Abstract:
:The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The major virulence determinant of most animal and plant bacterial pathogens is the type III secretion system (T3SS). In this work, we screened nine plant extracts and 12 isolated compounds-including molecules effective against human pathogens-for their capacity to inhibit the T3SS of plant pathogens and for their applicability as virulence inhibitors for crop protection. The screen was performed using a luminescent reporter system developed in the model pathogenic bacterium Ralstonia solanacearum. Five synthetic molecules, one natural product and two plant extracts were found to down-regulate T3SS transcription, most through the inhibition of the regulator hrpB. In addition, for three of the molecules, corresponding to salicylidene acylhydrazide derivatives, the inhibitory effect caused a dramatic decrease in the secretion capacity, which was translated into impaired plant responses. These candidate virulence inhibitors were then tested for their ability to protect plants. We demonstrated that salicylidene acylhydrazides can limit R. solanacearum multiplication in planta and protect tomato plants from bacterial speck caused by Pseudomonas syringae pv. tomato. Our work validates the efficiency of transcription reporters to discover compounds or natural product extracts that can be potentially applied to prevent bacterial plant diseases.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Puigvert M,Solé M,López-Garcia B,Coll NS,Beattie KD,Davis RA,Elofsson M,Valls Mdoi
10.1111/mpp.12736subject
Has Abstractpub_date
2019-01-01 00:00:00pages
20-32issue
1eissn
1464-6722issn
1364-3703journal_volume
20pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mpp.12367
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