Abstract:
:Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweet potato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including sweet potato feathery mottle virus (SPFMV; genus Potyvirus, family Potyviridae). Because no sources of true resistance to SPCSV are available in sweet potato germplasm, a pathogen-derived transgenic resistance strategy was tested as an alternative solution in this study. A Peruvian sweet potato landrace 'Huachano' was transformed with an intron-spliced hairpin construct targeting the replicase encoding sequences of SPCSV and SPFMV using an improved genetic transformation procedure with reproducible efficiency. Twenty-eight independent transgenic events were obtained in three transformation experiments using a highly virulent Agrobacterium tumefaciens strain and regeneration through embryogenesis. Molecular analysis indicated that all regenerants were transgenic, with 1-7 transgene loci. Accumulation of transgene-specific siRNA was detected in most of them. None of the transgenic events was immune to SPCSV, but ten of the 20 tested transgenic events exhibited mild or no symptoms following infection, and accumulation of SPCSV was significantly reduced. There are few previous reports of RNA silencing-mediated transgenic resistance to viruses of Closteroviridae in cultivated plants. However, the high levels of resistance to accumulation of SPCSV could not prevent development of synergistic sweet potato virus disease in those transgenic plants also infected with SPFMV.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Kreuze JF,Klein IS,Lazaro MU,Chuquiyuri WJ,Morgan GL,Mejía PG,Ghislain M,Valkonen JPdoi
10.1111/j.1364-3703.2008.00480.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
589-98issue
5eissn
1464-6722issn
1364-3703pii
MPP480journal_volume
9pub_type
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