Abstract:
:Many obligately parasitic pathogens absorb nutrients from host plants via specialized infection structures, called haustoria and infection hyphae, to further colonization and growth in the host plant. In the wheat (Triticum aestivum) stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), the mitogen-activated protein kinase kinase (MAPKK) PsFUZ7 is involved in the regulation of haustorium formation and invasive growth. Here, we functionally characterized PsKPP4 of Pst, which is homologous to the yeast MAPKKK STE11. Similar to the silencing of PsFUZ7, the knockdown of PsKPP4 was detected in the vegetative hyphae and haustoria, resulting in the reduced pathogenicity of Pst. Pst urediniospores treated with the STE11 MAPKKK activation inhibitor produced deformed germ tubes. In addition, overexpression of PsKPP4 in fission yeast resulted in the production of fusiform cells and increased tolerance of yeast cells to oxidative stress. The transformation of PsKPP4 into the mst11 mutant of Magnaporthe oryzae partially restored mst11 function. The PsKPP4 protein contains a sterile alpha motif (SAM), Ras association (RA) and kinase domains, similar to its homologues in other fungi. Yeast two-hybrid assays revealed that the SAM domain is essential for the interaction between PsKPP4 and PsUBC2, a homologue of Ustilago maydis UBC2, known to interact with KPP4, which is associated with the regulation of the Fus3 cascade. Host-induced gene silencing of PsUBC2 reduced the pathogenicity of Pst slightly, indicating that PsUBC2 also plays a minor role in the regulation of the infection pathway of Pst. These observations indicate that PsKPP4, interacting with PsUBC2, may play an important role in the regulation of infection-related morphogenesis in Pst.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Zhu X,Guo J,He F,Zhang Y,Tan C,Yang Q,Huang C,Kang Z,Guo Jdoi
10.1111/mpp.12731subject
Has Abstractpub_date
2018-12-01 00:00:00pages
2590-2602issue
12eissn
1464-6722issn
1364-3703journal_volume
19pub_type
杂志文章abstract::The expression pattern of pathogenesis-related genes PR-1, PR-2 and PR-5, considered as markers for salicylic acid (SA)-dependent systemic acquired resistance (SAR), was examined in the roots and shoots of tomato plants pre-treated with SA and subsequently infected with root-knot nematodes (RKNs) (Meloidogyne incognit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12085
更新日期:2014-04-01 00:00:00
abstract::LuxR-type regulators regulate many bacterial processes and play important roles in bacterial motility and virulence. Acidovorax citrulli is a seedborne bacterial pathogen responsible for bacterial fruit blotch, which causes great losses in melon and watermelon worldwide. We identified a LuxR-type, nonquorum sensing-re...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12910
更新日期:2020-04-01 00:00:00
abstract::Pratylenchus penetrans is one of the most important species of root lesion nematodes (RLNs) because of its detrimental and economic impact in a wide range of crops. Similar to other plant-parasitic nematodes (PPNs), P. penetrans harbours a significant number of secreted proteins that play key roles during parasitism. ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12666
更新日期:2018-02-09 00:00:00
abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00212.x
更新日期:2004-03-01 00:00:00
abstract::Summary Taxonomic relationship: A member of nonsegmented, negative-sense, single-stranded RNA viruses of the genus Cytorhabdovirus (type member: Lettuce necrotic yellows virus), family Rhabdoviridae, order Mononegavirales. Members of the family Rhabdoviridae can infect vertebrates, invertebrates and plants. Physical p...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00041.x
更新日期:2000-11-01 00:00:00
abstract::summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated us...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00079.x
更新日期:2001-11-01 00:00:00
abstract::Homologous recombination is required for gene-targeted procedures such as gene disruption and gene replacement. Ku80 is part of the non-homologous end-joining DNA repair mechanism in many organisms. We identified and disrupted the Ku80 homologue in Sclerotinia sclerotiorum and generated heterokaryon mutants enriched w...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00483.x
更新日期:2008-09-01 00:00:00
abstract::The life cycle of the plant pathogen Erwinia amylovora comprises periods inside and outside the host in which it faces oxidative stress caused by hydrogen peroxide (H2 O2 ) and other compounds. The sources of this stress are plant defences, other microorganisms and/or exposure to starvation or other environmental chal...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12577
更新日期:2018-04-01 00:00:00
abstract::SUMMARY Brachiaria is a pan-tropical genus of grasses with about 100 species. The fungus Acremonium implicatum can develop an endophytic association that is mutually beneficial with Brachiaria species. We developed a polymerase chain reaction (PCR)-based method by first amplifying DNA from A. implicatum isolates using...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00157.x
更新日期:2003-03-01 00:00:00
abstract::Blue mould, caused primarily by Penicillium expansum, is a major threat to the global pome fruit industry, causing multimillion-dollar losses annually. The blue mould fungus negatively affects fruit quality, thereby reducing fresh fruit consumption, and significantly contributes to food loss. P. expansum also produces...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12990
更新日期:2020-11-01 00:00:00
abstract::The effector gene Avr1b-1 of Phytophthora sojae determines the efficacy of the resistance gene Rps1b in soybean. The sequences of the Avr1b-1 locus in 34 Chinese isolates of P. sojae were obtained and analysed by polymerase chain reaction (PCR) and inverse PCR. Four different alleles and a complete deletion mutation o...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00733.x
更新日期:2012-02-01 00:00:00
abstract::Abstract In prokaryotes, a diverse set of physiological processes is regulated by transcription factors which belong to the well-conserved, enhancer-binding protein (EBP) family. These regulatory proteins function together with the alternate sigma factor (sigma(54)). Structurally, the EBPs are characterized by a centr...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00028.x
更新日期:2000-07-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a serious disease of rice. Xoo secretes a repertoire of cell wall-degrading enzymes, including cellulases, xylanases and pectinases, to degrade various polysaccharide components of the rice cell wall. A secreted Xoo cellulase, CbsA, is not only a key virulen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12620
更新日期:2018-06-01 00:00:00
abstract::The analysis of the interaction between Arabidopsis thaliana and adapted (PcBMM) and nonadapted (Pc2127) isolates of the necrotrophic fungus Plectosphaerella cucumerina has contributed to the identification of molecular mechanisms controlling plant resistance to necrotrophs. To characterize the pathogenicity bases of ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00826.x
更新日期:2013-01-01 00:00:00
abstract::RXLR effectors encoded by Phytophthora species play a central role in pathogen-plant interactions. An understanding of the biological functions of RXLR effectors is conducive to the illumination of the pathogenic mechanisms and the development of disease control strategies. However, the virulence function of Phytophth...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12760
更新日期:2019-03-01 00:00:00
abstract::Amongst the universal diseases affecting apples, blue mould caused by Penicillium expansum is a major concern, resulting in yield and quality losses as a result of the production of the mycotoxin patulin. Despite the characterization of the patulin biosynthetic gene cluster at both the molecular and chemical levels, t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12734
更新日期:2018-12-01 00:00:00
abstract::Burkholderia gladioli is a causal agent of bacterial panicle blight and sheath/grain browning in rice in many countries. Many strains produce the yellow pigment toxoflavin, which is highly toxic to plants, fungi, animals and microorganisms. Although there have been several studies on the toxoflavin biosynthesis system...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12262
更新日期:2016-01-01 00:00:00
abstract::Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm-enriched fraction was performed for XAC cells grown in pathogenicit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12507
更新日期:2018-01-01 00:00:00
abstract::The expression pattern of pathogenesis-related genes PR-1 to PR-5 was examined in the roots and leaves of Arabidopsis thaliana plants on infection with beet-cyst (Heterodera schachtii) and root-knot (Meloidogyne incognita) nematodes. During H. schachtii parasitism of Arabidopsis, the expression of PR-1, PR-2 and PR-5,...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00675.x
更新日期:2011-05-01 00:00:00
abstract::Histone acetylation has been established as a principal epigenetic regulatory mechanism in eukaryotes. Sas3, a histone acetyltransferase belonging to the largest family of acetyltransferase, MYST, is the catalytic subunit of a conserved histone acetyltransferase complex. To date, the functions of Sas3 and its ortholog...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12856
更新日期:2019-11-01 00:00:00
abstract::SUMMARY The hxc1 mutant was identified by screening an EMS (ethylmethane sulphonate) mutagenized population of Arabidopsis Col-0 plants for an altered hypersensitive response (HR), after spray inoculation with an HR-inducing isolate of Xanthomonas campestris pv. campestris (Xcc) (strain 147). The hxc1 mutant shows a s...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00245.x
更新日期:2004-09-01 00:00:00
abstract::SUMMARY The phytotoxin coronatine (COR) contributes to the virulence of Pseudomonas syringae pv. tomato (Pst) strain DC3000 on Arabidopsis thaliana and tomato. However, little is known regarding the role of COR in the virulence of DC3000 on cultivated Brassica spp. In this study, the role of COR and its precursors, co...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00372.x
更新日期:2007-01-01 00:00:00
abstract:UNLABELLED:summary Mycosphaerella graminicola is an important pathogen of wheat, causing septoria leaf blotch disease. This review summarizes the current knowledge on disease development and control of the fungus, and discusses how molecular tools and genomics are being employed to uncover the genetic basis of pathogen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2002.00100.x
更新日期:2002-03-01 00:00:00
abstract::Coffee (Coffea arabica L.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus Hemileia vastatrix. The transcriptome of H. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed dur...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00723.x
更新日期:2012-01-01 00:00:00
abstract::Leptosphaeria maculans, the causal agent of blackleg disease, interacts with Brassica napus (oilseed rape, canola) and other Brassica hosts in a gene-for-gene manner. The avirulence gene AvrLmJ1 has been cloned previously and shown to interact with an unidentified Brassica juncea resistance gene. In this study, we sho...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12574
更新日期:2018-04-01 00:00:00
abstract::Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is not only a disease devastating rice production worldwide, but also an ideal model system for the study of the interaction between plants and their bacterial pathogens. The rice near-isogenic line (NIL) CBB23, derived from a cross between a wild r...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12092
更新日期:2014-05-01 00:00:00
abstract::Plant-pathogenic oomycetes secrete effector proteins to suppress host immune responses. Resistance proteins may recognize effectors and activate immunity, which is often associated with a hypersensitive response (HR). Transient expression of effectors in plant germplasm and screening for HR has proven to be a powerful...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12751
更新日期:2019-02-01 00:00:00
abstract::SUMMARY Lipopolysaccharides (LPS) are indispensable cell surface components of Gram-negative bacteria and have diverse roles in plant-microbe interactions. Treatment of Nicotiana tabacum with the LPS of an endophytic strain of Burkholderia cepacia results in an enhanced defensive capacity in the tissue. In this study ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00234.x
更新日期:2004-07-01 00:00:00
abstract::Systemic acquired resistance (SAR) is a potent plant defence system that, in response to a first contact with a plant pathogen, prepares the whole plant for subsequent attacks, so that it becomes more resistant to the same and to other pathogens. BcSpl1, a cerato-platanin family protein abundantly secreted by Botrytis...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00842.x
更新日期:2013-02-01 00:00:00
abstract::Using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) marker analyses, the genetic structure of selected South African wheat stem rust races was compared with Ug99. SSR analysis divided the population into two distinct groups with 24.5% similarity between them. A local race, UVPgt55 (Nor...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00525.x
更新日期:2009-03-01 00:00:00