Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.

Abstract:

:The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a total of 185 fresh gastroscopic biopsies of gastric cancer were subcutaneously transplanted into NOD/SCID (Nonobese Diabetic/Severe Combined Immunodeficiency) mice. Sixty-three PDTX models were successfully established (34.1%, 63/185) and passaged to maintain tumors in vivo, and the mean latency period of xenografts was 65.86 ± 32.84 days (11-160 days). Biopsies of prior chemotherapy had a higher transplantation rate (52.1%, 37/71) than biopsies after chemotherapy (21.9%, 25/114; P = 0.000). No differences were found between the latency period of xenografts and characteristics of patients. The pathological and molecular features of PDTX as well as chemosensitivity were highly consistent with those of primary tumors of patients. The genetic characteristics were stable during passaging of PDTX models. In summary PDTX models using gastroscopic biopsies in gastric cancer were demonstrated for the first time, and the biological characteristics of the PDTX models were highly consistent with patients, which provided the best preclinical study platform for gastric cancer.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Zhu Y,Tian T,Li Z,Tang Z,Wang L,Wu J,Li Y,Dong B,Li Y,Li N,Zou J,Gao J,Shen L

doi

10.1038/srep08542

subject

Has Abstract

pub_date

2015-02-25 00:00:00

pages

8542

issn

2045-2322

pii

srep08542

journal_volume

5

pub_type

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