Abstract:
:A high-throughput RNA-mediated gene silencing system was developed for Cochliobolus sativus (anamorph: Bipolaris sorokiniana), the causal agent of spot blotch, common root rot and black point in barley and wheat. The green fluorescent protein gene (GFP) and the proteinaceous host-selective toxin gene (ToxA) were first introduced into C. sativus via the polyethylene glycol (PEG)-mediated transformation method. Transformants with a high level of expression of GFP or ToxA were generated. A silencing vector (pSGate1) based on the Gateway cloning system was developed and used to construct RNA interference (RNAi) vectors. Silencing of GFP and ToxA in the transformants was demonstrated by transformation with the RNAi construct expressing hairpin RNA (hpRNA) of the target gene. The polyketide synthase gene (CsPKS1), involved in melanin biosynthesis pathways in C. sativus, was also targeted by transformation with the RNAi vector (pSGate1-CsPKS1) encoding hpRNA of the CsPKS1 gene. The transformants with pSGate1-CsPKS1 exhibited an albino phenotype or reduced melanization, suggesting effective silencing of the endogenous CsPKS1 in C. sativus. Sectors exhibiting the wild-type phenotype of the fungus appeared in some of the CsPKS1-silenced transformants after subcultures as a result of inactivation or deletions of the RNAi transgene. The gene silencing system established provides a useful tool for functional genomics studies in C. sativus and other filamentous fungi.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Leng Y,Wu C,Liu Z,Friesen TL,Rasmussen JB,Zhong Sdoi
10.1111/j.1364-3703.2010.00666.xsubject
Has Abstractpub_date
2011-04-01 00:00:00pages
289-98issue
3eissn
1464-6722issn
1364-3703journal_volume
12pub_type
杂志文章abstract::Sugar metabolism and sugar signalling are not only critical for plant growth and development, but are also important for stress responses. However, how sugar homeostasis is involved in plant defence against pathogen attack in the model crop rice remains largely unknown. In this study, we observed that the grains of gi...
journal_title:Molecular plant pathology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2019-04-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12990
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12736
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12583
更新日期:2018-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
doi:10.1111/mpp.12980
更新日期:2020-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00340.x
更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mpp.12964
更新日期:2020-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12109
更新日期:2014-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1364-3703.2002.00125.x
更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mpp.12366
更新日期:2016-09-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12518
更新日期:2018-02-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12764
更新日期:2019-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1364-3703.2001.00059.x
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00537.x
更新日期:2009-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00597.x
更新日期:2010-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12899
更新日期:2020-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12134
更新日期:2014-09-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00234.x
更新日期:2004-07-01 00:00:00
abstract:UNLABELLED:summary Mycosphaerella graminicola is an important pathogen of wheat, causing septoria leaf blotch disease. This review summarizes the current knowledge on disease development and control of the fungus, and discusses how molecular tools and genomics are being employed to uncover the genetic basis of pathogen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2002.00100.x
更新日期:2002-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12321
更新日期:2016-05-01 00:00:00