Abstract:
:SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expression profiling of various life stages of the potato cyst nematode Globodera rostochiensis revealed a novel pectate lyase gene (Gr-pel2, 759 bp). The Gr-PEL2 protein showed highest similarity to pectate lyases from the facultative plant-parasitic nematodes Bursaphelenchus mucronatus and B. xylophilus and the soil-inhabiting saprophytic Streptomyces and Frankia species (i.e. 40-42% identity and 58-60% similarity), whereas only a remote relatedness to the previously identified Gr-PEL1 was observed (i.e. 28% identity and 43% similarity). Transient expression of Gr-pel2 in leaves of Nicotiana benthamiana resulted in severe malformations of the infiltrated tissues, not relating to maceration and soft rot symptoms. Ca(2+) is known to be essential for pectate lyase activity, and the most likely calcium-binding site was identified in the Gr-PEL2 protein by combining homology modelling of the three-dimensional structure, site-directed mutagenesis and transient expression in leaves. A highly charged cleft in Gr-PEL2, which is likely to be involved in substrate binding and which is also significantly more hydrophobic in Gr-PEL1, was shown to be essential for protein activity. Our results underline the broad spectrum of pectate lyases and cell-wall-degrading enzymes necessary for successful parasitism by cyst nematodes.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Kudla U,Milac AL,Qin L,Overmars H,Roze E,Holterman M,Petrescu AJ,Goverse A,Bakker J,Helder J,Smant Gdoi
10.1111/j.1364-3703.2007.00394.xsubject
Has Abstractpub_date
2007-05-01 00:00:00pages
293-305issue
3eissn
1464-6722issn
1364-3703pii
MPP394journal_volume
8pub_type
杂志文章abstract::The ascomycete Sclerotinia sclerotiorum is a necrotrophic plant pathogen with an extremely broad host range. It causes stem rot in Camelina sativa, a crucifer with great potential as an alternative oilseed crop. Lignification is a common phenomenon in the expression of resistance against necrotrophs, but the molecular...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00798.x
更新日期:2012-10-01 00:00:00
abstract::SUMMARY The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was develope...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00434.x
更新日期:2007-11-01 00:00:00
abstract::The complicated interplay of plant-pathogen interactions occurs on multiple levels as pathogens evolve to constantly evade the immune responses of their hosts. Many economically important crops fall victim to filamentous pathogens that produce small proteins called effectors to manipulate the host and aid infection/co...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12980
更新日期:2020-10-01 00:00:00
abstract::Tobacco vein banding mosaic virus (TVBMV) is a potyvirus which mainly infects solanaceous crops. The helper component proteinase (HCpro) of a potyvirus is an RNA silencing suppressor protein and determines the severity of disease symptoms caused by different potyviruses, including TVBMV. It has been shown that substit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12471
更新日期:2017-10-01 00:00:00
abstract::Plant immunity is often negatively impacted by heat stress. However, the underlying molecular mechanisms remain poorly characterized. Based on a genome-wide association mapping approach, this study aims to identify in Arabidopsis thaliana the genetic bases of robust resistance mechanisms to the devastating pathogen Ra...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12964
更新日期:2020-11-01 00:00:00
abstract:SUMMARY:To acquire iron from plant hosts, fungal pathogens have evolved at least two pathways for iron uptake. One system is hinged on the secretion and subsequent uptake of low-molecular-weight iron chelators termed siderophores, while the other uses cell-surface reductases to solubilize ferric iron by reducing it to ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00401.x
更新日期:2007-07-01 00:00:00
abstract::The tangerine pathotype of the ascomycete fungus Alternaria alternata is the causal agent of citrus brown spot, which can result in significant losses of both yield and marketability for tangerines worldwide. A conditionally dispensable chromosome (CDC), which harbours the host-selective ACT toxin gene cluster, is req...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12848
更新日期:2019-10-01 00:00:00
abstract::SUMMARY Recent studies have shown that resistance in several dicotyledonous plants to viruses in the genus Potyvirus is controlled by recessive alleles of the plant translation initiation factor eIF4E or eIF(iso)4E genes. Here we provide evidence that the barley rym4 gene locus, controlling immunity to viruses in the ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00294.x
更新日期:2005-07-01 00:00:00
abstract::Summary Suppression Subtractive Hybridization (SSH) was applied in a search for genes induced during the compatible interaction between Phytophthora infestans and potato. Using potato leaves that had been treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methylester (BTH) as the control tissue, a low redundanc...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2001.00059.x
更新日期:2001-05-01 00:00:00
abstract:TAXONOMY:Iris yellow spot virus (IYSV) is in the genus Tospovirus, family Bunyaviridae, with a single-stranded, tri-segmented RNA genome with an ambisense genome organization. Members of the other genera in the family infect predominantly vertebrates and insects. GEOGRAPHICAL DISTRIBUTION:IYSV is present in most Alliu...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12177
更新日期:2015-04-01 00:00:00
abstract::Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, probably together with commercial cultivars. Here, we present data indicating that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12589
更新日期:2018-05-01 00:00:00
abstract::summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated us...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00079.x
更新日期:2001-11-01 00:00:00
abstract::SUMMARY Pathogenic fungi have successfully attacked a wide range of hosts, which has forced them into ambient-adaptation. pH is one of the major ambient traits affecting the activity of pathogenicity factors secreted by the pathogen, hence, a pH sensing-response system was developed to enable the pathogen to tailor it...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00196.x
更新日期:2003-11-01 00:00:00
abstract::A high-throughput RNA-mediated gene silencing system was developed for Cochliobolus sativus (anamorph: Bipolaris sorokiniana), the causal agent of spot blotch, common root rot and black point in barley and wheat. The green fluorescent protein gene (GFP) and the proteinaceous host-selective toxin gene (ToxA) were first...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00666.x
更新日期:2011-04-01 00:00:00
abstract::To survive, plants possess elaborate defence mechanisms to protect themselves against virus or pathogen invasion. Recent studies have suggested that plant mitochondria may play an important role in host defence responses to biotic stresses. In contrast with animal mitochondria, plant mitochondria possess a unique resp...
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pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2010.00615.x
更新日期:2010-05-01 00:00:00
abstract::Several genes in the Agrobacterium tumefaciens transferred (T)-DNA encode proteins that are involved in developmental alterations, leading to the formation of tumours in infected plants. We investigated the role of the protein encoded by the Atu6002 gene, the function of which is completely unknown. Atu6002 expression...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12088
更新日期:2014-04-01 00:00:00
abstract::Protein kinases have been implicated in the regulation of many processes that guide pathogen development throughout the course of infection. A survey of the Sclerotinia sclerotiorum genome for genes encoding proteins containing the highly conserved eukaryotic protein kinase (ePK) domain, the largest protein kinase sup...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12321
更新日期:2016-05-01 00:00:00
abstract::Meloidogyne enterolobii is one of the most important plant-parasitic nematodes that can overcome the Mi-1 resistance gene and damage many economically important crops. Translationally controlled tumour protein (TCTP) is a multifunctional protein that exists in various eukaryotes and plays an important role in parasiti...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12374
更新日期:2017-01-01 00:00:00
abstract::Coffee (Coffea arabica L.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus Hemileia vastatrix. The transcriptome of H. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed dur...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00723.x
更新日期:2012-01-01 00:00:00
abstract::Resistance genes (R genes) in plants mediate a highly specific response to microbial pathogens, often culminating in localized cell death. Such resistance is generally pathogen race specific and believed to be the result of evolutionary selection pressure. Where a host and pathogen do not share an evolutionary history...
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pub_type: 杂志文章,评审
doi:10.1111/mpp.12331
更新日期:2016-06-01 00:00:00
abstract::In order to study the antiviral effect of the oligo-sulphated galactan Poly-Ga, the leaves of tobacco plants Xhanti(NN) were sprayed with water (control), with increasing concentrations of Poly-Ga, for increasing numbers of treatments or cultivated for increasing times after treatment. Control and treated plants were ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00685.x
更新日期:2011-06-01 00:00:00
abstract::Xanthomonas albilineans, the causal agent of sugarcane leaf scald, is a bacterial plant pathogen that is mainly spread by infected cuttings and contaminated harvesting tools. However, some strains of this pathogen are known to be spread by aerial means and are able to colonize the phyllosphere of sugarcane before ente...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12276
更新日期:2016-02-01 00:00:00
abstract::Burkholderia gladioli is a causal agent of bacterial panicle blight and sheath/grain browning in rice in many countries. Many strains produce the yellow pigment toxoflavin, which is highly toxic to plants, fungi, animals and microorganisms. Although there have been several studies on the toxoflavin biosynthesis system...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12262
更新日期:2016-01-01 00:00:00
abstract::SUMMARY Lipopolysaccharides (LPS) are indispensable cell surface components of Gram-negative bacteria and have diverse roles in plant-microbe interactions. Treatment of Nicotiana tabacum with the LPS of an endophytic strain of Burkholderia cepacia results in an enhanced defensive capacity in the tissue. In this study ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00234.x
更新日期:2004-07-01 00:00:00
abstract::Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) causes severe root rot and wilt in several cucurbit species, including cucumber, melon, and watermelon. Previously, a pathogenicity chromosome, chrRC , was identified in Forc. Strains that were previously nonpathogenic could infect multiple cucurbit species after ob...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12927
更新日期:2020-06-01 00:00:00
abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease in a wide range of plants via a unique interkingdom DNA transfer from bacterial cells into the plant genome. Agrobacterium tumefaciens is capable of transferring its T-DNA into different plant parts at different developmental stages for transient and ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12672
更新日期:2018-03-02 00:00:00
abstract::Genetic and genomics tools to characterize host-pathogen interactions are disproportionately directed to the host because of the focus on resistance. However, understanding the genetics of pathogen virulence is equally important and has been limited by the high cost of de novo genotyping of species with limited marker...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12214
更新日期:2015-08-01 00:00:00
abstract::SUMMARY Phytopathogenic fungi must feed on their hosts to propagate and cause disease. Their ability to access the rich nutrient supply offered by living plants is one of the most obvious properties that distinguish pathogens from saprophytes. Successful invasion by pathogens depends as much on their ability to utiliz...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00161.x
更新日期:2003-05-01 00:00:00
abstract::Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is not only a disease devastating rice production worldwide, but also an ideal model system for the study of the interaction between plants and their bacterial pathogens. The rice near-isogenic line (NIL) CBB23, derived from a cross between a wild r...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12092
更新日期:2014-05-01 00:00:00
abstract::SUMMARY Transgenic peas containing a copy of the Pea seed-borne mosaic virus (PSbMV) isolate DPD1 NIb sequence develop a 'recovery' phenotype when challenged with either the homologous (DPD1) or a heterologous (NY) PSbMV isolate. However, the specificity of the subsequent resistance differs with respect to the initiat...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00204.x
更新日期:2004-01-01 00:00:00