such as the young leaves and root system were highly infected. Finally, our results suggest that the MNSV invasion of roots is due to virus replication although a destination-selective process is probably necessary to explain the high levels of virus accumulation in roots. This efficient invasion of the root system is discussed in terms of natural transmission of MNSV by the soil-borne fungal vector.

journal_name

Mol Plant Pathol

authors

Gosalvez-Bernal B,Genoves A,Navarro JA,Pallas V,Sanchez-Pina MA

doi

10.1111/j.1364-3703.2008.00474.x

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

447-61

issue

4

eissn

1464-6722

issn

1364-3703

pii

MPP474

journal_volume

9

pub_type

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