Mutational analysis of Turnip crinkle virus movement protein p8.

Abstract:

:Summary Turnip crinkle virus encodes two proteins, p8 and p9, that are both required for cell-to-cell movement. The p8 movement protein has been demonstrated to bind RNA in a cooperative manner, although, similar to many other plant virus movement proteins, it contains no canonical RNA binding domain(s). However, three positively charged regions of p8 may potentially form ionic interactions with the RNA backbone. To identify functional regions of p8, a series of alanine and deletion scanning mutations were produced. The effects of these mutations were analysed using both in vitro RNA binding assays and in vivo infections of susceptible (Di-3) and resistant (Di-17) Arabidopsis thaliana plants. Several mutants that have reduced RNA binding ability were also demonstrated to be movement deficient and replication competent. Based on these results, there appear to be two regions, located between amino acids 18 and 31, and 50 and 72, that are required for RNA binding. Furthermore, additional regions (amino acids 12-15, and 34-37) appear to play a role in vivo unrelated to in vitro RNA binding activity.

journal_name

Mol Plant Pathol

authors

Akgoz M,Nguyen QN,Talmadge AE,Drainville KE,Wobbe KK

doi

10.1046/j.1364-3703.2001.00048.x

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

37-48

issue

1

eissn

1464-6722

issn

1364-3703

pii

MPP048

journal_volume

2

pub_type

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