Abstract:
:Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a serious disease of rice. Xoo secretes a repertoire of cell wall-degrading enzymes, including cellulases, xylanases and pectinases, to degrade various polysaccharide components of the rice cell wall. A secreted Xoo cellulase, CbsA, is not only a key virulence factor of Xoo, but is also a potent inducer of innate immune responses of rice. In this study, we solved the crystal structure of the catalytic domain of the CbsA protein to a resolution of 1.86 Å. The core structure of CbsA shows a central distorted TIM barrel made up of eight β strands with N- and C-terminal loops enclosing the active site, which is a characteristic structural feature of an exoglucanase. The aspartic acid at the 131st position of CbsA was predicted to be important for catalysis and was therefore mutated to alanine to study its role in the catalysis and biological functions of CbsA. Intriguingly, the D131A CbsA mutant protein displayed the enzymatic activity of a typical endoglucanase. D131A CbsA was as proficient as wild-type (Wt) CbsA in inducing rice immune responses, but was deficient in virulence-promoting activity. This indicates that the specific exoglucanase activity of the Wt CbsA protein is required for this protein to promote the growth of Xoo in rice.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Tayi L,Kumar S,Nathawat R,Haque AS,Maku RV,Patel HK,Sankaranarayanan R,Sonti RVdoi
10.1111/mpp.12620subject
Has Abstractpub_date
2018-06-01 00:00:00pages
1364-1376issue
6eissn
1464-6722issn
1364-3703journal_volume
19pub_type
杂志文章abstract::The ascomycete Sclerotinia sclerotiorum is a necrotrophic plant pathogen with an extremely broad host range. It causes stem rot in Camelina sativa, a crucifer with great potential as an alternative oilseed crop. Lignification is a common phenomenon in the expression of resistance against necrotrophs, but the molecular...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00798.x
更新日期:2012-10-01 00:00:00
abstract::The complicated interplay of plant-pathogen interactions occurs on multiple levels as pathogens evolve to constantly evade the immune responses of their hosts. Many economically important crops fall victim to filamentous pathogens that produce small proteins called effectors to manipulate the host and aid infection/co...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12980
更新日期:2020-10-01 00:00:00
abstract::Using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) marker analyses, the genetic structure of selected South African wheat stem rust races was compared with Ug99. SSR analysis divided the population into two distinct groups with 24.5% similarity between them. A local race, UVPgt55 (Nor...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00525.x
更新日期:2009-03-01 00:00:00
abstract:TAXONOMY:Superkingdom Eukaryota; Kingdom Metazoa; Phylum Nematoda; Class Chromadorea; Order Rhabditida; Suborder Tylenchina; Infraorder Tylenchomorpha; Superfamily Tylenchoidea; Family Pratylenchidae; Subfamily Radopholinae; Genus Radopholus. PHYSICAL PROPERTIES:Microscopic unsegmented worm; migratory endoparasite of ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00614.x
更新日期:2010-05-01 00:00:00
abstract::Erwinia amylovora is the causal agent of fire blight, one of the most devastating diseases of apple and pear. Erwinia amylovora is thought to have originated in North America and has now spread to at least 50 countries worldwide. An understanding of the diversity of the pathogen population and the transmission to diff...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12647
更新日期:2018-07-01 00:00:00
abstract::The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was construct...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00782.x
更新日期:2012-09-01 00:00:00
abstract::Hormones are tuners of plant responses to biotic and abiotic stresses. They are involved in various complicated networks, through which they modulate responses to different stimuli. Four hormones primarily regulate plant defence to pathogens: salicylic acid (SA), jasmonic acid (JA), ethylene (Et) and abscisic acid (AB...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12204
更新日期:2015-06-01 00:00:00
abstract::SUMMARY Fusarium graminearum is the main causative agent of Fusarium head blight on small grain cereals and of ear rot on maize. The disease leads to dramatic yield losses and to an accumulation of mycotoxins. The most dominant F. graminearum mycotoxins are the trichothecenes, with deoxynivalenol and nivalenol being t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00351.x
更新日期:2006-11-01 00:00:00
abstract::Powdery mildew is a common and widespread plant disease of considerable agronomic relevance. It is caused by obligate biotrophic fungal pathogens which, in most cases, epiphytically colonize aboveground plant tissues. The disease has been typically studied as a binary interaction of the fungal pathogen with its plant ...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12771
更新日期:2019-04-01 00:00:00
abstract::Ustilago maydis is an obligate biotrophic fungal pathogen which causes common smut disease of corn. To proliferate in host tissue, U. maydis must gain access to nutrients and overcome plant defence responses, such as the production of reactive oxygen species. The elucidation of the mechanisms by which U. maydis meets ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12391
更新日期:2017-02-01 00:00:00
abstract:SUMMARY:To acquire iron from plant hosts, fungal pathogens have evolved at least two pathways for iron uptake. One system is hinged on the secretion and subsequent uptake of low-molecular-weight iron chelators termed siderophores, while the other uses cell-surface reductases to solubilize ferric iron by reducing it to ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00401.x
更新日期:2007-07-01 00:00:00
abstract::Systemic acquired resistance (SAR) is a potent plant defence system that, in response to a first contact with a plant pathogen, prepares the whole plant for subsequent attacks, so that it becomes more resistant to the same and to other pathogens. BcSpl1, a cerato-platanin family protein abundantly secreted by Botrytis...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00842.x
更新日期:2013-02-01 00:00:00
abstract::SUMMARY Brachiaria is a pan-tropical genus of grasses with about 100 species. The fungus Acremonium implicatum can develop an endophytic association that is mutually beneficial with Brachiaria species. We developed a polymerase chain reaction (PCR)-based method by first amplifying DNA from A. implicatum isolates using...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00157.x
更新日期:2003-03-01 00:00:00
abstract::SUMMARY Recent studies have shown that resistance in several dicotyledonous plants to viruses in the genus Potyvirus is controlled by recessive alleles of the plant translation initiation factor eIF4E or eIF(iso)4E genes. Here we provide evidence that the barley rym4 gene locus, controlling immunity to viruses in the ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00294.x
更新日期:2005-07-01 00:00:00
abstract::To deploy durable plant resistance, we must understand its underlying molecular mechanisms. Type III effectors (T3Es) and their recognition play a central role in the interaction between bacterial pathogens and crops. We demonstrate that the Ralstonia solanacearum species complex (RSSC) T3E ripAX2 triggers specific re...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12724
更新日期:2018-11-01 00:00:00
abstract::RXLR effectors encoded by Phytophthora species play a central role in pathogen-plant interactions. An understanding of the biological functions of RXLR effectors is conducive to the illumination of the pathogenic mechanisms and the development of disease control strategies. However, the virulence function of Phytophth...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12760
更新日期:2019-03-01 00:00:00
abstract::Botrytis cinerea is a necrotrophic fungus that infects a wide range of fruit, vegetable and flower crops. Penetration of the host cuticle occurs via infection structures that are formed in response to appropriate plant surface signals. The differentiation of these structures requires a highly conserved mitogen-activat...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12234
更新日期:2015-10-01 00:00:00
abstract::A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslat...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00597.x
更新日期:2010-03-01 00:00:00
abstract::Summary Turnip crinkle virus encodes two proteins, p8 and p9, that are both required for cell-to-cell movement. The p8 movement protein has been demonstrated to bind RNA in a cooperative manner, although, similar to many other plant virus movement proteins, it contains no canonical RNA binding domain(s). However, thre...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2001.00048.x
更新日期:2001-01-01 00:00:00
abstract::Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen that infects over 400 species of plants worldwide. Reactive oxygen species (ROS) modulations are critical for the pathogenic development of S. sclerotiorum. The fungus applies enzymatic and non-enzymatic antioxidants to cope with the oxidative stre...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12801
更新日期:2019-07-01 00:00:00
abstract::After invasion into intercellular spaces of tomato plants, the soil-borne, plant-pathogenic Ralstonia solanacearum strain OE1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mBFs) on tomato cells, leading to its virulence. The strain OE1-1 produces aryl-furanone secondary metabolites, ralfuranones (A, B, J, ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12583
更新日期:2018-04-01 00:00:00
abstract::It is hypothesized that the virulence of phytopathogenic fungi is mediated through the secretion of small effector proteins that interfere with the defence responses of the host plant. In Fusarium oxysporum, one family of effectors, the Secreted In Xylem (SIX) genes, has been identified. We sought to characterize the ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12594
更新日期:2018-05-01 00:00:00
abstract::SUMMARY Two of the currently widely accepted species in the section Nigrescentia of the genus Verticillium are major plant pathogens inducing wilt diseases in a wide range of mainly dicotyledonous hosts. Three species closely related to these two are less important wilt pathogens and soil saprophytes. A sixth species,...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00172.x
更新日期:2003-07-01 00:00:00
abstract::SUMMARY Here, we consider the barley powdery mildew fungus, Blumeria graminis (DC Speer) f.sp. hordei (Marchal), and review recent research which has added to our understanding of the biology and molecular biology which underpins the asexual life cycle of this potentially devastating pathogen. We focus on the early st...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00303.x
更新日期:2005-09-01 00:00:00
abstract::The translocation of Melon necrotic spot virus (MNSV) within tissues of inoculated and systemically infected Cucumis melo L. 'Galia' was studied by tissue-printing and in situ hybridization techniques. The results were compatible with the phloem vascular components being used to spread MNSV systemically by the same as...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00474.x
更新日期:2008-07-01 00:00:00
abstract::In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear to have a negative effect on disease resistance. Using the ABA-deficient sitiens tomato (Solanum lycopersicum) mutant and different application methods of exogenous ABA, we demonstrated the influence...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00437.x
更新日期:2008-01-01 00:00:00
abstract::Genetic and genomics tools to characterize host-pathogen interactions are disproportionately directed to the host because of the focus on resistance. However, understanding the genetics of pathogen virulence is equally important and has been limited by the high cost of de novo genotyping of species with limited marker...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12214
更新日期:2015-08-01 00:00:00
abstract::Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transc...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12367
更新日期:2016-12-01 00:00:00
abstract::SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expres...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00394.x
更新日期:2007-05-01 00:00:00
abstract::Colletotrichum graminicola is a hemibiotrophic fungus that causes anthracnose leaf blight (ALB) and anthracnose stalk rot (ASR) in maize. Despite substantial economic losses caused by these diseases, the defence mechanisms against this pathogen remain poorly understood. Several hormones are suggested to aid in defence...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12924
更新日期:2020-05-01 00:00:00