Abstract:
:SUMMARY We sought to identify Fusarium oxysporum f. sp. vasinfectum (Fov) genes that may be associated with pathogenicity. Initially we utilized microarray and Q-PCR technology to identify Fov genes expressed in root and hypocotyl tissues during a compatible infection of cotton. We identified 218 fungal clones representing 174 Fov non-redundant genes as expressed in planta. The majority of the expressed sequences were expressed in infected roots, with only six genes detected in hypocotyl tissue. The Fov genes identified were predominately of unknown function or associated with fungal growth and energy production. We then analysed the expression of the identified fungal genes in infected roots and in saprophytically grown mycelia and identified 11 genes preferentially expressed in plant tissue. A putative oxidoreductase gene (with homology to AtsC) was found to be highly preferentially expressed in planta. In Agrobacterium tumefaciens, AtsC is associated with virulence. Inoculation of a susceptible and a partially resistant cotton cultivar with either a pathogenic or a non-pathogenic isolate of Fov revealed that the expression of the Fov AtsC homologue was associated with pathogenicity and disease symptom formation.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
McFadden HG,Wilson IW,Chapple RM,Dowd Cdoi
10.1111/j.1364-3703.2006.00327.xsubject
Has Abstractpub_date
2006-03-01 00:00:00pages
87-101issue
2eissn
1464-6722issn
1364-3703pii
MPP327journal_volume
7pub_type
杂志文章abstract::Membrane trafficking is vital to plant development and adaptation to the environment. It is suggested that post-Golgi vesicles and multivesicular bodies are essential for plant defence against directly penetrating fungal parasites at the cell wall. However, the actual plant proteins involved in membrane transport for ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00846.x
更新日期:2013-04-01 00:00:00
abstract:TAXONOMY:Superkingdom Eukaryota; Kingdom Metazoa; Phylum Nematoda; Class Chromadorea; Order Rhabditida; Suborder Tylenchina; Infraorder Tylenchomorpha; Superfamily Tylenchoidea; Family Pratylenchidae; Subfamily Radopholinae; Genus Radopholus. PHYSICAL PROPERTIES:Microscopic unsegmented worm; migratory endoparasite of ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00614.x
更新日期:2010-05-01 00:00:00
abstract::The ascomycete Sclerotinia sclerotiorum is a necrotrophic plant pathogen with an extremely broad host range. It causes stem rot in Camelina sativa, a crucifer with great potential as an alternative oilseed crop. Lignification is a common phenomenon in the expression of resistance against necrotrophs, but the molecular...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00798.x
更新日期:2012-10-01 00:00:00
abstract::The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The majo...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12736
更新日期:2019-01-01 00:00:00
abstract::Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, probably together with commercial cultivars. Here, we present data indicating that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12589
更新日期:2018-05-01 00:00:00
abstract::Pattern recognition receptors (PRRs) can trigger plant immunity through the recognition of pathogen-associated molecular patterns. In this study, we report that a malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, from Haynaldia villosa putatively acts as a PRR to positively regulate powdery mildew...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12729
更新日期:2018-12-01 00:00:00
abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00212.x
更新日期:2004-03-01 00:00:00
abstract::As a result of contradictory reports, the avirulence (Avr) determinant that triggers Tsw gene-based resistance in Capsicum annuum against the Tomato spotted wilt virus (TSWV) is still unresolved. Here, the N and NSs genes of resistance-inducing (RI) and resistance-breaking (RB) isolates were cloned and transiently exp...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12016
更新日期:2013-05-01 00:00:00
abstract::Genetic and genomics tools to characterize host-pathogen interactions are disproportionately directed to the host because of the focus on resistance. However, understanding the genetics of pathogen virulence is equally important and has been limited by the high cost of de novo genotyping of species with limited marker...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12214
更新日期:2015-08-01 00:00:00
abstract::Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12252
更新日期:2015-12-01 00:00:00
abstract::The type III secretion system (T3SS) is required for Xanthomonas citri subsp. citri (Xcc) virulence by translocating effectors into host cytoplasm to promote disease development. The T3SS is controlled by the master transcriptional regulators HrpG and HrpX. While the function of HrpG and HrpX are well characterized, t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12786
更新日期:2019-05-01 00:00:00
abstract::Research has established that mutations in highly conserved amino acids of the succinate dehydrogenase (SDH) complex in various fungi confer SDH inhibitor (SDHI) resistance. For Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic fungus with a broad host range and a worldwide distribution, boscalid resistance has ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12222
更新日期:2015-09-01 00:00:00
abstract::LysR-type transcriptional regulators (LTTRs) are ubiquitous and abundant amongst bacteria and control a variety of cellular processes. Here, we investigated the effect of Rsc1880 (a putative LTTR, hereafter designated as PrhO) on the pathogenicity of Ralstonia solanacearum. Deletion of prhO substantially reduced the e...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12660
更新日期:2018-01-24 00:00:00
abstract::A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslat...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00597.x
更新日期:2010-03-01 00:00:00
abstract::Piriformospora indica is a basidiomycete of the order Sebacinales, representing a model for the study of mutualistic symbiosis and, beyond that, the plant immune system. The fungus colonizes the roots of a wide range of vascular plants, increasing their growth, seed yield and adaptation to abiotic and biotic stresses....
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2011.00764.x
更新日期:2012-06-01 00:00:00
abstract::SUMMARY The phytotoxin coronatine (COR) contributes to the virulence of Pseudomonas syringae pv. tomato (Pst) strain DC3000 on Arabidopsis thaliana and tomato. However, little is known regarding the role of COR in the virulence of DC3000 on cultivated Brassica spp. In this study, the role of COR and its precursors, co...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00372.x
更新日期:2007-01-01 00:00:00
abstract::A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contrib...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00820.x
更新日期:2012-12-01 00:00:00
abstract::Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transc...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12367
更新日期:2016-12-01 00:00:00
abstract::The tangerine pathotype of the ascomycete fungus Alternaria alternata is the causal agent of citrus brown spot, which can result in significant losses of both yield and marketability for tangerines worldwide. A conditionally dispensable chromosome (CDC), which harbours the host-selective ACT toxin gene cluster, is req...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12848
更新日期:2019-10-01 00:00:00
abstract::SUMMARY As in many fungi, asexual reproduction of Mycosphaerella graminicola in planta is a complex process that requires proper differentiation of the infectious hyphae in the substomatal cavities of foliar tissue before pycnidia with conidia can be formed. In this study, we have investigated the role of the cAMP sig...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00361.x
更新日期:2006-11-01 00:00:00
abstract::Amongst the universal diseases affecting apples, blue mould caused by Penicillium expansum is a major concern, resulting in yield and quality losses as a result of the production of the mycotoxin patulin. Despite the characterization of the patulin biosynthetic gene cluster at both the molecular and chemical levels, t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12734
更新日期:2018-12-01 00:00:00
abstract::SUMMARY Annexins are calcium-binding proteins that have been associated in plants with different biological processes such as responses to abiotic stress and early nodulation stages. Until now, the implication of annexins during plant-pathogen interactions has not been reported. Here, a novel plant annexin gene induce...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00385.x
更新日期:2007-03-01 00:00:00
abstract::Recently, Tomato spotted wilt virus (TSWV) nonstructural protein NSs has been identified unambiguously as an avirulence (Avr) determinant for Tomato spotted wilt (Tsw)-based resistance. The observation that NSs from two natural resistance-breaking isolates had lost RNA silencing suppressor (RSS) activity and Avr sugge...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12082
更新日期:2014-02-01 00:00:00
abstract::Flower malformation represented by phyllody is a common symptom of phytoplasma infection induced by a novel family of phytoplasma effectors called phyllogens. Despite the accumulation of functional and structural phyllogen information, the molecular mechanisms of phyllody have not yet been integrated with their evolut...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12981
更新日期:2020-10-01 00:00:00
abstract::SUMMARY Fusarium graminearum is the main causative agent of Fusarium head blight on small grain cereals and of ear rot on maize. The disease leads to dramatic yield losses and to an accumulation of mycotoxins. The most dominant F. graminearum mycotoxins are the trichothecenes, with deoxynivalenol and nivalenol being t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00351.x
更新日期:2006-11-01 00:00:00
abstract::Tobacco vein banding mosaic virus (TVBMV) is a potyvirus which mainly infects solanaceous crops. The helper component proteinase (HCpro) of a potyvirus is an RNA silencing suppressor protein and determines the severity of disease symptoms caused by different potyviruses, including TVBMV. It has been shown that substit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12471
更新日期:2017-10-01 00:00:00
abstract::summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated us...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00079.x
更新日期:2001-11-01 00:00:00
abstract::Successful host-pathogen interactions require the presence, maintenance and expression of gene cassettes called 'pathogenicity islands' (PAIs) and 'metabolic islands' (MAIs) in the respective pathogen. The products of these genes confer on the pathogen the means to recognize their host(s) and to efficiently evade host...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2008.00518.x
更新日期:2009-01-01 00:00:00
abstract::The expression pattern of pathogenesis-related genes PR-1, PR-2 and PR-5, considered as markers for salicylic acid (SA)-dependent systemic acquired resistance (SAR), was examined in the roots and shoots of tomato plants pre-treated with SA and subsequently infected with root-knot nematodes (RKNs) (Meloidogyne incognit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12085
更新日期:2014-04-01 00:00:00
abstract::Several genes in the Agrobacterium tumefaciens transferred (T)-DNA encode proteins that are involved in developmental alterations, leading to the formation of tumours in infected plants. We investigated the role of the protein encoded by the Atu6002 gene, the function of which is completely unknown. Atu6002 expression...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12088
更新日期:2014-04-01 00:00:00