Abstract：
:Neutrophil extracellular traps (NETs) extruded from neutrophils upon activation are composed of chromatin associated with cytosolic and granular proteins, which ensnare and kill microorganisms. This microbicidal mechanism named classical netosis has been shown to dependent on reactive oxygen species (ROS) generation by NADPH oxidase and also chromatin decondensation dependent upon the enzymes (PAD4), neutrophil elastase (NE) and myeloperoxidase (MPO). NET release also occurs through an early/rapid ROS-independent mechanism, named early/rapid vital netosis. Here we analyze the role of ROS, NE, MPO and PAD4 in the netosis stimulated by Leishmania amazonensis promastigotes in human neutrophils. We demonstrate that promastigotes induce a classical netosis, dependent on the cellular redox imbalance, as well as by a chloroamidine sensitive and elastase activity mechanism. Additionally, Leishmania also induces the early/rapid NET release occurring only 10 minutes after neutrophil-parasite interaction. We demonstrate here, that this early/rapid mechanism is dependent on elastase activity, but independent of ROS generation and chloroamidine. A better understanding of both mechanisms of NET release, and the NETs effects on the host immune system modulation, could support the development of new potential therapeutic strategies for leishmaniasis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Rochael NC,Guimarães-Costa AB,Nascimento MT,DeSouza-Vieira TS,Oliveira MP,Garcia e Souza LF,Oliveira MF,Saraiva EMdoi
10.1038/srep18302subject
Has Abstractpub_date
2015-12-17 00:00:00pages
18302issn
2045-2322pii
srep18302journal_volume
5pub_type
杂志文章abstract：:We collected data from Kailuan cohort study from 2006 to 2011 to examine whether short-term effects of ambient temperature on heart rate (HR) and blood pressure (BP) are non-linear or linear, and their potential modifying factors. The HR, BP and individual information, including basic characteristics, life style, soci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21003
更新日期：2016-02-15 00:00:00
abstract：:Pyriform sinus fistula (PSF) is a rare congenital entity. We hypothesized that clinical features, diagnosis and treatment may differ between newborns and children. 190 patients diagnosed with PSF were divided into two groups: neonatal (n = 15) and childhood (n = 175). The medical records including demographic and clin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55050-9
更新日期：2019-12-06 00:00:00
abstract：:Gold nanoparticle radiosensitization represents a novel technique in enhancement of ionising radiation dose and its effect on biological systems. Variation between theoretical predictions and experimental measurement is significant enough that the mechanism leading to an increase in cell killing and DNA damage is stil...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19442
更新日期：2016-01-20 00:00:00
abstract：:Accurate characterization of the mechanical properties of brain tissue is essential for understanding the mechanisms of traumatic brain injuries and developing protective gears or facilities. However, how storage conditions might affect the mechanical properties of brain tissue remains unclear. The objective of this s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19687-2
更新日期：2018-01-19 00:00:00
abstract：:Current chemical markers based quality assessment methods largely fail to reflect intrinsic chemical complexity and multiple mechanisms of action of botanical drugs (BD). The development of novel quality markers is greatly needed. Here we propose bioactive chemical markers (BCM), defined as a group of chemo-markers th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02305-y
更新日期：2017-05-25 00:00:00
abstract：:Though cognitive function is proven to be an independent predictor of survival in patients with intrinsic brain tumors, cognitive functions are still rarely considered. Aim of this study was to assess neurocognitive function and to identify risk factors for neurocognitive deficits. 103 patients with primary neuroepith...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17833-w
更新日期：2017-12-19 00:00:00
abstract：:The phase behaviour of a system composed of amino acid-based surfactant (sodium N-lauroylsarcosinate hydrate), 1-decanol and deionised water was investigated for vesicle formation. Changing the molar ratio of the amphiphiles, two important aggregate structures were observed in the aqueous corner of the phase diagram. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00071
更新日期：2011-01-01 00:00:00
abstract：:A top-down scalable method to produce flexible water and blood repellent tubes is introduced. The method is based on replication of overhanging nanostructures from an aluminum tube template to polydimethylsiloxane (PDMS) via atomic layer deposition (ALD) assisted sacrificial etching. The nanostructured PDMS/titania tu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16369-3
更新日期：2017-11-22 00:00:00
abstract：:Growing evidence indicates that nomogram combined with the biomarkers of systemic inflammation response could provide more accurate prediction than conventional staging systems in tumors. This study aimed to establish an effective prognostic nomogram for resectable thoracic esophageal squamous cell carcinoma (ESCC) ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18811
更新日期：2015-12-22 00:00:00
abstract：:The structural characterization correlated to the processing control of hierarchical structure of planar heterojunction perovskite layer is still incomplete due to the limitations of conventional microscopy and X-ray diffraction. This present study performed the simultaneously grazing-incidence small-angle scattering ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13657
更新日期：2015-09-04 00:00:00
abstract：:Diapause, a programmed developmental arrest, is common in insects, enabling them to survive adverse seasons. It is well established that pupal diapause is regulated by ecdysteroids secreted by the prothoracic glands (PGs), with cessation of ecdysteroid secretion after pupal ecdysis leading to pupal diapause. A major f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41651
更新日期：2017-01-31 00:00:00
abstract：:In glaucoma, retinal ganglion cells degenerate progressively, leading to visual field loss and blindness. Presently, the only treatment strategy for glaucoma is lowering the intraocular pressure. However, there are cases in which patients develop progressive visual field loss even though their intraocular pressures ar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78731-2
更新日期：2020-12-10 00:00:00
abstract：:A major translational challenge in the fields of therapeutic angiogenesis and regenerative medicine is the need to create functional microvasculature. The purpose of this study was to assess whether a potentially autologous endothelial cell (EC) source derived from human induced pluripotent stem cells (iPSC-ECs) can f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20966-1
更新日期：2018-02-08 00:00:00
abstract：:Deregulated expression of microRNAs has been associated with angiogenesis. Studying the miRNome of locally advanced breast tumors we unsuspectedly found a dramatically repression of miR-204, a small non-coding RNA with no previous involvement in tumor angiogenesis. Downregulation of miR-204 was confirmed in an indepen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34504
更新日期：2016-10-05 00:00:00
abstract：:Gestational diabetes mellitus (GDM) is a common health problem during pregnancy and its prevalence is increasing globally, especially in China. The aim of this study was to investigate socioeconomic, environmental and lifestyle factors associated with GDM in Chinese women. A matched pair case-control study was conduct...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26412-6
更新日期：2018-05-25 00:00:00
abstract：:The vertebrate gut is host to large communities of bacteria, and one of the beneficial contributions of this commensal gut microbiota is the increased nutritional gain from feed components that the host cannot degrade on its own. Fish larvae of similar age and under the same rearing conditions often diverge with regar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21192
更新日期：2016-02-15 00:00:00
abstract：:Nanostructure arrays such as nanowire, nanopillar, and nanocone arrays have been proposed to be promising antireflection structures for photovoltaic applications due to their great light trapping ability. In this paper, the optical properties of Si nanopillar and nanocone arrays in visible and infrared region were stu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07810
更新日期：2015-01-15 00:00:00
abstract：:Motility dysfunction is present not only during bowel obstruction (BO), but after obstruction is resolved. Previous studies found that lumen distension associated mechano-transcription of COX-2 and production of PGE2 in gut smooth muscle cells (SMC) account for motility dysfunction during obstruction. We hypothesized ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27230-6
更新日期：2018-06-11 00:00:00
abstract：:Combined inhibition of BRAF and MEK1/2 (CIBM) improves therapeutic efficacy of BRAF-mutant melanoma. However, drug resistance to CIBM is inevitable and the drug resistance mechanisms still remain to be elucidated. Here, we show that BMK1 pathway contributes to the drug resistance to CIBM. Considering that ERK1/2 pathw...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46244
更新日期：2017-04-07 00:00:00
abstract：:Here, we report large, near-daily oscillations of near-bottom temperatures, with ranges of up to 11.5 °C at depths of 30-60 m in September 2011 around the Saint Pierre and Miquelon archipelago (south-eastern Canada). These oscillations were detected on velocity and temperature profiles from moorings in Miquelon Bay an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31857-w
更新日期：2018-09-17 00:00:00
abstract：:The ability to harvest thermal energy and manipulate heat fluxes has recently attracted a great deal of research interest because this is critical to achieve efficient solar-to-thermal energy conversion in the technology of concentrated solar thermal collectors. Thermal metamaterials with engineered thermal conduction...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11552
更新日期：2015-06-25 00:00:00
abstract：:The monitoring and early detection of abnormalities or variations in the cardiac cycle functionality are very critical practices and have significant impact on the prevention of heart diseases and their associated complications. Currently, in the field of biomedical engineering, there is a growing need for devices cap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37093
更新日期：2016-11-17 00:00:00
abstract：:Sweet sorghum bagasse (SSB) is a promising raw material for silage fermentation due to its high residual nutritive, but the efficient fermentation strategy of SSB has not been reported yet. This study evaluated the effects of microbial inoculant on the fermentation quality, chemical composition and microbial community...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57628-0
更新日期：2020-01-21 00:00:00
abstract：:Modeling the dynamic nature of protein-ligand binding with atomistic simulations is one of the main challenges in computational biophysics, with important implications in the drug design process. Although in the past few years hardware and software advances have significantly revamped the use of molecular simulations,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08445-5
更新日期：2017-08-16 00:00:00
abstract：:Osteoporosis is associated with reduced density and quality of bone leading to weakened skeleton thereby increasing the risk of fractures responsible for increased morbidity and mortality. Due to preference for western food style the consumption of salt intake in our diets has increased many folds. High dietary salt i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20896-y
更新日期：2018-02-06 00:00:00
abstract：:Stability and integration are the goals for developing photonic devices. Spirooxazines have the property of photoinduced merocyanine-aggregation in polymer matrix, which can be applied to fluorescence emission and stable information storage. Although visible light coherent radiation with UV-assist has been used to ach...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22202-2
更新日期：2018-02-28 00:00:00
abstract：:We investigated the association of redox regulator Nuclear factor erythroid 2-related factor 2 (Nrf2) and inflammatory cytokines as well as clinical remission in patients with recent onset type 2 diabetes (DM). Blood was collected from 180 DM patients (105 males/75 females) and 150 control subjects (86 males/64 female...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-22913-6
更新日期：2018-03-23 00:00:00
abstract：:Multiple parasitic arthropods of medical importance depend on symbiotic bacteria. While the link between scabies and secondary bacterial infections causing post infective complications of Group A streptococcal and staphylococcal pyoderma is increasingly recognized, very little is known about the microbiota of Sarcopte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47892-0
更新日期：2019-08-13 00:00:00
abstract：:Understanding the transmission patterns of African swine fever (ASF) among wild boar (Sus scrofa) is an issue of major interest, especially in the wake of the current ASF epidemic. Given the high stability of ASF-virus, there is concern about scavengers spreading infectious carcass material in the environment. Here, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47623-5
更新日期：2019-08-07 00:00:00
abstract：:Triboelectric charging is common in desert sandstorms and dust devils on Earth; however, it remains poorly understood. Here we show a charging mechanism of sands with the adsorbed water on micro-porous surface in wind-blown sand based on the fact that water content is universal but usually a minor component in most pa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01337
更新日期：2013-01-01 00:00:00
