Abstract：
:The Gram-negative bacterium Pseudomonas aeruginosa represents a prototype of multi-drug resistant opportunistic pathogens for which novel therapeutic options are urgently required. In order to identify new candidates as potential drug targets, we combined large-scale transposon mutagenesis data analysis and bioinformatics predictions to retrieve a set of putative essential genes which are conserved in P. aeruginosa and predicted to encode cell envelope or secreted proteins. By generating unmarked deletion or conditional mutants, we confirmed the in vitro essentiality of two periplasmic proteins, LptH and LolA, responsible for lipopolysaccharide and lipoproteins transport to the outer membrane respectively, and confirmed that they are important for cell envelope stability. LptH was also found to be essential for P. aeruginosa ability to cause infection in different animal models. Conversely, LolA-depleted cells appeared only partially impaired in pathogenicity, indicating that this protein likely plays a less relevant role during bacterial infection. Finally, we ruled out any involvement of the other six proteins under investigation in P. aeruginosa growth, cell envelope stability and virulence. Besides proposing LptH as a very promising drug target in P. aeruginosa, this study confirms the importance of in vitro and in vivo validation of potential essential genes identified through random transposon mutagenesis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Fernández-Piñar R,Lo Sciuto A,Rossi A,Ranucci S,Bragonzi A,Imperi Fdoi
10.1038/srep17593subject
Has Abstractpub_date
2015-12-01 00:00:00pages
17593issn
2045-2322pii
srep17593journal_volume
5pub_type
杂志文章abstract：:Membranes with selective superwettability for oil/water separation have received significant attention during the past decades. Hierarchical structures and surface roughness are believed to improve the oil repellency and the stability of Cassie-Baxter state. Diatoms, unicellular photosynthetic algae, possess sophistic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01642-2
更新日期：2017-05-03 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2020-01-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58783-0
更新日期：2020-02-04 00:00:00
abstract：:Valproic acid (VPA) is a drug commonly used for epileptic seizure control. Recently, it has been shown that VPA alters the activation of several immune cells, including Natural Killer (NK) cells, which play an important role in the containment of viruses and intracellular bacteria. Although VPA can increase susceptibi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74836-w
更新日期：2020-10-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36346
更新日期：2016-11-03 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-27 00:00:00
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pub_type： 已发布勘误
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更新日期：2020-02-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-09-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-09-10 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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更新日期：2015-06-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-01-16 00:00:00
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pub_type： 杂志文章
doi：10.1038/s41598-020-78880-4
更新日期：2020-12-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49617-9
更新日期：2019-09-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19222-3
更新日期：2018-01-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-019-51522-0
更新日期：2019-10-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38641
更新日期：2016-12-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24883-1
更新日期：2018-04-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38171-5
更新日期：2019-02-04 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71074-y
更新日期：2020-09-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76593-2
更新日期：2020-11-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22892-8
更新日期：2018-03-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28989-4
更新日期：2018-07-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50237-6
更新日期：2019-09-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35650-7
更新日期：2018-11-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30751
更新日期：2016-08-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05089-3
更新日期：2017-07-10 00:00:00
abstract：:We present new evidence that shows magma mingling can be a key process during highly explosive eruptions. Using fractal analysis of the size distribution of trachybasaltic fragments found on the inner walls of bubbles in trachytic pumices, we show that the more mafic component underwent fracturing during quenching aga...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17098-3
更新日期：2017-12-04 00:00:00
abstract：:Clinical observations suggest neuronal control of bone remodeling. Sensory nerve fibers innervating bone, bone marrow and periosteum signal via neurotransmitters including substance P (SP). In previous studies we observed impaired biomechanical and structural bone parameters in tachykinin (Tac) 1-deficient mice lackin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27432-y
更新日期：2018-06-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42644
更新日期：2017-02-16 00:00:00
abstract：:Photoreceptor degeneration due to retinitis pigmentosa (RP) is a primary cause of inherited retinal blindness. Photoreceptor cell-replacement may hold the potential for repair in a completely degenerate retina by reinstating light sensitive cells to form connections that relay information to downstream retinal layers....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29784
更新日期：2016-07-13 00:00:00
