To mate or not to mate.

abstract：:The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...

journal_title：eLife

authors： Alcock F,Stansfeld PJ,Basit H,Habersetzer J,Baker MA,Palmer T,Wallace MI,Berks BC

更新日期：2016-12-03 00:00:00

abstract：:Smoothened (Smo) inhibition by Patched (Ptch) is central to Hedgehog (Hh) signaling. Ptch, a proton driven antiporter, is required for Smo inhibition via an unknown mechanism. Hh ligand binding to Ptch reverses this inhibition and activated Smo initiates the Hh response. To determine whether Ptch inhibits Smo strictly...

journal_title：eLife

authors： Roberts B,Casillas C,Alfaro AC,Jägers C,Roelink H

更新日期：2016-08-23 00:00:00

abstract：:Building a genotype-phenotype-fitness map of adaptation is a central goal in evolutionary biology. It is difficult even when adaptive mutations are known because it is hard to enumerate which phenotypes make these mutations adaptive. We address this problem by first quantifying how the fitness of hundreds of adaptive ...

journal_title：eLife

authors： Kinsler G,Geiler-Samerotte K,Petrov DA

更新日期：2020-12-02 00:00:00

abstract：:Mammalian HMG-CoA reductase (HMGCR), the rate-limiting enzyme of the cholesterol biosynthetic pathway and the therapeutic target of statins, is post-transcriptionally regulated by sterol-accelerated degradation. Under cholesterol-replete conditions, HMGCR is ubiquitinated and degraded, but the identity of the E3 ubiqu...

journal_title：eLife

authors： Menzies SA,Volkmar N,van den Boomen DJ,Timms RT,Dickson AS,Nathan JA,Lehner PJ

更新日期：2018-12-13 00:00:00

abstract：:Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole e...

journal_title：eLife

authors： Pettinati I,Grzechnik P,Ribeiro de Almeida C,Brem J,McDonough MA,Dhir S,Proudfoot NJ,Schofield CJ

更新日期：2018-12-03 00:00:00

abstract：:Antibiotics are widely used in the treatment of bacterial infections. Although known for their microbicidal activity, antibiotics may also interfere with the host's immune system. Here, we analyzed the effects of bedaquiline (BDQ), an inhibitor of the mycobacterial ATP synthase, on human macrophages. Genome-wide gene ...

journal_title：eLife

authors： Giraud-Gatineau A,Coya JM,Maure A,Biton A,Thomson M,Bernard EM,Marrec J,Gutierrez MG,Larrouy-Maumus G,Brosch R,Gicquel B,Tailleux L

更新日期：2020-05-04 00:00:00

abstract：:In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC). Natural Arabidopsis accessions show considerable variation in vernalization. However, which aspect of the FLC repression mechanism is mos...

journal_title：eLife

authors： Hepworth J,Antoniou-Kourounioti RL,Berggren K,Selga C,Tudor EH,Yates B,Cox D,Collier Harris BR,Irwin JA,Howard M,Säll T,Holm S,Dean C

更新日期：2020-09-09 00:00:00

abstract：:Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...

journal_title：eLife

authors： Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

更新日期：2018-03-20 00:00:00

abstract：:The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...

journal_title：eLife

authors： Spira F,Cuylen-Haering S,Mehta S,Samwer M,Reversat A,Verma A,Oldenbourg R,Sixt M,Gerlich DW

更新日期：2017-11-06 00:00:00

abstract：:Orphans are genes restricted to a single phylogenetic lineage and emerge at high rates. While this predicts an accumulation of genes, the gene number has remained remarkably constant through evolution. This paradox has not yet been resolved. Because orphan genes have been mainly analyzed over long evolutionary time sc...

journal_title：eLife

authors： Palmieri N,Kosiol C,Schlötterer C

更新日期：2014-02-19 00:00:00

abstract：:The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here, we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. ...

journal_title：eLife

authors： Gehrlach DA,Weiand C,Gaitanos TN,Cho E,Klein AS,Hennrich AA,Conzelmann KK,Gogolla N

更新日期：2020-09-17 00:00:00

abstract：:Methyl-coenzyme M reductase (MCR), found in strictly anaerobic methanogenic and methanotrophic archaea, catalyzes the reversible production and consumption of the potent greenhouse gas methane. The α subunit of MCR (McrA) contains several unusual post-translational modifications, including a rare thioamidation of glyc...

journal_title：eLife

authors： Nayak DD,Mahanta N,Mitchell DA,Metcalf WW

更新日期：2017-09-07 00:00:00

abstract：:A pair of neurons is required for nematodes to be able to navigate using the Earth's magnetic field. ...

journal_title：eLife

authors： Rankin CH,Lin CH

更新日期：2015-08-05 00:00:00

abstract：:Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...

journal_title：eLife

authors： Belin BJ,Lee T,Mullins RD

更新日期：2015-08-19 00:00:00

abstract：:The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...

journal_title：eLife

authors： Schekman R,Patterson M,Watt F,Weigel D

更新日期：2012-10-15 00:00:00

abstract：:Sparse, sequential patterns of neural activity have been observed in numerous brain areas during timekeeping and motor sequence tasks. Inspired by such observations, we construct a model of the striatum, an all-inhibitory circuit where sequential activity patterns are prominent, addressing the following key challenges...

journal_title：eLife

authors： Murray JM,Escola GS

更新日期：2017-05-08 00:00:00

abstract：:The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively ...

journal_title：eLife

authors： He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

更新日期：2015-12-08 00:00:00

abstract：:While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we...

journal_title：eLife

authors： Urban L,Holzer A,Baronas JJ,Hall MB,Braeuninger-Weimer P,Scherm MJ,Kunz DJ,Perera SN,Martin-Herranz DE,Tipper ET,Salter SJ,Stammnitz MR

更新日期：2021-01-19 00:00:00

abstract：:MarR (multiple antibiotic resistance repressor) family proteins are bacterial repressors that regulate transcription in response to a wide range of chemical signals. Although specific features of MarR family function have been described, the role of atomic motions in MarRs remains unexplored thus limiting insights int...

journal_title：eLife

authors： Capdevila DA,Huerta F,Edmonds KA,Le MT,Wu H,Giedroc DP

更新日期：2018-10-17 00:00:00

abstract：:Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks of nerve tissue, dense connectomic mapping requires the identification of millions to billions of synapses. While the focus of connectomic data analysis has been on neurite reconstru...

journal_title：eLife

authors： Staffler B,Berning M,Boergens KM,Gour A,Smagt PV,Helmstaedter M

更新日期：2017-07-14 00:00:00

abstract：:Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

journal_title：eLife

authors： Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

更新日期：2018-05-30 00:00:00

abstract：:Most cellular features have a range of states, but understanding the mechanisms responsible for interspecific divergence is a challenge for evolutionary cell biology. Models are developed for the distribution of mean phenotypes likely to evolve under the joint forces of mutation and genetic drift in the face of consta...

journal_title：eLife

authors： Lynch M

更新日期：2018-06-21 00:00:00

abstract：:Most neurogenesis in the mammalian brain is completed embryonically, but in certain areas the production of neurons continues throughout postnatal life. The functional properties of mature postnatally generated neurons often match those of their embryonically produced counterparts. However, we show here that in the ol...

journal_title：eLife

authors： Galliano E,Franzoni E,Breton M,Chand AN,Byrne DJ,Murthy VN,Grubb MS

更新日期：2018-04-20 00:00:00

abstract：:Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the Dmp1Cre:Socs3f/f mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a...

journal_title：eLife

authors： Walker EC,Truong K,McGregor NE,Poulton IJ,Isojima T,Gooi JH,Martin TJ,Sims NA

更新日期：2020-05-27 00:00:00

abstract：:In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% del...

journal_title：eLife

authors： Izumo M,Pejchal M,Schook AC,Lange RP,Walisser JA,Sato TR,Wang X,Bradfield CA,Takahashi JS

更新日期：2014-12-19 00:00:00

abstract：:To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm genera...

journal_title：eLife

authors： Wang X,Hayes JA,Revill AL,Song H,Kottick A,Vann NC,LaMar MD,Picardo MC,Akins VT,Funk GD,Del Negro CA

更新日期：2014-07-15 00:00:00

abstract：:WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...

journal_title：eLife

authors： Lin Z,Yang Z,Xie R,Ji Z,Guan K,Zhang M

更新日期：2019-09-05 00:00:00

abstract：:In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We d...

journal_title：eLife

authors： Fuller GG,Han T,Freeberg MA,Moresco JJ,Ghanbari Niaki A,Roach NP,Yates JR 3rd,Myong S,Kim JK

更新日期：2020-04-16 00:00:00

abstract：:Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN. Here we show that lead pyridine-based ALLINI KF116 exhibits striking selectivity for IN tetramers versus lower order protein oligomers. I...

journal_title：eLife

authors： Koneru PC,Francis AC,Deng N,Rebensburg SV,Hoyte AC,Lindenberger J,Adu-Ampratwum D,Larue RC,Wempe MF,Engelman AN,Lyumkis D,Fuchs JR,Levy RM,Melikyan GB,Kvaratskhelia M

更新日期：2019-05-23 00:00:00

abstract：:When a plant cell divides, four related proteins control the trafficking of vesicles and ensure that cargo that is normally recycled to the plasma membrane is instead re-routed to the plane of cell division. ...

journal_title：eLife

authors： Robatzek S

更新日期：2014-04-08 00:00:00