Abstract：
:Pseudooxynicotine amine oxidase (Pnao) is essential to the pyrrolidine pathway of nicotine degradation of Pseudomonas putida strain S16, which is significant for the detoxification of nicotine, through removing the CH3NH2 group. However, little is known about biochemical mechanism of this enzyme. Here, we characterized its properties and biochemical mechanism. Isotope labeling experiments provided direct evidence that the newly introduced oxygen atom in 3-succinoylsemialdehyde-pyridine is derived from H2O, but not from O2. Pnao was very stable at temperatures below 50 °C; below this temperature, the enzyme activity increased as temperature rose. Site-directed mutagenesis studies showed that residue 180 is important for its thermal stability. In addition, tungstate may enhance the enzyme activity, which has rarely been reported before. Our findings make a further understanding of the crucial Pnao in nicotine degradation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hu H,Wang W,Tang H,Xu Pdoi
10.1038/srep17770subject
Has Abstractpub_date
2015-12-04 00:00:00pages
17770issn
2045-2322pii
srep17770journal_volume
5pub_type
杂志文章abstract：:Approximately 300,000 men around the globe self-identify as Ashkenazi Levites, of whom two thirds were previously shown to descend from a single male. The paucity of whole Y-chromosome sequences precluded conclusive identification of this ancestor's age, geographic origin and migration patterns. Here, we report the va...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14761-7
更新日期：2017-11-02 00:00:00
abstract：:The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08542
更新日期：2015-02-25 00:00:00
abstract：:Colloidal based films have been widely developed for a wide range of applications including chemical and electrical barrier coatings, photonic materials, biomaterials, and pharmaceutical oral drug delivery. Many previous studies investigate methods to generate uniformity or desired stratification of the final componen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66875-0
更新日期：2020-06-24 00:00:00
abstract：:In spite of its prevalence in animals and plants, endogenous nitric oxide (NO) has been rarely reported in fungi. We present here our observations on production of intracellular NO and its possible roles during development of Neurospora crassa, a model filamentous fungus. Intracellular NO was detected in hypha 8-16 ho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30037
更新日期：2016-07-18 00:00:00
abstract：:Remote earthquake triggering is a well-established phenomenon. Triggering is commonly identified from statistically significant increases in earthquake rate coincident with the passage of seismic energy. In establishing rate changes, short duration earthquake catalogs are commonly used, and triggered sequences are not...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60988-2
更新日期：2020-03-05 00:00:00
abstract：:Human mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME) has a dimer of dimers quaternary structure with two independent allosteric sites in each monomer. Here, we reveal the different effects of nucleotide ligands on the quaternary structure regulation and functional role of the human m-NAD(P)-ME exosite. In ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45282-0
更新日期：2019-06-24 00:00:00
abstract：:The over-expression and aggregation of α-synuclein (αSyn) are linked to the onset and pathology of Parkinson's disease. Native monomeric αSyn exists in an intrinsically disordered ensemble of interconverting conformations, which has made its therapeutic targeting by small molecules highly challenging. Nonetheless, her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52598-4
更新日期：2019-11-18 00:00:00
abstract：:A hypereutectic Al-Fe-Cu alloy with a high-volume fraction ferro-aluminum second phase (AlFe phases for short) was reheated in the solid-liquid region and the microstructure evolution was investigated. During semisolid heating, the high-melting AlFe phases in the Al-Fe-Cu alloy were demonstrated to stunt the grain gro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-80983-5
更新日期：2021-01-15 00:00:00
abstract：:Glyoxalase pathway plays an important role in stress adaptation and many clinical disorders. The first enzyme of this pathway, glyoxalase I (GlxI), uses methylglyoxal as a substrate and requires either Ni(II)/Co(II) or Zn(II) for activity. Here we have investigated the origin of different metal ion specificities of Gl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03076
更新日期：2013-11-13 00:00:00
abstract：:Optical projection tomography (OPT) is a tool used for three-dimensional imaging of millimeter-scale biological samples, with the advantage of exhibiting isotropic resolution typically in the micron range. OPT can be divided into two types: transmission OPT (tOPT) and emission OPT (eOPT). Compared with eOPT, tOPT disc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19241
更新日期：2016-01-11 00:00:00
abstract：:Adropin is a peptide hormone encoded by the Energy Homeostasis Associated (ENHO) gene whose physiological role in humans remains incompletely defined. Here we investigated the impact of dietary interventions that affect systemic glucose and lipid metabolism on plasma adropin concentrations in humans. Consumption of gl...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/srep14691
更新日期：2015-10-05 00:00:00
abstract：:Diversification between islands and ecological radiation within islands are postulated to have occurred in the Euphorbia species (sect. Aphyllis subsect. Macaronesicae) on the Canary Islands. In this study, the biogeographical pattern of 11 species of subsect. Macaronesicae and the genetic differentiation among five s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34454
更新日期：2016-09-29 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-69834-x
更新日期：2020-07-29 00:00:00
abstract：:A novel one-pot synthesis of tri-substituted purines and the discovery of purine analogues with trypanocidal activity are reported. The reaction is initiated by a metal-free oxidative coupling of primary alkoxides and diaminopyrimidines with Schiff base formation and subsequent annulation in the presence of large N,N-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09139
更新日期：2015-03-16 00:00:00
abstract：:Congenital reproductive tract anomalies could impair fertility. Female and male reproductive tracts are developed from Müllerian ducts and Wolffian ducts, respectively, involving initiation, elongation and differentiation. Genetic basis solely for distal reproductive tract development is largely unknown. Lhfpl2 (lipom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23037
更新日期：2016-03-11 00:00:00
abstract：:Transmission electron microscopy and related analytical techniques have been widely used to study the microstructure of different materials. However, few research works have been performed in the field of glasses, possibly due to the electron-beam irradiation damage. In this paper, we have developed a method based on ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17526
更新日期：2015-12-08 00:00:00
abstract：:A variety of environmental factors contribute to progressive development of osteoarthritis (OA). Environmental factors that upset circadian rhythms have been linked to various diseases. Our recent work establishes chronic environmental circadian disruption - analogous to rotating shiftwork-associated disruption of cir...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16896
更新日期：2015-11-20 00:00:00
abstract：:This paper reports on 3D phase field simulations of IMC growth in Co/Sn and Cu/Sn solder systems. In agreement with experimental micrographs, we obtain uniform growth of the CoSn3 phase in Co/Sn solder joints and a non-uniform wavy morphology for the Cu6Sn5 phase in Cu/Sn solder joints. Furthermore, simulations were p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51179-9
更新日期：2019-10-16 00:00:00
abstract：:Speckle, amplitude fluctuations in optical coherence tomography (OCT) images, contains information on sub-resolution structural properties of the imaged sample. Speckle statistics could therefore be utilized in the characterization of biological tissues. However, a rigorous theoretical framework relating OCT speckle s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14115-3
更新日期：2017-11-01 00:00:00
abstract：:Non-forest ecosystems (predominant in semi-arid and arid regions) contribute significantly to the increasing trend and interannual variation of land carbon uptake over the last three decades, yet the mechanisms are poorly understood. By analysing the flux measurements from 23 ecosystems in Australia, we found the the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11063-w
更新日期：2017-09-15 00:00:00
abstract：:Pulse generation often requires a stabilized cavity and its corresponding mode structure for initial phase-locking. Contrastingly, modeless cavity-free random lasers provide new possibilities for high quantum efficiency lasing that could potentially be widely tunable spectrally and temporally. Pulse generation in rand...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18526
更新日期：2015-12-21 00:00:00
abstract：:The 170 National Forests and Grasslands (NFs) in the conterminous United States are public lands that provide important ecosystem services such as clean water and timber supply to the American people. This study investigates the potential impacts of climate change on two key ecosystem functions (i.e., water yield and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24441
更新日期：2016-04-21 00:00:00
abstract：:Nanometric biocompatible Metal-Organic Frameworks (nanoMOFs) are promising candidates for drug delivery. Up to now, most studies have targeted the intravenous route, related to pain and severe complications; whereas nanoMOFs for oral administration, a commonly used non-invasive and simpler route, remains however unexp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43099
更新日期：2017-03-03 00:00:00
abstract：:Animals achieve camouflage through a variety of mechanisms, of which background matching and disruptive coloration are likely the most common. Although many studies have investigated camouflage mechanisms using artificial stimuli and in lab experiments, less work has addressed camouflage in the wild. Here we examine e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32059
更新日期：2016-09-12 00:00:00
abstract：:Prescribing the right drug with the right dose is a central tenet of precision medicine. We examined the use of patients' prior Electronic Health Records to predict a reduction in drug dosage. We focus on drugs that interact with the P450 enzyme family, because their dosage is known to be sensitive and variable. We ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33980-0
更新日期：2018-10-22 00:00:00
abstract：:Users of social media often share their feelings or emotional states through their posts. In this study, we developed a deep learning model to identify a user's mental state based on his/her posting information. To this end, we collected posts from mental health communities in Reddit. By analyzing and learning posting...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68764-y
更新日期：2020-07-16 00:00:00
abstract：:Post-glycosylphosphatidylinositol (GPI) attachment to proteins 3, also known as PGAP3 or PERLD1 (PER1-like domain-containing protein 1), participates in the lipid remodeling process of glycosylphosphatidylinositol (GPI) anchor proteins during post-translational modification. Functional defect in PERLD1 was previously ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57592-9
更新日期：2020-01-20 00:00:00
abstract：:The characterization of network community structure has profound implications in several scientific areas. Therefore, testing the algorithms developed to establish the optimal division of a network into communities is a fundamental problem in the field. We performed here a highly detailed evaluation of community detec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02216
更新日期：2013-01-01 00:00:00
abstract：:The COVID-19 pandemic caused by the SARS-CoV-2 requires a fast development of antiviral drugs. SARS-CoV-2 viral main protease (Mpro, also called 3C-like protease, 3CLpro) is a potential target for drug design. Crystal and co-crystal structures of the SARS-CoV-2 Mpro have been solved, enabling the rational design of in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77794-5
更新日期：2020-11-30 00:00:00
abstract：:Silicene is an exciting two-dimensional material that shares many of graphene's electronic properties, but differs in its structural buckling. This buckling allows opening a bandgap in silicene through the application of a perpendicular electric field. Here we show that this buckling also enables highly effective modu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14815
更新日期：2015-10-06 00:00:00
