Identification and functional analysis of endogenous nitric oxide in a filamentous fungus.

Abstract:

:In spite of its prevalence in animals and plants, endogenous nitric oxide (NO) has been rarely reported in fungi. We present here our observations on production of intracellular NO and its possible roles during development of Neurospora crassa, a model filamentous fungus. Intracellular NO was detected in hypha 8-16 hours after incubation in Vogel's minimal liquid media and conidiophores during conidiation using a fluorescent indicator (DAF-FM diacetate). Treatment with cPTIO, an NO scavenger, significantly reduced fluorescence levels and hindered hyphal growth in liquid media and conidiation, whereas exogenous NO enhanced hyphal extension on VM agar media and conidia formation. NO scavenging also dramatically diminished transcription of con-10 and con-13, genes preferentially expressed during conidiation. Our results suggest that intracellular NO is generated in young hypha growing in submerged culture and during conidia development and regulate mycelial development and conidia formation.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Pengkit A,Jeon SS,Son SJ,Shin JH,Baik KY,Choi EH,Park G

doi

10.1038/srep30037

subject

Has Abstract

pub_date

2016-07-18 00:00:00

pages

30037

issn

2045-2322

pii

srep30037

journal_volume

6

pub_type

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