Abstract:
:The demand for phenomics, a high-dimensional and high-throughput phenotyping method, has been increasing in many fields of biology. The budding yeast Saccharomyces cerevisiae, a unicellular model organism, provides an invaluable system for dissecting complex cellular processes using high-resolution phenotyping. Moreover, the addition of spatial and temporal attributes to subcellular structures based on microscopic images has rendered this cell phenotyping system more reliable and amenable to analysis. A well-designed experiment followed by appropriate multivariate analysis can yield a wealth of biological knowledge. Here we review recent advances in cell imaging and illustrate their broad applicability to eukaryotic cells by showing how these techniques have advanced our understanding of budding yeast.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Ohya Y,Kimori Y,Okada H,Ohnuki Sdoi
10.1091/mbc.E15-07-0466subject
Has Abstractpub_date
2015-11-05 00:00:00pages
3920-5issue
22eissn
1059-1524issn
1939-4586pii
26/22/3920journal_volume
26pub_type
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