Identification of a lipopolysaccharide responsive erk-like MAP kinase in tobacco leaf tissue.

Abstract:

:SUMMARY Lipopolysaccharides (LPS) are indispensable cell surface components of Gram-negative bacteria and have diverse roles in plant-microbe interactions. Treatment of Nicotiana tabacum with the LPS of an endophytic strain of Burkholderia cepacia results in an enhanced defensive capacity in the tissue. In this study the rapid and transient phosphorylation of an extracellular signal-regulated (ERK)-like mitogen-activated protein (MAP) kinase in response to LPS from B. cepacia (LPS(B.cep.)) elicitation is reported. Based on in-gel kinase assays it was found that this 43-kDa LPS(B.cep.)-responsive kinase is optimally activated following 7 min elicitation with 100 microg/mL LPS. Its identity as an ERK MAPK was supported by tyrosine-phosphorylated association with induction, immunodetection with pTEpY-specific MAPK antibodies and inhibition of phosphorylation by U0126, an upstream MAPKK inhibitor. The kinase utilized myelin basic protein, but not casein or histone, as substrate. Ca(2+) did not modulate the phosphorylation, nor did wounding. To date, other MAP kinases have been shown to act either independently or upstream from reactive oxygen intermediates produced during the oxidative burst. It was found that hydrogen peroxide is either not generated in leaf tissue in response to LPS elicitation or, if generated, does not trigger the phosphorylation of the kinase. Physicochemical characterization of the ERK-like MAPK indicated a molecular mass of 43 kDa and a pI of 6.3; two-dimensional gel analysis indicated two charge isomers. This is the first demonstration of such an LPS-responsive MAP kinase phosphorylation in plants.

journal_name

Mol Plant Pathol

authors

Piater LA,Nürnberger T,Dubery IA

doi

10.1111/j.1364-3703.2004.00234.x

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

331-41

issue

4

eissn

1464-6722

issn

1364-3703

pii

MPP234

journal_volume

5

pub_type

杂志文章
  • The fungal-specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae.

    abstract::Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transc...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12367

    authors: Luo X,Mao H,Wei Y,Cai J,Xie C,Sui A,Yang X,Dong J

    更新日期:2016-12-01 00:00:00

  • The role of PilZ domain proteins in the virulence of Xanthomonas campestris pv. campestris.

    abstract::Cyclic di-GMP [(bis-(3'-5')-cyclic di-guanosine monophosphate)] is an almost ubiquitous second messenger in bacteria that is implicated in the regulation of a range of functions that include developmental transitions, aggregative behaviour, adhesion, biofilm formation and virulence. Comparatively little is known about...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2008.00495.x

    authors: McCarthy Y,Ryan RP,O'Donovan K,He YQ,Jiang BL,Feng JX,Tang JL,Dow JM

    更新日期:2008-11-01 00:00:00

  • Abscisic acid deficiency leads to rapid activation of tomato defence responses upon infection with Erwinia chrysanthemi.

    abstract::In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear to have a negative effect on disease resistance. Using the ABA-deficient sitiens tomato (Solanum lycopersicum) mutant and different application methods of exogenous ABA, we demonstrated the influence...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00437.x

    authors: Asselbergh B,Achuo AE,Höfte M,Van Gijsegem F

    更新日期:2008-01-01 00:00:00

  • Agrobacterium T-DNA-encoded protein Atu6002 interferes with the host auxin response.

    abstract::Several genes in the Agrobacterium tumefaciens transferred (T)-DNA encode proteins that are involved in developmental alterations, leading to the formation of tumours in infected plants. We investigated the role of the protein encoded by the Atu6002 gene, the function of which is completely unknown. Atu6002 expression...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12088

    authors: Lacroix B,Gizatullina DI,Babst BA,Gifford AN,Citovsky V

    更新日期:2014-04-01 00:00:00

  • Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis.

    abstract::SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expres...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00394.x

    authors: Kudla U,Milac AL,Qin L,Overmars H,Roze E,Holterman M,Petrescu AJ,Goverse A,Bakker J,Helder J,Smant G

    更新日期:2007-05-01 00:00:00

  • The Potato virus Y M(S)N(R) NIb-replicase is the elicitor of a veinal necrosis-hypersensitive response in root knot nematode resistant tobacco.

    abstract::Summary A root knot nematode resistance gene in Nicotiana tabacum, Rk, providing resistance to the nematode parasite Meloidogyne incognita is tightly linked to, or is a pleiotropic gene with a veinal necrosis systemic hypersensitive response to infection by Potato virus Y (PVY) M(s)N(r). The single PVY M(s)N(r) open r...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2002.00106.x

    authors: Fellers JP,Tremblay D,Handest MF,Lommel SA

    更新日期:2002-05-01 00:00:00

  • Plant pathogenic Verticillium species: how many of them are there?

    abstract::SUMMARY Two of the currently widely accepted species in the section Nigrescentia of the genus Verticillium are major plant pathogens inducing wilt diseases in a wide range of mainly dicotyledonous hosts. Three species closely related to these two are less important wilt pathogens and soil saprophytes. A sixth species,...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2003.00172.x

    authors: Barbara DJ,Clewes E

    更新日期:2003-07-01 00:00:00

  • Pectobacterium atrosepticum KDPG aldolase, Eda, participates in the Entner-Doudoroff pathway and independently inhibits expression of virulence determinants.

    abstract::Pectobacterium carotovorum has an incomplete Entner-Doudoroff (ED) pathway, including enzyme 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) but lacking phosphogluconate dehydratase (Edd), while P. atrosepticum (Pba) has a complete pathway. To understand the role of the ED pathway in Pectobacterium infection, mutants...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.13025

    authors: Wang H,Wang Y,Humphris S,Nie W,Zhang P,Wright F,Campbell E,Hu B,Fan J,Toth I

    更新日期:2021-02-01 00:00:00

  • The eggplant AG91-25 recognizes the Type III-secreted effector RipAX2 to trigger resistance to bacterial wilt (Ralstonia solanacearum species complex).

    abstract::To deploy durable plant resistance, we must understand its underlying molecular mechanisms. Type III effectors (T3Es) and their recognition play a central role in the interaction between bacterial pathogens and crops. We demonstrate that the Ralstonia solanacearum species complex (RSSC) T3E ripAX2 triggers specific re...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12724

    authors: Morel A,Guinard J,Lonjon F,Sujeeun L,Barberis P,Genin S,Vailleau F,Daunay MC,Dintinger J,Poussier S,Peeters N,Wicker E

    更新日期:2018-11-01 00:00:00

  • A novel Meloidogyne enterolobii effector MeTCTP promotes parasitism by suppressing programmed cell death in host plants.

    abstract::Meloidogyne enterolobii is one of the most important plant-parasitic nematodes that can overcome the Mi-1 resistance gene and damage many economically important crops. Translationally controlled tumour protein (TCTP) is a multifunctional protein that exists in various eukaryotes and plays an important role in parasiti...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12374

    authors: Zhuo K,Chen J,Lin B,Wang J,Sun F,Hu L,Liao J

    更新日期:2017-01-01 00:00:00

  • Identification of potato genes induced during colonization by Phytophthora infestans.

    abstract::Summary Suppression Subtractive Hybridization (SSH) was applied in a search for genes induced during the compatible interaction between Phytophthora infestans and potato. Using potato leaves that had been treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methylester (BTH) as the control tissue, a low redundanc...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2001.00059.x

    authors: Beyer K,Binder A,Boller T,Collinge M

    更新日期:2001-05-01 00:00:00

  • Mutual interplay between phytopathogenic powdery mildew fungi and other microorganisms.

    abstract::Powdery mildew is a common and widespread plant disease of considerable agronomic relevance. It is caused by obligate biotrophic fungal pathogens which, in most cases, epiphytically colonize aboveground plant tissues. The disease has been typically studied as a binary interaction of the fungal pathogen with its plant ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12771

    authors: Panstruga R,Kuhn H

    更新日期:2019-04-01 00:00:00

  • Monolignol biosynthesis is associated with resistance to Sclerotinia sclerotiorum in Camelina sativa.

    abstract::The ascomycete Sclerotinia sclerotiorum is a necrotrophic plant pathogen with an extremely broad host range. It causes stem rot in Camelina sativa, a crucifer with great potential as an alternative oilseed crop. Lignification is a common phenomenon in the expression of resistance against necrotrophs, but the molecular...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2012.00798.x

    authors: Eynck C,Séguin-Swartz G,Clarke WE,Parkin IA

    更新日期:2012-10-01 00:00:00

  • Differential regulation of toxoflavin production and its role in the enhanced virulence of Burkholderia gladioli.

    abstract::Burkholderia gladioli is a causal agent of bacterial panicle blight and sheath/grain browning in rice in many countries. Many strains produce the yellow pigment toxoflavin, which is highly toxic to plants, fungi, animals and microorganisms. Although there have been several studies on the toxoflavin biosynthesis system...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12262

    authors: Lee J,Park J,Kim S,Park I,Seo YS

    更新日期:2016-01-01 00:00:00

  • Survival factor 1 contributes to the oxidative stress response and is required for full virulence of Sclerotinia sclerotiorum.

    abstract::Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen that infects over 400 species of plants worldwide. Reactive oxygen species (ROS) modulations are critical for the pathogenic development of S. sclerotiorum. The fungus applies enzymatic and non-enzymatic antioxidants to cope with the oxidative stre...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12801

    authors: Yu Y,Du J,Wang Y,Zhang M,Huang Z,Cai J,Fang A,Yang Y,Qing L,Bi C,Cheng J

    更新日期:2019-07-01 00:00:00

  • Effective tolerance based on resource reallocation is a virus-specific defence in Arabidopsis thaliana.

    abstract::Plant viruses often harm their hosts, which have developed mechanisms to prevent or minimize the effects of virus infection. Resistance and tolerance are the two main plant defences to pathogens. Although resistance to plant viruses has been studied extensively, tolerance has received much less attention. Theory predi...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12629

    authors: Shukla A,Pagán I,García-Arenal F

    更新日期:2018-06-01 00:00:00

  • Roles of plant hormones in the regulation of host-virus interactions.

    abstract::Hormones are tuners of plant responses to biotic and abiotic stresses. They are involved in various complicated networks, through which they modulate responses to different stimuli. Four hormones primarily regulate plant defence to pathogens: salicylic acid (SA), jasmonic acid (JA), ethylene (Et) and abscisic acid (AB...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12204

    authors: Alazem M,Lin NS

    更新日期:2015-06-01 00:00:00

  • The eukaryotic protein kinase superfamily of the necrotrophic fungal plant pathogen, Sclerotinia sclerotiorum.

    abstract::Protein kinases have been implicated in the regulation of many processes that guide pathogen development throughout the course of infection. A survey of the Sclerotinia sclerotiorum genome for genes encoding proteins containing the highly conserved eukaryotic protein kinase (ePK) domain, the largest protein kinase sup...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12321

    authors: Hegedus DD,Gerbrandt K,Coutu C

    更新日期:2016-05-01 00:00:00

  • Multi-factor regulation of pectate lyase secretion by Colletotrichum gloeosporioides pathogenic on avocado fruits.

    abstract::Tissue alkalinization during Colletotrichum gloeosporioides attack enhances the expression of PELB, which encodes pectate lyase (PL), and PL secretion, which is considered essential for full virulence. We studied the regulation of PL secretion by manipulation of C. gloeosporioides PELB. PELB was down-regulated by knoc...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00462.x

    authors: Miyara I,Shafran H,Kramer Haimovich H,Rollins J,Sherman A,Prusky D

    更新日期:2008-05-01 00:00:00

  • Efficient gene replacement and direct hyphal transformation in Sclerotinia sclerotiorum.

    abstract::Homologous recombination is required for gene-targeted procedures such as gene disruption and gene replacement. Ku80 is part of the non-homologous end-joining DNA repair mechanism in many organisms. We identified and disrupted the Ku80 homologue in Sclerotinia sclerotiorum and generated heterokaryon mutants enriched w...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2008.00483.x

    authors: Levy M,Erental A,Yarden O

    更新日期:2008-09-01 00:00:00

  • The TNL gene Rdr1 confers broad-spectrum resistance to Diplocarpon rosae.

    abstract::Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, probably together with commercial cultivars. Here, we present data indicating that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12589

    authors: Menz I,Straube J,Linde M,Debener T

    更新日期:2018-05-01 00:00:00

  • Differential activation of ABI3 and LEA genes upon plant parasitic nematode infection.

    abstract::SUMMARY Promoter activity of ABI3 and of three LEA genes was monitored in Arabidopsis transgenics infected with Heterodera schachtii and Meloidogyne incognita. ABI3::GUS expression was induced (in four different promoter deletion constructs) during early infection stages with H. schachtii. Similar GUS expression patte...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2005.00283.x

    authors: DE Meutter J,Robertson L,Parcy F,Mena M,Fenoll C,Gheysen G

    更新日期:2005-05-01 00:00:00

  • De novo generation of Lettuce infectious yellows virus defective RNAs in protoplasts.

    abstract::Summary Lettuce infectious yellows virus (LIYV)-infected plants contain a heterogeneous population of defective RNAs (D RNAs) derived from LIYV genomic RNA 2. To partly address how LIYV D RNAs are generated, in vitro synthesized transcripts corresponding to the LIYV genomic RNAs 1 and 2 were inoculated to protoplasts,...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2002.00125.x

    authors: Rubio L,Tian T,Yeh HH,Livieratos Y,Falk BW

    更新日期:2002-09-01 00:00:00

  • Type III secretion inhibitors for the management of bacterial plant diseases.

    abstract::The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The majo...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12736

    authors: Puigvert M,Solé M,López-Garcia B,Coll NS,Beattie KD,Davis RA,Elofsson M,Valls M

    更新日期:2019-01-01 00:00:00

  • Distribution and pathway for phloem-dependent movement of Melon necrotic spot virus in melon plants.

    abstract::The translocation of Melon necrotic spot virus (MNSV) within tissues of inoculated and systemically infected Cucumis melo L. 'Galia' was studied by tissue-printing and in situ hybridization techniques. The results were compatible with the phloem vascular components being used to spread MNSV systemically by the same as...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2008.00474.x

    authors: Gosalvez-Bernal B,Genoves A,Navarro JA,Pallas V,Sanchez-Pina MA

    更新日期:2008-07-01 00:00:00

  • Analysis of Tomato spotted wilt virus NSs protein indicates the importance of the N-terminal domain for avirulence and RNA silencing suppression.

    abstract::Recently, Tomato spotted wilt virus (TSWV) nonstructural protein NSs has been identified unambiguously as an avirulence (Avr) determinant for Tomato spotted wilt (Tsw)-based resistance. The observation that NSs from two natural resistance-breaking isolates had lost RNA silencing suppressor (RSS) activity and Avr sugge...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12082

    authors: de Ronde D,Pasquier A,Ying S,Butterbach P,Lohuis D,Kormelink R

    更新日期:2014-02-01 00:00:00

  • A functionally conserved Zn2 Cys6 binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host-specific virulence of two major Pleosporales fungal pathogens of wheat.

    abstract::The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector-dominant host sensitivity gene interactions. The three best-characterized effector-sensitivity gene systems are SnToxA-Tsn1, SnTox1-Snn1 ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12511

    authors: Rybak K,See PT,Phan HT,Syme RA,Moffat CS,Oliver RP,Tan KC

    更新日期:2017-04-01 00:00:00

  • A curious case of resistance to a new encounter pathogen: myrtle rust in Australia.

    abstract::Resistance genes (R genes) in plants mediate a highly specific response to microbial pathogens, often culminating in localized cell death. Such resistance is generally pathogen race specific and believed to be the result of evolutionary selection pressure. Where a host and pathogen do not share an evolutionary history...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12331

    authors: Tobias PA,Guest DI,Külheim C,Hsieh JF,Park RF

    更新日期:2016-06-01 00:00:00

  • Penicillium expansum: biology, omics, and management tools for a global postharvest pathogen causing blue mould of pome fruit.

    abstract::Blue mould, caused primarily by Penicillium expansum, is a major threat to the global pome fruit industry, causing multimillion-dollar losses annually. The blue mould fungus negatively affects fruit quality, thereby reducing fresh fruit consumption, and significantly contributes to food loss. P. expansum also produces...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12990

    authors: Luciano-Rosario D,Keller NP,Jurick WM 2nd

    更新日期:2020-11-01 00:00:00

  • Transient expression of a vacuolar peroxidase increases susceptibility of epidermal barley cells to powdery mildew.

    abstract::summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated us...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1464-6722.2001.00079.x

    authors: Kristensen BK,Ammitzbøll H,Rasmussen SK,Nielsen KA

    更新日期:2001-11-01 00:00:00