Abstract:
:Because of their highly ordered structure, mature viroid RNA molecules are assumed to be resistant to degradation by RNA interference (RNAi). In this article, we report that transgenic tomato plants expressing a hairpin RNA (hpRNA) construct derived from Potato spindle tuber viroid (PSTVd) sequences exhibit resistance to PSTVd infection. Resistance seems to be correlated with high-level accumulation of hpRNA-derived short interfering RNAs (siRNAs) in the plant. Thus, although small RNAs produced by infecting viroids [small RNAs of PSTVd (srPSTVds)] do not silence viroid RNAs efficiently to prevent their replication, hpRNA-derived siRNAs (hp-siRNAs) appear to effectively target the mature viroid RNA. Genomic mapping of the hp-siRNAs revealed an unequal distribution of 21- and 24-nucleotide siRNAs of both (+)- and (-)-strand polarities along the PSTVd genome. These data suggest that RNAi can be employed to engineer plants for viroid resistance, as has been well established for viruses.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Schwind N,Zwiebel M,Itaya A,Ding B,Wang MB,Krczal G,Wassenegger Mdoi
10.1111/j.1364-3703.2009.00546.xsubject
Has Abstractpub_date
2009-07-01 00:00:00pages
459-69issue
4eissn
1464-6722issn
1364-3703pii
MPP546journal_volume
10pub_type
杂志文章abstract::SUMMARY Fusarium graminearum is the main causative agent of Fusarium head blight on small grain cereals and of ear rot on maize. The disease leads to dramatic yield losses and to an accumulation of mycotoxins. The most dominant F. graminearum mycotoxins are the trichothecenes, with deoxynivalenol and nivalenol being t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00351.x
更新日期:2006-11-01 00:00:00
abstract::Xanthomonas albilineans, the causal agent of sugarcane leaf scald, is a bacterial plant pathogen that is mainly spread by infected cuttings and contaminated harvesting tools. However, some strains of this pathogen are known to be spread by aerial means and are able to colonize the phyllosphere of sugarcane before ente...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12276
更新日期:2016-02-01 00:00:00
abstract::Summary Functional coat protein (CP) is important for host plant infection by monopartite geminiviruses. We identified a proline-cysteine-lysine (PCK) motif at amino acids 180-182 of the maize streak virus (MSV) CP that is conserved in most of the cereal-infecting Mastreviruses. Substitution of the lysine (K) with a v...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00068.x
更新日期:2001-07-01 00:00:00
abstract::Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) causes severe root rot and wilt in several cucurbit species, including cucumber, melon, and watermelon. Previously, a pathogenicity chromosome, chrRC , was identified in Forc. Strains that were previously nonpathogenic could infect multiple cucurbit species after ob...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12927
更新日期:2020-06-01 00:00:00
abstract::Abstract To test whether carbohydrates may play a signalling function during plant pathogenesis, we investigated the interaction between tobacco and potato virus Y (PVY(N)). Four days after PVY(N) infection, leaves started to accumulate soluble sugars and leaf photosynthesis decreased. The accumulation of soluble suga...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00007.x
更新日期:2000-01-01 00:00:00
abstract::The complicated interplay of plant-pathogen interactions occurs on multiple levels as pathogens evolve to constantly evade the immune responses of their hosts. Many economically important crops fall victim to filamentous pathogens that produce small proteins called effectors to manipulate the host and aid infection/co...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12980
更新日期:2020-10-01 00:00:00
abstract::Pectobacterium carotovorum has an incomplete Entner-Doudoroff (ED) pathway, including enzyme 2-keto-3-deoxy-6-phosphogluconate aldolase (Eda) but lacking phosphogluconate dehydratase (Edd), while P. atrosepticum (Pba) has a complete pathway. To understand the role of the ED pathway in Pectobacterium infection, mutants...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.13025
更新日期:2021-02-01 00:00:00
abstract::SUMMARY The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was develope...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00434.x
更新日期:2007-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00437.x
更新日期:2008-01-01 00:00:00
abstract::After invasion into intercellular spaces of tomato plants, the soil-borne, plant-pathogenic Ralstonia solanacearum strain OE1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mBFs) on tomato cells, leading to its virulence. The strain OE1-1 produces aryl-furanone secondary metabolites, ralfuranones (A, B, J, ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12583
更新日期:2018-04-01 00:00:00
abstract::SUMMARY Pathogenic fungi have successfully attacked a wide range of hosts, which has forced them into ambient-adaptation. pH is one of the major ambient traits affecting the activity of pathogenicity factors secreted by the pathogen, hence, a pH sensing-response system was developed to enable the pathogen to tailor it...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00196.x
更新日期:2003-11-01 00:00:00
abstract:TAXONOMY:Soybean mosaic virus (SMV) is a species within the genus Potyvirus, family Potyviridae, which includes almost one-quarter of all known plant RNA viruses affecting agriculturally important plants. The Potyvirus genus is the largest of all genera of plant RNA viruses with 160 species. PARTICLE:The filamentous p...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12644
更新日期:2018-07-01 00:00:00
abstract::The basal ascomycetes in genus Taphrina have strict host specificity and coevolution with their host plants, making them appealing models for studying the genomic basis of ecological divergence and host adaption. We therefore performed genome sequencing and comparative genomics of different Taphrina species with disti...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12899
更新日期:2020-03-01 00:00:00
abstract::SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expres...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00394.x
更新日期:2007-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12485
更新日期:2017-12-01 00:00:00
abstract::Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12252
更新日期:2015-12-01 00:00:00
abstract::The fungal cereal pathogen Fusarium graminearum produces deoxynivalenol (DON) during infection. The mycotoxin DON is associated with Fusarium head blight (FHB), a disease that can cause vast grain losses. Whilst investigating the suitability of Brachypodium distachyon as a model for spreading resistance to F. graminea...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12203
更新日期:2015-06-01 00:00:00
abstract::Olive anthracnose causes fruit rot leading to its drop or mummification, resulting in yield losses and the degradation of oil quality. TAXONOMY AND DISTRIBUTION:The disease is caused by diverse species of Colletotrichum, mostly clustering in the C. acutatum species complex. Colletotrichum nymphaeae and C. godetiae ar...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12676
更新日期:2018-03-08 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12560
更新日期:2018-04-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a serious disease of rice. Xoo secretes a repertoire of cell wall-degrading enzymes, including cellulases, xylanases and pectinases, to degrade various polysaccharide components of the rice cell wall. A secreted Xoo cellulase, CbsA, is not only a key virulen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12620
更新日期:2018-06-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12981
更新日期:2020-10-01 00:00:00
abstract::summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated us...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00079.x
更新日期:2001-11-01 00:00:00
abstract::Efficient nutrient acquisition is critical to the fitness of plant pathogens. To address how the late blight agent Phytophthora infestans adapts to nutrients offered by its hosts, genes in glycolytic, gluconeogenic and amino acid pathways were mined from its genome and their expression in different plant tissues and a...
journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12577
更新日期:2018-04-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00419.x
更新日期:2007-09-01 00:00:00
abstract::Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12809
更新日期:2019-08-01 00:00:00
abstract::MicroRNA-like RNAs (milRNAs) post-transcriptionally down-regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil-2, had >100 transcripts per million in conidia, myc...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12859
更新日期:2019-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12381
更新日期:2017-01-01 00:00:00
abstract::Gene silencing exists in eukaryotic organisms as a conserved regulation of the gene expression mechanism. In general, small RNAs (sRNAs) are produced within the eukaryotic cells and incorporated into an RNA-induced silencing complex (RISC) within cells. However, exogenous sRNAs, once delivered into cells, can also sil...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12863
更新日期:2019-11-01 00:00:00
abstract::A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contrib...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00820.x
更新日期:2012-12-01 00:00:00