Constitutive heterologous expression of avrXa27 in rice containing the R gene Xa27 confers enhanced resistance to compatible Xanthomonas oryzae strains.

Abstract:

:The vascular pathogen Xanthomonas oryzae pv. oryzae (Xoo) and nonvascular pathogen Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight (BB) and bacterial leaf streak (BLS) diseases of rice, respectively. We have previously identified the avirulence gene avrXa27 from Xoo PXO99(A), which specifically induces the expression of the rice resistance gene Xa27, ultimately leading to resistance against BB disease in rice. In this study, we have generated a transgenic rice line (L24) that expresses avrXa27 constitutively under the control of the PR1 promoter, and have examined its role in the host-pathogen interaction. L24 is not more susceptible to BB, indicating that avrXa27 does not contribute to virulence. AvrXa27 retains avirulence activity in L24 and, after crossing with a line containing Xa27, progeny display phenotypic changes including inhibition of tillering, delay in flowering, stiff leaves, early leaf senescence and activation of pathogenesis-related (PR) genes. On challenge with a variety of compatible strains of Xoo and Xoc strain L8, lines with both avrXa27 and Xa27 also show enhanced resistance to bacterial infection. The induction of Xa27 and subsequent inhibition of Xoc growth in Xa27 plants are observed on inoculation with Xoc L8 harbouring avrXa27. Our results indicate that the heterologous expression of avrXa27 in rice containing Xa27 triggers R gene-specific resistance and, at the same time, confers enhanced resistance to compatible strains of Xoo and Xoc. The expression of AvrXa27 and related proteins in plants has the potential to generate broad resistance in plants.

journal_name

Mol Plant Pathol

authors

Tian D,Yin Z

doi

10.1111/j.1364-3703.2008.00509.x

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

29-39

issue

1

eissn

1464-6722

issn

1364-3703

pii

MPP509

journal_volume

10

pub_type

杂志文章
  • Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 (GIF1) plays a role in pre-existing and induced defence in rice.

    abstract::Sugar metabolism and sugar signalling are not only critical for plant growth and development, but are also important for stress responses. However, how sugar homeostasis is involved in plant defence against pathogen attack in the model crop rice remains largely unknown. In this study, we observed that the grains of gi...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12078

    authors: Sun L,Yang DL,Kong Y,Chen Y,Li XZ,Zeng LJ,Li Q,Wang ET,He ZH

    更新日期:2014-02-01 00:00:00

  • The synthetic cationic lipid diC14 activates a sector of the Arabidopsis defence network requiring endogenous signalling components.

    abstract::Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12252

    authors: Cambiagno DA,Lonez C,Ruysschaert JM,Alvarez ME

    更新日期:2015-12-01 00:00:00

  • Evidence that the recessive bymovirus resistance locus rym4 in barley corresponds to the eukaryotic translation initiation factor 4E gene.

    abstract::SUMMARY Recent studies have shown that resistance in several dicotyledonous plants to viruses in the genus Potyvirus is controlled by recessive alleles of the plant translation initiation factor eIF4E or eIF(iso)4E genes. Here we provide evidence that the barley rym4 gene locus, controlling immunity to viruses in the ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2005.00294.x

    authors: Kanyuka K,Druka A,Caldwell DG,Tymon A,McCallum N,Waugh R,Adams MJ

    更新日期:2005-07-01 00:00:00

  • Loss of CMD2-mediated resistance to cassava mosaic disease in plants regenerated through somatic embryogenesis.

    abstract::Cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) are the two most important viral diseases affecting cassava production in Africa. Three sources of resistance are employed to combat CMD: polygenic recessive resistance, termed CMD1, the dominant monogenic type, named CMD2, and the recently character...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12353

    authors: Beyene G,Chauhan RD,Wagaba H,Moll T,Alicai T,Miano D,Carrington JC,Taylor NJ

    更新日期:2016-09-01 00:00:00

  • Comparative proteomic analysis of Xanthomonas citri ssp. citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction.

    abstract::Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm-enriched fraction was performed for XAC cells grown in pathogenicit...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12507

    authors: Artier J,da Silva Zandonadi F,de Souza Carvalho FM,Pauletti BA,Leme AFP,Carnielli CM,Selistre-de-Araujo HS,Bertolini MC,Ferro JA,Belasque Júnior J,de Oliveira JCF,Novo-Mansur MTM

    更新日期:2018-01-01 00:00:00

  • Possible involvement of maize Rop1 in the defence responses of plants to viral infection.

    abstract::The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was construct...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2011.00782.x

    authors: Cao Y,Shi Y,Li Y,Cheng Y,Zhou T,Fan Z

    更新日期:2012-09-01 00:00:00

  • Melon RNA interference (RNAi) lines silenced for Cm-eIF4E show broad virus resistance.

    abstract::Efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. Recentl...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2012.00785.x

    authors: Rodríguez-Hernández AM,Gosalvez B,Sempere RN,Burgos L,Aranda MA,Truniger V

    更新日期:2012-09-01 00:00:00

  • Extensive chromosomal rearrangements and rapid evolution of novel effector superfamilies contribute to host adaptation and speciation in the basal ascomycetous fungi.

    abstract::The basal ascomycetes in genus Taphrina have strict host specificity and coevolution with their host plants, making them appealing models for studying the genomic basis of ecological divergence and host adaption. We therefore performed genome sequencing and comparative genomics of different Taphrina species with disti...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12899

    authors: Wang Q,Sun M,Zhang Y,Song Z,Zhang S,Zhang Q,Xu JR,Liu H

    更新日期:2020-03-01 00:00:00

  • Roles of plant hormones in the regulation of host-virus interactions.

    abstract::Hormones are tuners of plant responses to biotic and abiotic stresses. They are involved in various complicated networks, through which they modulate responses to different stimuli. Four hormones primarily regulate plant defence to pathogens: salicylic acid (SA), jasmonic acid (JA), ethylene (Et) and abscisic acid (AB...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12204

    authors: Alazem M,Lin NS

    更新日期:2015-06-01 00:00:00

  • Comparison of transcript profiles in different life stages of the nematode Globodera pallida under different host potato genotypes.

    abstract::The potato cyst nematodes (PCNs) Globodera pallida and Globodera rostochiensis are important parasites of potato. PCNs undergo complex biotrophic interactions with their hosts that involve gene expression changes in both the nematode and the host plant. The aim of this study was to determine key genes that are differe...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2012.00821.x

    authors: Palomares-Rius JE,Hedley PE,Cock PJ,Morris JA,Jones JT,Vovlas N,Blok V

    更新日期:2012-12-01 00:00:00

  • Fungal attack and host defence pathways unveiled in near-avirulent interactions of Penicillium expansum creA mutants on apples.

    abstract::Amongst the universal diseases affecting apples, blue mould caused by Penicillium expansum is a major concern, resulting in yield and quality losses as a result of the production of the mycotoxin patulin. Despite the characterization of the patulin biosynthetic gene cluster at both the molecular and chemical levels, t...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12734

    authors: Tannous J,Kumar D,Sela N,Sionov E,Prusky D,Keller NP

    更新日期:2018-12-01 00:00:00

  • Expression of Arabidopsis pathogenesis-related genes during nematode infection.

    abstract::The expression pattern of pathogenesis-related genes PR-1 to PR-5 was examined in the roots and leaves of Arabidopsis thaliana plants on infection with beet-cyst (Heterodera schachtii) and root-knot (Meloidogyne incognita) nematodes. During H. schachtii parasitism of Arabidopsis, the expression of PR-1, PR-2 and PR-5,...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2010.00675.x

    authors: Hamamouch N,Li C,Seo PJ,Park CM,Davis EL

    更新日期:2011-05-01 00:00:00

  • The broad bacterial blight resistance of rice line CBB23 is triggered by a novel transcription activator-like (TAL) effector of Xanthomonas oryzae pv. oryzae.

    abstract::Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is not only a disease devastating rice production worldwide, but also an ideal model system for the study of the interaction between plants and their bacterial pathogens. The rice near-isogenic line (NIL) CBB23, derived from a cross between a wild r...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12092

    authors: Wang CL,Qin TF,Yu HM,Zhang XP,Che JY,Gao Y,Zheng CK,Yang B,Zhao KJ

    更新日期:2014-05-01 00:00:00

  • Related mobile pathogenicity chromosomes in Fusarium oxysporum determine host range on cucurbits.

    abstract::Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) causes severe root rot and wilt in several cucurbit species, including cucumber, melon, and watermelon. Previously, a pathogenicity chromosome, chrRC , was identified in Forc. Strains that were previously nonpathogenic could infect multiple cucurbit species after ob...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12927

    authors: Li J,Fokkens L,van Dam P,Rep M

    更新日期:2020-06-01 00:00:00

  • The signalling mucin Msb2 regulates surface sensing and host penetration via BMP1 MAP kinase signalling in Botrytis cinerea.

    abstract::Botrytis cinerea is a necrotrophic fungus that infects a wide range of fruit, vegetable and flower crops. Penetration of the host cuticle occurs via infection structures that are formed in response to appropriate plant surface signals. The differentiation of these structures requires a highly conserved mitogen-activat...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12234

    authors: Leroch M,Mueller N,Hinsenkamp I,Hahn M

    更新日期:2015-10-01 00:00:00

  • Eukaryotic translation initiation factor 4E-mediated recessive resistance to plant viruses and its utility in crop improvement.

    abstract::The use of genetic resistance is considered to be the most effective and sustainable approach to the control of plant pathogens. Although most of the known natural resistance genes are monogenic dominant R genes that are predominant against fungi and bacteria, more and more recessive resistance genes against viruses h...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1364-3703.2012.00791.x

    authors: Wang A,Krishnaswamy S

    更新日期:2012-09-01 00:00:00

  • Fluorescent reporter analysis revealed the timing and localization of AVR-Pia expression, an avirulence effector of Magnaporthe oryzae.

    abstract::In order to facilitate infection, the rice blast pathogen Magnaporthe oryzae secretes an abundance of proteins, including avirulence effectors, to diminish its host's defences. Avirulence effectors are recognized by host resistance proteins and trigger the host's hypersensitive response, which is a rapid and effective...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12468

    authors: Sornkom W,Miki S,Takeuchi S,Abe A,Asano K,Sone T

    更新日期:2017-10-01 00:00:00

  • The TNL gene Rdr1 confers broad-spectrum resistance to Diplocarpon rosae.

    abstract::Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, probably together with commercial cultivars. Here, we present data indicating that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12589

    authors: Menz I,Straube J,Linde M,Debener T

    更新日期:2018-05-01 00:00:00

  • Differential roles of glucosinolates and camalexin at different stages of Agrobacterium-mediated transformation.

    abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease in a wide range of plants via a unique interkingdom DNA transfer from bacterial cells into the plant genome. Agrobacterium tumefaciens is capable of transferring its T-DNA into different plant parts at different developmental stages for transient and ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12672

    authors: Shih PY,Chou SJ,Müller C,Halkier BA,Deeken R,Lai EM

    更新日期:2018-03-02 00:00:00

  • Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation.

    abstract::Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12809

    authors: Li Y,Héloir MC,Zhang X,Geissler M,Trouvelot S,Jacquens L,Henkel M,Su X,Fang X,Wang Q,Adrian M

    更新日期:2019-08-01 00:00:00

  • The conserved oligomeric Golgi complex is involved in penetration resistance of barley to the barley powdery mildew fungus.

    abstract::Membrane trafficking is vital to plant development and adaptation to the environment. It is suggested that post-Golgi vesicles and multivesicular bodies are essential for plant defence against directly penetrating fungal parasites at the cell wall. However, the actual plant proteins involved in membrane transport for ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2012.00846.x

    authors: Ostertag M,Stammler J,Douchkov D,Eichmann R,Hückelhoven R

    更新日期:2013-04-01 00:00:00

  • Plant pathogenic Verticillium species: how many of them are there?

    abstract::SUMMARY Two of the currently widely accepted species in the section Nigrescentia of the genus Verticillium are major plant pathogens inducing wilt diseases in a wide range of mainly dicotyledonous hosts. Three species closely related to these two are less important wilt pathogens and soil saprophytes. A sixth species,...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2003.00172.x

    authors: Barbara DJ,Clewes E

    更新日期:2003-07-01 00:00:00

  • Transcriptomic changes in Nicotiana benthamiana plants inoculated with the wild-type or an attenuated mutant of Tobacco vein banding mosaic virus.

    abstract::Tobacco vein banding mosaic virus (TVBMV) is a potyvirus which mainly infects solanaceous crops. The helper component proteinase (HCpro) of a potyvirus is an RNA silencing suppressor protein and determines the severity of disease symptoms caused by different potyviruses, including TVBMV. It has been shown that substit...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12471

    authors: Geng C,Wang HY,Liu J,Yan ZY,Tian YP,Yuan XF,Gao R,Li XD

    更新日期:2017-10-01 00:00:00

  • Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet.

    abstract::SUMMARY We previously reported that cell wall protein fractions (CWPs) of the biocontrol agent Pythium oligandrum have elicitor properties in sugar beet and wheat. Here we have examined the effect of treatment with the D-type of CWP, a fraction that contains two major forms (POD-1 and POD-2), on the induction of defen...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2006.00340.x

    authors: Takenaka S,Nakamura Y,Kono T,Sekiguchi H,Masunaka A,Takahashi H

    更新日期:2006-09-01 00:00:00

  • The putative phospholipase Lip2 counteracts oxidative damage and influences the virulence of Ustilago maydis.

    abstract::Ustilago maydis is an obligate biotrophic fungal pathogen which causes common smut disease of corn. To proliferate in host tissue, U. maydis must gain access to nutrients and overcome plant defence responses, such as the production of reactive oxygen species. The elucidation of the mechanisms by which U. maydis meets ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12391

    authors: Lambie SC,Kretschmer M,Croll D,Haslam TM,Kunst L,Klose J,Kronstad JW

    更新日期:2017-02-01 00:00:00

  • Differential gene expression by Moniliophthora roreri while overcoming cacao tolerance in the field.

    abstract::Frosty pod rot (FPR) of Theobroma cacao (cacao) is caused by the hemibiotrophic fungus Moniliophthora roreri. Cacao clones tolerant to FPR are being planted throughout Central America. To determine whether M. roreri shows a differential molecular response during successful infections of tolerant clones, we collected f...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12134

    authors: Bailey BA,Melnick RL,Strem MD,Crozier J,Shao J,Sicher R,Phillips-Mora W,Ali SS,Zhang D,Meinhardt L

    更新日期:2014-09-01 00:00:00

  • One gene-one name: the AvrLmJ1 avirulence gene of Leptosphaeria maculans is AvrLm5.

    abstract::Leptosphaeria maculans, the causal agent of blackleg disease, interacts with Brassica napus (oilseed rape, canola) and other Brassica hosts in a gene-for-gene manner. The avirulence gene AvrLmJ1 has been cloned previously and shown to interact with an unidentified Brassica juncea resistance gene. In this study, we sho...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12574

    authors: Plissonneau C,Rouxel T,Chèvre AM,Van De Wouw AP,Balesdent MH

    更新日期:2018-04-01 00:00:00

  • Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola LS102 requires two unlinked loci.

    abstract::Plant-pathogenic oomycetes secrete effector proteins to suppress host immune responses. Resistance proteins may recognize effectors and activate immunity, which is often associated with a hypersensitive response (HR). Transient expression of effectors in plant germplasm and screening for HR has proven to be a powerful...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12751

    authors: Pelgrom AJE,Eikelhof J,Elberse J,Meisrimler CN,Raedts R,Klein J,Van den Ackerveken G

    更新日期:2019-02-01 00:00:00

  • Silencing PsKPP4, a MAP kinase kinase kinase gene, reduces pathogenicity of the stripe rust fungus.

    abstract::Many obligately parasitic pathogens absorb nutrients from host plants via specialized infection structures, called haustoria and infection hyphae, to further colonization and growth in the host plant. In the wheat (Triticum aestivum) stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), the mitogen-activated ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12731

    authors: Zhu X,Guo J,He F,Zhang Y,Tan C,Yang Q,Huang C,Kang Z,Guo J

    更新日期:2018-12-01 00:00:00

  • Mycosphaerella graminicola: latent infection, crop devastation and genomics.

    abstract:UNLABELLED:summary Mycosphaerella graminicola is an important pathogen of wheat, causing septoria leaf blotch disease. This review summarizes the current knowledge on disease development and control of the fungus, and discusses how molecular tools and genomics are being employed to uncover the genetic basis of pathogen...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1464-6722.2002.00100.x

    authors: Palmer CL,Skinner W

    更新日期:2002-03-01 00:00:00