Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties.

Abstract:

:The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by (31)P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 min for cationic moiety like methylene blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.

journal_name

Int J Biol Macromol

authors

Volkov V,Cavaco-Paulo A

doi

10.1016/j.ijbiomac.2015.12.082

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

476-86

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(15)30269-5

journal_volume

85

pub_type

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