Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation.

Abstract:

:Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High-performance thin-layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew.

journal_name

Mol Plant Pathol

authors

Li Y,Héloir MC,Zhang X,Geissler M,Trouvelot S,Jacquens L,Henkel M,Su X,Fang X,Wang Q,Adrian M

doi

10.1111/mpp.12809

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

1037-1050

issue

8

eissn

1464-6722

issn

1364-3703

journal_volume

20

pub_type

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